{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T02:22:12Z","timestamp":1789006932185,"version":"build-2803163510"},"reference-count":58,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,2,12]],"date-time":"2021-02-12T00:00:00Z","timestamp":1613088000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:p>Dengue is an acute viral disease caused by dengue virus (DENV), which is transmitted by <jats:italic>Aedes<\/jats:italic> mosquitoes. Symptoms of DENV infection range from inapparent to severe and can be life-threatening. DENV replicates in primary immune cells such as dendritic cells and macrophages, which contribute to the dissemination of the virus. Susceptibility of other immune cells such as B cells to direct infection by DENV and their subsequent response to infection is not well defined. In a cohort of 60 Cambodian children, we showed that B cells are susceptible to DENV infection. Moreover, we show that B cells can support viral replication of laboratory adapted and patient-derived DENV strains. B cells were permissive to DENV infection albeit low titers of infectious virions were released in cell supernatants CD300a, a phosphatidylserine receptor, was identified as a potential attachment factor or receptor for entry of DENV into B cells. In spite of expressing Fc<jats:italic>\u03b3<\/jats:italic>-receptors, antibody-mediated enhancement of DENV infection was not observed in B cells in an <jats:italic>in vitro<\/jats:italic> model. Direct infection by DENV induced proliferation of B cells in dengue patients <jats:italic>in vivo<\/jats:italic> and plasmablast\/plasma cell formation <jats:italic>in vitro<\/jats:italic>. To summarize, our results show that B cells are susceptible to direct infection by DENV <jats:italic>via<\/jats:italic> CD300a and the subsequent B cell responses could contribute to dengue pathogenesis.<\/jats:p>","DOI":"10.3389\/fimmu.2020.594813","type":"journal-article","created":{"date-parts":[[2021,2,13]],"date-time":"2021-02-13T19:51:30Z","timestamp":1613245890000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":31,"title":["Direct Infection of B Cells by Dengue Virus Modulates B Cell Responses in a Cambodian Pediatric Cohort"],"prefix":"10.3389","volume":"11","author":[{"given":"Vinit","family":"Upasani","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hoa Thi 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