{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T01:38:48Z","timestamp":1792028328977,"version":"4.4.0"},"reference-count":77,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2020,12,16]],"date-time":"2020-12-16T00:00:00Z","timestamp":1608076800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["AEI\/EU-FEDER RTI2018-095997-B-I00"],"award-info":[{"award-number":["AEI\/EU-FEDER RTI2018-095997-B-I00"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010769","name":"Axencia Galega de Innovaci\u00f3n","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010769","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100013276","name":"Interreg","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100013276","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007601","name":"Horizon 2020","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007601","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:p>Mussels (<jats:italic>Mytilus galloprovincialis<\/jats:italic>) are filter feeder bivalves that are constantly in contact with a wide range of microorganisms, some of which are potentially pathogenic. How mussels recognize and respond to pathogens has not been fully elucidated to date; therefore, we investigated the immune mechanisms that these animals employ in response to a bacterial bath infection from the surrounding water, mimicking the response that mussels mount under natural conditions. After the bath infection, mussels were able to remove the bacteria from their bodies and from the water tank. Accordingly, antibacterial activity was detected in gill extracts, demonstrating that this tissue plays a central role in removing and clearing potential pathogens. A transcriptomic study performed after a bath infection with <jats:italic>Vibrio splendidus<\/jats:italic> identified a total of 1,156 differentially expressed genes. The expression levels of genes contributing to a number of biological processes, such as immune response activation pathways and their regulation with cytokines, cell recognition, adhesion and apoptosis, were significantly modulated after infection, suggesting that the gills play important roles in pathogen recognition, as well as being activators and regulators of the mussel innate immune response. In addition to RNA-seq analysis, long non-coding RNAs and their neighboring genes were also analyzed and exhibited modulation after the bacterial challenge. The response of gills against bath infection was compared with the findings of a previous transcriptomic study on hemocytes responding to systemic infection, demonstrating the different and specific functions of gills. The results of this study indicate that recognition processes occur in the gill, thereby activating the effector agents of the immune response to overcome bacterial infection.<\/jats:p>","DOI":"10.3389\/fimmu.2020.615580","type":"journal-article","created":{"date-parts":[[2021,1,10]],"date-time":"2021-01-10T12:21:02Z","timestamp":1610281262000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":94,"title":["Transcriptomic Response of Mussel Gills After a Vibrio splendidus Infection Demonstrates Their Role in the Immune Response"],"prefix":"10.3389","volume":"11","author":[{"given":"Amaro","family":"Saco","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magal\u00ed","family":"Rey-Campos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz","family":"Novoa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Figueras","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2020,12,16]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.3354\/meps07033","article-title":"Structural and functional effects of Mytilus edulis on diversity of associated species and ecosystem functioning","volume":"351","author":"Norling","year":"2007","journal-title":"Mar Ecol Prog Ser"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gloplacha.2016.08.002","article-title":"Retrospective environmental biomonitoring \u2013 Mussel Watch expanded","volume":"144","author":"Sch\u00f6ne","year":"2016","journal-title":"Glob Planet Change"},{"key":"B3","unstructured":"La Acuicultura en Espa\u00f1a 20192019"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2010.07.011","article-title":"Detection and description of a particular Ostreid herpesvirus 1 genotype associated with massive mortality outbreaks of Pacific oysters, Crassostrea gigas, in France in 2008","volume":"153","author":"Segarra","year":"2010","journal-title":"Virus Res"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.3354\/dao02701","article-title":"Occurrence, seasonality and infectivity of Vibrio strains in natural populations of mussels Mytilus galloprovincialis","volume":"108","author":"Romero","year":"2014","journal-title":"Dis Aquat Organ"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2018.06.075","article-title":"Mortality investigation of Mytilus edulis and Mytilus galloprovincialis in France: An experimental survey under laboratory conditions","volume":"495","author":"Benabdelmouna","year":"2018","journal-title":"Aquaculture"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1016\/j.jip.2018.02.018","article-title":"Assessing the health status of farmed mussels (Mytilus