{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T17:10:49Z","timestamp":1793985049599,"version":"4.8.0"},"reference-count":55,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,5,20]],"date-time":"2021-05-20T00:00:00Z","timestamp":1621468800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:p>\n                    Leukocyte cell-derived chemotaxin 2 (LECT2) is a multifunctional cytokine that especially plays an important role in innate immune. However, the roles of LECT2 in the immune response of the economically important fish Nile tilapia (\n                    <jats:italic>Oreochromis niloticus<\/jats:italic>\n                    ) against bacterial infection remains unclear. In this study, a\n                    <jats:italic>lect2<\/jats:italic>\n                    gene from Nile tilapia (\n                    <jats:italic>On-lect2<\/jats:italic>\n                    ) was identified, and its roles in the fish\u2019s immune response against bacterial infection were determined and characterised.\n                    <jats:italic>On-lect2<\/jats:italic>\n                    contains an open reading frame of 456 bp that encodes a peptide of 151 amino acids, as well as the conservative peptidase M23 domain. On-LECT2 is 62%\u201384% identical to other fish species and about 50% identical to mammals. The highest transcriptional level of\n                    <jats:italic>On-lect2<\/jats:italic>\n                    was detected in the liver, whereas the lowest levels were detected in the other tissues. Moreover, the On-LECT2 protein is located mainly in the brain and head kidney. The transcriptional levels of\n                    <jats:italic>On-lect2<\/jats:italic>\n                    substantially increased in the head kidney, brain, liver and spleen after\n                    <jats:italic>Streptococcus agalactiae<\/jats:italic>\n                    infection. Knockdown\n                    <jats:italic>On-lect2<\/jats:italic>\n                    led to higher mortality due to liver necrosis or haemorrhage and splenomegaly.\n                    <jats:italic>In vitro<\/jats:italic>\n                    analysis indicated that the recombinant protein of On-LECT2 improved phagocytic activity of head kidney-derived macrophages.\n                    <jats:italic>In vivo<\/jats:italic>\n                    challenge experiments revealed several functions of On-LECT2 in the immune response of Nile tilapia against bacterial infection, including promotion of inflammation, reduction of tissue damages and improvement of survival rate.\n                  <\/jats:p>","DOI":"10.3389\/fimmu.2021.667781","type":"journal-article","created":{"date-parts":[[2021,5,20]],"date-time":"2021-05-20T12:38:51Z","timestamp":1621514331000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":14,"title":["LECT2 Protects Nile Tilapia (Oreochromis niloticus) Against Streptococcus agalatiae Infection"],"prefix":"10.3389","volume":"12","author":[{"given":"Qi","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiqiang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiqi","family":"Fan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongxiong","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinzhong","family":"Niu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoling","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinchao","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jichang","family":"Jian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2021,5,20]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1016\/0165-2478(96)02572-2","article-title":"Purification 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