{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T20:30:15Z","timestamp":1790281815307,"version":"4.1.0"},"reference-count":47,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,6,18]],"date-time":"2021-06-18T00:00:00Z","timestamp":1623974400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>B-cell non-Hodgkin\u2019s lymphoma (B-NHL) is one of the major complications of primary Sj\u00f6gren\u2019s syndrome (SS). Chronic inflammation and macrophages in SS minor salivary glands have been previously suggested as significant predictors for lymphoma development among SS patients. Lipoprotein-associated phospholipase A2 (Lp-PLA2)\u2014a product mainly of tissue macrophages\u2014is found in the circulation associated with lipoproteins and has been previously involved in cardiovascular, autoimmune, and malignant diseases, including lymphoma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>The purpose of the current study was to investigate the contributory role of Lp-PLA2 in B-NHL development in the setting of primary SS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Lp-PLA2 activity in serum samples collected from 50 primary SS patients with no lymphoma (SS-nL), 9 primary SS patients with lymphoma (SS-L), and 42 healthy controls (HC) was determined by detection of [<jats:sup>3<\/jats:sup>H]PAF degradation products by liquid scintillation counter. Moreover, additional sera from 50 SS-nL, 28 SS-L, and 32 HC were tested for Lp-PLA2 activity using a commercially available ELISA kit. Lp-PLA2 mRNA, and protein expression in minor salivary gland (MSG) tissue samples derived from SS-nL, SS-L patients, and sicca controls (SC) were analyzed by real-time PCR, Western blot, and immunohistochemistry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Serum Lp-PLA2 activity was significantly increased in SS-L compared to both SS-nL and HC by two independent methods implemented [mean \u00b1 SD (nmol\/min\/ml): 62.0 \u00b1 13.4 <jats:italic>vs<\/jats:italic> 47.6 \u00b1 14.4 <jats:italic>vs<\/jats:italic> 50.7 \u00b1 16.6, p-values: 0.003 and 0.04, respectively, and 19.4 \u00b1 4.5 <jats:italic>vs<\/jats:italic> 15.2 \u00b1 3.3 <jats:italic>vs<\/jats:italic> 14.5 \u00b1 3.0, p-values: &amp;lt;0.0001, in both comparisons]. ROC analysis revealed that the serum Lp-PLA2 activity measured either by radioimmunoassay or ELISA has the potential to distinguish between SS-L and SS-nL patients (area under the curve [AUC]: 0.8022, CI [95%]: 0.64\u20130.96, p-value: 0.004 for radioimmunoassay, and AUC: 0.7696, CI [95%]: 0.66\u20130.88, p-value: &amp;lt;0.0001, for ELISA). Lp-PLA2 expression in MSG tissues was also increased in SS-L compared to SS-nL and SC at both mRNA and protein level. ROC analysis revealed that both MSG mRNA and protein Lp-PLA2 have the potential to distinguish between SS-nL and SS-L patients (area under the curve [AUC] values of 0.8490, CI [95%]: 0.71\u20130.99, p-value: 0.0019 and 0.9444, CI [95%]: 0.79\u20131.00, p- value: 0.0389 respectively). No significant difference in either serum Lp-PLA2 activity or MSG tissue expression was observed between SS-nL and HC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Lp-PLA2 serum activity and MSG tissue mRNA\/protein expression could be a new biomarker and possibly a novel therapeutic target for B-cell lymphoproliferation in the setting of SS.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fimmu.2021.683623","type":"journal-article","created":{"date-parts":[[2021,6,18]],"date-time":"2021-06-18T17:09:04Z","timestamp":1624036144000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":18,"title":["Lipoprotein-Associated Phospholipase A2: A Novel Contributor in Sj\u00f6gren\u2019s Syndrome-Related 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