galloprovincialis) through histological, microbiological and biomarker analyses","volume":"153","author":"Matozzo","year":"2018","journal-title":"J Invertebr Pathol"},{"key":"B8","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1007\/s00227-018-3308-0","article-title":"Bivalve transcriptomics reveal pathogen sequences and a powerful immune response of the Mediterranean mussel (Mytilus galloprovincialis)","volume":"165","author":"Moreira","year":"2018","journal-title":"Mar Biol"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2012.01146.x","article-title":"PAMPs and DAMPs: signal 0s that spur autophagy and immunity","volume":"249","author":"Tang","year":"2012","journal-title":"Immunol Rev"},{"key":"B10","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1189\/jlb.0306164","article-title":"DAMPs, PAMPs and alarmins: all we need to know about danger","volume":"81","author":"Bianchi","year":"2007","journal-title":"J Leukoc Biol"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2009.02.001","article-title":"Functional and molecular immune response of Mediterranean mussel (Mytilus galloprovincialis) haemocytes against pathogen-associated molecular patterns and bacteria","volume":"26","author":"Costa","year":"2009","journal-title":"Fish Shellfish Immunol"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.01894","article-title":"Immune tolerance in Mytilus galloprovincialis hemocytes after repeated contact with Vibrio splendidus","volume":"10","author":"Rey-Campos","year":"2019","journal-title":"Front Immunol"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2003.09.009","article-title":"Flow cytometry as a tool to quantify oyster defence mechanisms","volume":"16","author":"Goedken","year":"2004","journal-title":"Fish Shellfish Immunol"},{"key":"B14","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1016\/S0145-305X(02)00098-8","article-title":"Nitric oxide production by carpet shell clam (Ruditapes decussatus) hemocytes","volume":"27","author":"Tafalla","year":"2003","journal-title":"Dev Comp Immunol"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1996.0302h.x","article-title":"A member of the arthropod defensin family from edible Mediterranean mussels (Mytilus galloprovincialis)","volume":"240","author":"Hubert","year":"1996","journal-title":"Eur J Biochem"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.1999.00654.x","article-title":"Myticin, a novel cysteine-rich antimicrobial peptide\u00a0isolated from haemocytes and plasma of the mussel Mytilus galloprovincialis","volume":"265","author":"Mitta","year":"1999","journal-title":"Eur J Biochem"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.17.12954","article-title":"Involvement of mytilins in mussel antimicrobial defense","volume":"275","author":"Mitta","year":"2000","journal-title":"J Biol Chem"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1007\/s10126-010-9345-4","article-title":"Diversity of coding sequences and gene structures of the antifungal peptide mytimycin (MytM) from the Mediterranean mussel, Mytilus galloprovincialis","volume":"13","author":"Sonthi","year":"2011","journal-title":"Mar Biotechnol"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2011.08.003","article-title":"Big defensins and mytimacins, new AMP families of the Mediterranean mussel Mytilus galloprovincialis","volume":"36","author":"Gerdol","year":"2012","journal-title":"Dev Comp Immunol"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2012.11.030","article-title":"Molecular characterization of a novel antimicrobial peptide from\u00a0Mytilus coruscus","volume":"34","author":"Liao","year":"2013","journal-title":"Fish Shellfish Immunol"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2014.09.019","article-title":"Characterization of a novel antimicrobial peptide with chiting-biding domain from Mytilus coruscus","volume":"41","author":"Qin","year":"2014","journal-title":"Fish Shellfish Immunol"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.3390\/md15080261","article-title":"Myticalins: A novel multigenic family of linear, cationic antimicrobial peptides from marine mussels (Mytilus spp.)","volume":"15","author":"Leoni","year":"2017","journal-title":"Mar Drugs"},{"key":"B23","doi-asserted-by":"crossref","DOI":"10.1016\/B978-0-12-417186-2.00012-1","article-title":"Mucosal immunity in mollusks","volume-title":"Mucosal Health in Aquaculture","author":"Allam","year":"2015"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-005-1093-9","article-title":"Characterization of mussel gill cells in vivo and in vitro","volume":"321","author":"G\u00f3mez-Mendikute","year":"2005","journal-title":"Cell Tissue Res"},{"key":"B25","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1007\/978-3-319-25172-1_6","article-title":"Dissection of a Freshwater Mussel (Anodonta anatina)","volume-title":"Atlas of Animal Anatomy and Histology","author":"L\u0151w","year":"2016"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2013.09.027","article-title":"Interactions between Mytilus galloprovincialis hemocytes and the bivalve pathogens Vibrio aestuarianus 01\/032 and Vibrio splendidus LGP32","volume":"35","author":"Balbi","year":"2013","journal-title":"Fish Shellfish