{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T10:16:20Z","timestamp":1792664180689,"version":"4.5.0"},"reference-count":43,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,6,24]],"date-time":"2021-06-24T00:00:00Z","timestamp":1624492800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100015691","name":"Division of Microbiology and Infectious Diseases, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["1R01AI121237-0, 5U19AI089702-01"],"award-info":[{"award-number":["1R01AI121237-0, 5U19AI089702-01"]}],"id":[{"id":"10.13039\/100015691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:p>P48\/45 is a conserved gametocyte antigen involved in <jats:italic>Plasmodium<\/jats:italic> parasite fertilization. A recombinant <jats:italic>Plasmodium vivax<\/jats:italic> P48\/45 (<jats:italic>Pvs<\/jats:italic>48\/45) protein expressed in <jats:italic>Escherichia coli<\/jats:italic> (<jats:italic>E. coli<\/jats:italic>) was highly antigenic and immunogenic in experimental animals and elicited specific transmission-blocking (TB) antibodies in a previous pilot study. Here, a similar <jats:italic>Pvs<\/jats:italic>48\/45 gene was expressed in Chinese Hamster Ovary (CHO) cells and we compared its immunoreactivity with the <jats:italic>E. coli<\/jats:italic> product. Specific antibody titers were determined using plasma from Colombian individuals (n=227) living in endemic areas where both <jats:italic>P. vivax<\/jats:italic> and <jats:italic>P. falciparum<\/jats:italic> are prevalent and from Guatemala (n=54) where <jats:italic>P. vivax<\/jats:italic> is highly prevalent. In Colombia, plasma seroprevalence to CHO-<jats:italic>rPvs<\/jats:italic>48\/45 protein was 46.3%, while for <jats:italic>E. coli<\/jats:italic>-<jats:italic>rPvs<\/jats:italic>48\/45 protein was 36.1% (<jats:italic>p&amp;lt;<\/jats:italic>0.001). In Guatemala, the sero prevalence was 24.1% and 14.8% (<jats:italic>p&amp;lt;<\/jats:italic>0.001), respectively. Reactivity index (RI) against both proteins showed an age-dependent increase. IgG2 was the predominant subclass and the antibody avidity index evaluated by ELISA ranged between 4-6 mol\/L. <jats:italic>Ex vivo P. vivax<\/jats:italic> mosquito direct membrane feeding assays (DMFA) performed in presence of study plasmas, displayed significant parasite transmission-blocking (TB), however, there was no direct correlation between antibody titers and oocysts transmission reduction activity (%TRA). Nevertheless, DMFA with CHO <jats:italic>rPvs<\/jats:italic>48\/45 affinity purified IgG showed a dose response; 90.2% TRA at 100 \u03bcg\/mL and 71.8% inhibition at 10 \u03bcg\/mL. In conclusion, the CHO-r<jats:italic>Pvs<\/jats:italic>48\/45 protein was more immunoreactive in most of the malaria endemic places studied, and CHO-r<jats:italic>Pvs<\/jats:italic>48\/45 specific IgG showed functional activity, supporting further testing of the protein vaccine potential.<\/jats:p>","DOI":"10.3389\/fimmu.2021.634738","type":"journal-article","created":{"date-parts":[[2021,6,24]],"date-time":"2021-06-24T05:24:07Z","timestamp":1624512247000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":10,"title":["Immunoreactivity of Sera From Low to Moderate Malaria-Endemic Areas Against Plasmodium vivax rPvs48\/45 Proteins Produced in Escherichia coli and Chinese Hamster Ovary Systems"],"prefix":"10.3389","volume":"12","author":[{"given":"Myriam","family":"Ar\u00e9valo-Herrera","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazutoyo","family":"Miura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nora","family":"Cespedes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Echeverry","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo","family":"Solano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ang\u00e9lica","family":"Castellanos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan 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