{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T02:43:03Z","timestamp":1790390583619,"version":"4.1.0"},"reference-count":49,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,2,12]],"date-time":"2021-02-12T00:00:00Z","timestamp":1613088000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2016\/05617-4, 2017\/11865-3 , 2018\/20403-6"],"award-info":[{"award-number":["2016\/05617-4, 2017\/11865-3 , 2018\/20403-6"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:p>Patients with Systemic sclerosis (SSc) presents immune dysregulation, vasculopathy, and fibrosis of the skin and various internal organs. Pulmonary fibrosis leads to SSc-associated interstitial lung disease (ILD), which is the main cause of morbidity and mortality in SSc. Recently autoimmunity to type V collagen (Col V) has been characterized in idiopathic pulmonary fibrosis and show promise to be related to the development in SSc. Our aim was to evaluate autoimmunity to Col V \u03b11(V) and \u03b12(V) chains and to the antigenic peptides of these Col V chains in early-SSc sera employing lung tissue of SSc-ILD, as antigen source. We found that sera samples from patients with early-SSc were reactive to Col V (41.18%) and presented immunoreactivity for Col5A1(1.049) and Col5A1(1.439) peptides. The IgG isolated from early-SSc patients-anti-Col V positive sera (anti-ColV IgG) was adsorbed with \u03b11(V) chain (anti-ColV IgG\/ads-\u03b11(V)) and \u03b12(V) chain (anti-ColV IgG\/ads-\u03b12(V)) and biotinylated to evaluate the spectrum of reactivity in SSc-ILD patients lung biopsies by immunofluorescence. The SSc-ILD lung tissue samples immunostained with anti-ColV IgG showed increased green fluorescence in the vascular basement membrane, bronchiolar smooth muscle, and adventitial layer, contrasting with the tenue immunostaining in control lungs. Col V protein expression in these pulmonary compartments immunostained with early-SSc anti-ColV IgG was confirmed by immune colocalization assays with commercial anti-human Col V antibodies. In addition, SSc-ILD lung tissues immunostained with anti-ColV IgG\/ads-\u03b11(V) (sample in which Col V \u03b11 chain-specific antibodies were removed) showed decreased green fluorescence compared to anti-ColV IgG and anti-ColV IgG\/ads-\u03b12(V). Our data show that autoimmunity to Col V in early-SSc was related to peptides of the \u03b11(V) chain, suggesting that these antibodies could be biomarkers of SSc stages and potential target of immunotherapy with Col V immunogenic peptides.<\/jats:p>","DOI":"10.3389\/fimmu.2020.604602","type":"journal-article","created":{"date-parts":[[2021,2,13]],"date-time":"2021-02-13T22:16:45Z","timestamp":1613254605000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":14,"title":["Identification of Autoimmunity to Peptides of Collagen V \u03b11 Chain as Newly Biomarkers of Early Stage of Systemic Sclerosis"],"prefix":"10.3389","volume":"11","author":[{"given":"Ana Paula Pereira","family":"Velosa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lais","family":"Brito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zelita Aparecida","family":"de Jesus Queiroz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Solange","family":"Carrasco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jurandir","family":"Tomaz de Miranda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cec\u00edlia","family":"Farhat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cl\u00e1udia","family":"Goldenstein-Schainberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edwin Roger","family":"Parra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danieli Castro Oliveira","family":"de Andrade","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro Leme","family":"Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vera Luiza","family":"Capelozzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walcy Rosolia","family":"Teodoro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2021,2,12]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2009.114264","article-title":"Causes and risk factors for death in systemic sclerosis: a study from the EULAR Scleroderma Trials and Research (EUSTAR) database","volume":"69","author":"Tyndall","year":"2010","journal-title":"Ann Rheum Dis"},{"key":"B2","doi-asserted-by":"publisher","first-page":"42","DOI":"10.1007\/s11926-020-00918-3","article-title":"Current and Potential New Targets in Systemic Sclerosis Therapy: a New Hope","volume":"22","author":"Hinchcliff","year":"2020","journal-title":"Curr Rheumatol Rep"},{"key":"B3","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1016\/j.addr.2015.11.001","article-title":"Extracellular matrix structure","volume":"97","author":"Theocharis","year":"2016","journal-title":"Adv Drug Delivery Rev"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.023820","article-title":"The extracellular matrix at a glance","volume":"123","author":"Frantz","year":"2010","journal-title":"J Cell Sci"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1111\/1346-8138.14153","article-title":"Systemic sclerosis","volume":"45","author":"Asano","year":"2018","journal-title":"J Dermatol"},{"key":"B6","doi-asserted-by":"crossref","DOI":"10.1242\/jcs.95.4.649","article-title":"Collagen fibrillogenesis in vitro: interaction of types I and V collagen regulates fibril diameter","volume":"95","author":"Birk","year":"1990","journal-title":"J Cell Sci"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.121.5.1181","article-title":"Type V collagen: Molecular structure and fibrillar organization of the chicken \u03b11(V) NH2-terminal domain, a putative regulator of corneal fibrillogenesis","volume":"121","author":"Linsenmayer","year":"1993","journal-title":"J\u00a0Cell Biol"},{"key":"B8","doi-asserted-by":"publisher","first-page":"62","DOI":"10.1165\/ajrcmb.23.1.3924","article-title":"Type V collagen modulates alloantigen-induced pathology and immunology in the lung","volume":"23","author":"Mares","year":"2000","journal-title":"Am J Respir Cell Mol Biol"},{"key":"B9","first-page":"1","article-title":"Structure and function of collagen types","volume-title":"Biology of Extracellular Matrix. 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