Immunol"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2017.07.002","article-title":"Development of a duplex Taqman real-time PCR assay for rapid identification of Vibrio splendidus-related and V. aestuarianus strains from bacterial cultures","volume":"140","author":"Saulnier","year":"2017","journal-title":"J Microbiol Methods"},{"key":"B28","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1186\/s13059-020-02180-3","article-title":"Massive gene presence-absence variation shapes an open pan-genome in the Mediterranean mussel","volume":"21","author":"Gerdol","year":"2020","journal-title":"Genome Biol"},{"key":"B29","doi-asserted-by":"publisher","first-page":"e74","DOI":"10.1093\/nar\/gkt006","article-title":"CPAT: Coding-Potential Assessment Tool using an alignment-free logistic regression model","volume":"41","author":"Wang","year":"2013","journal-title":"Nucleic Acids Res"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv468","article-title":"ClustVis: a web tool for visualizing clustering of multivariate data using Principal Component Analysis and heatmap","volume":"43","author":"Metsalu","year":"2015","journal-title":"Nucleic Acids Res"},{"key":"B31","unstructured":"OliverosJC\n          VENNY. An interactive tool for comparing lists with Venn Diagrams2007"},{"key":"B32","doi-asserted-by":"publisher","first-page":"3569","DOI":"10.1038\/s41598-019-39870-3","article-title":"High individual variability in the transcriptomic response of Mediterranean mussels to Vibrio reveals the involvement of myticins in tissue injury","volume":"9","author":"Rey-Campos","year":"2019","journal-title":"Sci Rep"},{"key":"B33","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1093\/nar\/gkn923","article-title":"Bioinformatics enrichment tools: paths toward the comprehensive functional analysis of large gene lists","volume":"37","author":"Huang","year":"2008","journal-title":"Nucleic Acids Res"},{"key":"B34","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1038\/nprot.2008.211","article-title":"Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources","volume":"4","author":"Huang","year":"2009","journal-title":"Nat Protoc"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.9.e45","article-title":"A new mathematical model for relative quantification in real-time RT\u2013PCR","volume":"29","author":"Pfaffl","year":"2001","journal-title":"Nucleic Acids Res"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1080\/10641260802325518","article-title":"Study of diseases and the immune system of bivalves using molecular biology and genomics","volume":"16","author":"Gestal","year":"2008","journal-title":"Rev Fish Sci"},{"key":"B37","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1016\/j.fsi.2014.05.023","article-title":"Mortality occurrence and pathogen detection in Crassostrea gigas and Mytilus galloprovincialis close-growing in shallow waters (Goro lagoon, Italy)","volume":"41","author":"Domeneghetti","year":"2014","journal-title":"Fish Shellfish Immunol"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2004.10.010","article-title":"Mytilus galloprovincialis filter feeding on the bacterial community in a Mediterranean coastal area (Northern Ionian Sea, Italy)","volume":"39","author":"Stabili","year":"2005","journal-title":"Water Res"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12750","article-title":"Susceptibility of Vibrio aestuarianus 01\/032 to the antibacterial activity of Mytilus haemolymph: identification of a serum opsonin involved in mannose-sensitive interactions","volume":"17","author":"Pezzati","year":"2015","journal-title":"Environ Microbiol"},{"key":"B40","doi-asserted-by":"publisher","first-page":"495","DOI":"10.1007\/s00248-017-1051-6","article-title":"Comparative 16SrDNA gene-based microbiota profiles of the Pacific oyster (Crassostrea gigas) and the Mediterranean mussel (Mytilus galloprovincialis) from a shellfish farm (Ligurian Sea, Italy)","volume":"75","author":"Vezzulli","year":"2018","journal-title":"Microb Ecol"},{"key":"B41","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.aquaculture.2009.03.047","article-title":"Screening for antibacterial and antiviral activities in three bivalve and two gastropod marine molluscs","volume":"293","author":"Defer","year":"2009","journal-title":"Aquaculture"},{"key":"B42","doi-asserted-by":"publisher","first-page":"36\u201440","DOI":"10.1016\/s2221-1691(11)60186-x","article-title":"Antimicrobial activities of the tissue extracts of Babylonia spirata Linnaeus, 1758 (Mollusca: Gastropoda) from Thazhanguda, southeast coast of India","volume":"2","author":"Periyasamy","year":"2012","journal-title":"Asian Pac J Trop Biomed"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1016\/j.jip.2004.02.006","article-title":"Antibacterial activities in various tissues of the horse mussel, Modiolus modiolus","volume":"85","author":"Haug","year":"2004","journal-title":"J Invertebr Pathol"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2014.07.018","article-title":"Comparative study of immune responses in the deep-sea hydrothermal vent mussel Bathymodiolus azoricus and the shallow-water mussel Mytilus galloprovincialis challenged with Vibrio bacteria","volume":"40","author":"Martins","year":"2014","journal-title":"Fish Shellfish Immunol"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2010.10.007","article-title":"Individual sequence variability and functional activities of fibrinogen-related proteins (FREPs) in the Mediterranean mussel (Mytilus galloprovincialis) suggest ancient and complex immune recognition models in invertebrates","volume":"35","author":"Romero","year":"2011","journal-title":"Dev Comp Immunol"},{"key":"B46","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1016\/j.fsi.2016.03.157","article-title":"Multi-species protein similarity clustering reveals novel expanded immune gene families in the eastern oyster Crassostrea virginica","volume":"53","author":"McDowell","year":"2016","journal-title":"Fish Shellfish Immunol"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2011.01.018","article-title":"The C1q domain containing proteins of the Mediterranean mussel Mytilus galloprovincialis: A widespread and diverse family of immune-related molecules","volume":"35","author":"Gerdol","year":"2011","journal-title":"Dev Comp Immunol"},{"key":"B48","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1016\/j.fsi.2015.02.013","article-title":"An updated molecular basis for mussel immunity","volume":"46","author":"Gerdol","year":"2015","journal-title":"Fish Shellfish Immunol"},{"key":"B49","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1007\/978-3-319-76768-0_11","article-title":"Immunity in Molluscs: Recognition and effector mechanisms, with a Focus on Bivalvia","volume-title":"Advances in Comparative Immunology","author":"Gerdol","year":"2018"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2017.12.038","article-title":"The immune response of Mytilus edulis hemocytes exposed to Vibrio splendidus LGP32 strain: A transcriptomic attempt at identifying molecular actors","volume":"74","author":"Tanguy","year":"2018","journal-title":"Fish Shellfish Immunol"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2014.03.021","article-title":"Toll signal transduction pathway in bivalves: Complete cds of intermediate elements and related gene transcription levels in hemocytes of immune stimulated Mytilus galloprovincialis","volume":"45","author":"Toubiana","year":"2014","journal-title":"Dev Comp Immunol"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2013.02.006","article-title":"Toll-like receptors and MyD88 adaptors in Mytilus: Complete cds and gene expression levels","volume":"40","author":"Toubiana","year":"2013","journal-title":"Dev Comp Immunol"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2015.05.001","article-title":"IL-17 signaling components in bivalves: Comparative sequence analysis and involvement in the immune responses","volume":"52","author":"Rosani","year":"2015","journal-title":"Dev Comp Immunol"},{"key":"B54","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1016\/j.dci.2008.02.006","article-title":"Rapid accumulation of an interleukin 17 homolog transcript in Crassostrea gigas hemocytes following bacterial exposure","volume":"32","author":"Roberts","year":"2008","journal-title":"Dev Comp Immunol"},{"key":"B55","doi-asserted-by":"publisher","first-page":"e23481","DOI":"10.7554\/eLife.23481","article-title":"IL17 factors are early regulators in the gut epithelium during inflammatory response to Vibrio in the sea urchin larva","volume":"6","author":"Buckley","year":"2017","journal-title":"eLIFE"},{"key":"B56","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.01644","article-title":"The many and varied roles of tetraspanins in immune cell recruitment and migration","volume":"9","author":"Yeung","year":"2018","journal-title":"Front Immunol"},{"key":"B57","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2017.08.020","article-title":"Molecular identification of disk abalone (Haliotis discus discus) tetraspanin 33 and CD63: Insights into potent players in the disk abalone host defense system","volume":"69","author":"Priyathilaka","year":"2017","journal-title":"Fish Shellfish Immunol"},{"key":"B58","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2014.01.017","article-title":"Deep transcriptome sequencing of Pecten maximus hemocytes: A genomic resource for bivalve immunology","volume":"37","author":"Pauletto","year":"2014","journal-title":"Fish Shellfish Immunol"},{"key":"B59","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2007.03.001","article-title":"Novel transcriptional regulation of the schlafen-2 gene in macrophages in response to TLR-triggered stimulation","volume":"44","author":"Sohn","year":"2007","journal-title":"Mol Immunol"},{"key":"B60","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2015.02.016","article-title":"Syntenin is involved in the bacteria clearance response of kuruma shrimp (Marsupenaeus japonicus)","volume":"44","author":"Liu","year":"2015","journal-title":"Fish Shellfish Immunol"},{"key":"B61","doi-asserted-by":"publisher","DOI":"10.1111\/boc.201500010","article-title":"Syntenin and syndecan in the biogenesis of exosomes","volume":"107","author":"Friand","year":"2015","journal-title":"Biol Cell"},{"key":"B62","doi-asserted-by":"publisher","DOI":"10.7150\/thno.20576","article-title":"Role of extracellular vesicles in viral and bacterial infections: Pathogenesis, diagnostics, and therapeutics","volume":"8","author":"Rodrigues","year":"2018","journal-title":"Theranostics"},{"key":"B63","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines6030079","article-title":"Pathogens and their effect on exosome biogenesis and composition","volume":"6","author":"Jones","year":"2018","journal-title":"Biomed"},{"key":"B64","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2018.08.028","article-title":"TLR crosstalk activates LRP1 to recruit Rab8a and PI3K\u03b3 for suppression of inflammatory responses","volume":"24","author":"Luo","year":"2018","journal-title":"Cell Rep"},{"key":"B65","doi-asserted-by":"publisher","first-page":"304","DOI":"10.1186\/s12974-016-0772-7","article-title":"LRP1 modulates the microglial immune response via regulation of JNK and NF-\u03baB signaling pathways","volume":"13","author":"Yang","year":"2016","journal-title":"J Neuroinflamm"},{"key":"B66","doi-asserted-by":"publisher","DOI":"10.1021\/pr100714b","article-title":"Proteomic analysis of human small cell lung cancer tissues: Up-regulation of coactosin-like protein-1","volume":"10","author":"Jeong","year":"2011","journal-title":"J Proteome Res"},{"key":"B67","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2014.08.012","article-title":"5-Lipoxygenase, a key enzyme for leukotriene biosynthesis in health and disease","volume":"1851","author":"R\u00e5dmark","year":"2015","journal-title":"Biochim Biophys Acta - Mol Cell Biol Lipids"},{"key":"B68","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-5728(01)00438-6","article-title":"Microarray analysis of gene expression in multiple sclerosis and EAE identifies 5-lipoxygenase as a component of inflammatory lesions","volume":"121","author":"Whitney","year":"2001","journal-title":"J Neuroimmunol"},{"key":"B69","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2009.12.022","article-title":"Expression of Mytilus immune genes in response to experimental challenges varied according to the site of collection","volume":"28","author":"Li","year":"2010","journal-title":"Fish Shellfish Immunol"},{"key":"B70","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2006.06.007","article-title":"Specific expression of antimicrobial peptide and HSP70 genes in response to heat-shock and several bacterial challenges in mussels","volume":"22","author":"Cellura","year":"2007","journal-title":"Fish Shellfish Immunol"},{"key":"B71","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2018.07.020","article-title":"Regulation of IL-17 by lncRNA of IRF-2 in the pearl oyster","volume":"81","author":"Huang","year":"2018","journal-title":"Fish Shellfish Immunol"},{"key":"B72","doi-asserted-by":"publisher","DOI":"10.1038\/srep20796","article-title":"Genome-wide identification and characterization of long intergenic noncoding RNAs and their potential association with larval development in the Pacific oyster","volume":"6","author":"Yu","year":"2016","journal-title":"Sci Rep"},{"key":"B73","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2015.11.004","article-title":"Cathepsin L is an immune-related protein in Pacific abalone (Haliotis discus hannai) \u2013 Purification and characterization","volume":"47","author":"Shen","year":"2015","journal-title":"Fish Shellfish Immunol"},{"key":"B74","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2014.07.005","article-title":"Molecular cloning and characterization of cathepsin L from freshwater mussel, Cristaria plicata","volume":"40","author":"Hu","year":"2014","journal-title":"Fish Shellfish Immunol"},{"key":"B75","doi-asserted-by":"publisher","first-page":"728","DOI":"10.1186\/s12864-015-1817-5","article-title":"RNA-Seq in Mytilus galloprovincialis: comparative transcriptomics and expression profiles among different tissues","volume":"16","author":"Moreira","year":"2015","journal-title":"BMC Genomics"},{"key":"B76","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpb.2010.04.009","article-title":"Infection-induced changes in expression of antibacterial and cytokine genes in the gill epithelial cells of Atlantic cod, Gadus morhua during incubation with bacterial pathogens","volume":"156","author":"Caipang","year":"2010","journal-title":"Comp Biochem Physiol Part B Biochem Mol Biol"},{"key":"B77","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-11-559","article-title":"High-throughput sequencing and analysis of the gill tissue transcriptome from the deep-sea hydrothermal vent mussel Bathymodiolus azoricus","volume":"11","author":"Bettencourt","year":"2010","journal-title":"BMC Genomics"}],"container-title":["Frontiers in Immunology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fimmu.2020.615580\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,1,10]],"date-time":"2021-01-10T12:21:09Z","timestamp":1610281269000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fimmu.2020.615580\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12,16]]},"references-count":77,"alternative-id":["10.3389\/fimmu.2020.615580"],"URL":"https:\/\/doi.org\/10.3389\/fimmu.2020.615580","relation":{},"ISSN":["1664-3224"],"issn-type":[{"value":"1664-3224","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,12,16]]},"article-number":"615580"}}