{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T16:01:21Z","timestamp":1753891281709,"version":"3.41.2"},"reference-count":60,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,11,22]],"date-time":"2021-11-22T00:00:00Z","timestamp":1637539200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009590","name":"Gansu Education Department","doi-asserted-by":"publisher","award":["GSSYLXM-02"],"award-info":[{"award-number":["GSSYLXM-02"]}],"id":[{"id":"10.13039\/501100009590","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009590","name":"Gansu Education Department","doi-asserted-by":"publisher","award":["2021CXZX-351"],"award-info":[{"award-number":["2021CXZX-351"]}],"id":[{"id":"10.13039\/501100009590","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31960646"],"award-info":[{"award-number":["31960646"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009590","name":"Gansu Education Department","doi-asserted-by":"publisher","award":["GARS-ZJ-1"],"award-info":[{"award-number":["GARS-ZJ-1"]}],"id":[{"id":"10.13039\/501100009590","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>The n6-methyladenosine (m6A) modification is present widely in mRNAs and long non-coding RNAs (lncRNAs), and is related to the occurrence and development of certain diseases. However, the role of m6A methylation in <jats:italic>Clostridium perfringens type C<\/jats:italic> infectious diarrhea remains unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Here, we treated intestinal porcine jejunum epithelial cells (IPEC-J2 cells) with <jats:italic>Clostridium perfringens<\/jats:italic> beta2 (CPB2) toxin to construct an <jats:italic>in vitro<\/jats:italic> model of <jats:italic>Clostridium perfringens type C (C. perfringens type C)<\/jats:italic> infectious diarrhea, and then used methylated RNA immunoprecipitation sequencing (MeRIP-seq) and RNA sequencing (RNA-seq) to identify the methylation profiles of mRNAs and lncRNAs in IPEC-J2 cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We identified 6,413 peaks, representing 5,825 m6A-modified mRNAs and 433 modified lncRNAs, of which 4,356 m6A modified mRNAs and 221 m6A modified lncRNAs were significantly differential expressed between the control group and CPB2 group. The motif GGACU was enriched significantly in both the control group and the CPB2 group. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation analysis showed that the differentially methylated modified mRNAs were mainly enriched in Hippo signaling pathway and Wnt signaling pathway. In addition, the target genes of the differentially m6A modified lncRNAs were related to defense response to virus and immune response. For example, <jats:italic>ENSSSCG00000042575<\/jats:italic>, <jats:italic>ENSSSCG00000048701<\/jats:italic> and <jats:italic>ENSSSCG00000048785<\/jats:italic> might regulate the defense response to virus, immune and inflammatory response to resist the harmful effects of viruses on cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In summary, this study established the m6A transcription profile of mRNAs and lncRNAs in IPEC-J2 cells treated by CPB2 toxin. Further analysis showed that m6A-modified RNAs were related to defense against viruses and immune response after CPB2 toxin treatment of the cells. Threem6A-modified lncRNAs, <jats:italic>ENSSSCG00000042575<\/jats:italic>, <jats:italic>ENSSSCG00000048785<\/jats:italic> and <jats:italic>ENSSSCG00000048701<\/jats:italic>, were most likely to play a key role in CPB2 toxin-treated IPEC-J2 cells. The results provide a theoretical basis for further research on the role of m6A modification in piglet diarrhea.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fimmu.2021.769204","type":"journal-article","created":{"date-parts":[[2021,11,22]],"date-time":"2021-11-22T07:26:46Z","timestamp":1637566006000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":8,"title":["N6-Methyladenosine Methylation Analysis of Long Noncoding RNAs and mRNAs in IPEC-J2 Cells Treated With Clostridium perfringens beta2 Toxin"],"prefix":"10.3389","volume":"12","author":[{"given":"Jiaojiao","family":"Yang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiaoli","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juanli","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoli","family":"Gao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruirui","family":"Luo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaihui","family":"Xie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyu","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zunqiang","family":"Yan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pengfei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuangbao","family":"Gun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2021,11,22]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1590\/0103-8478cr20140927","article-title":"Clostridium Perfringens: A Review of the Disease in Pigs, Horses and Broiler Chickens","volume":"45","author":"Silva","year":"2015","journal-title":"Ciec Rural"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.8.3584-3591.2003","article-title":"Genotyping and Phenotyping of Beta2-Toxigenic Clostridium Perfringens Fecal Isolates Associated With Gastrointestinal Diseases in Piglets","volume":"41","author":"Waters","year":"2003","journal-title":"J Clin Microbiol"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.22489","article-title":"Peptidoglycan Induces Interleukin-6 Expression Through the TLR2 Receptor, JNK, C-Jun, and AP-1 Pathways in Microglia","volume":"226","author":"Lin","year":"2011","journal-title":"J Cell Physiol"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00579-13","article-title":"The P38 MAPK and JNK Pathways Protect Host Cells Against Clostridium Perfringens Beta-Toxin","volume":"81","author":"Nagahama","year":"2013","journal-title":"Infect Immunity"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab517","article-title":"Epitranscriptomic Editing of the RNA N6-Methyladenosine Modification by Dcasrx Conjugated Methyltransferase and Demethylase","volume":"49","author":"Xia","year":"2021","journal-title":"Nucleic Acids Res"},{"key":"B6","doi-asserted-by":"publisher","first-page":"293","DOI":"10.1038\/nrg3724","article-title":"Gene Expression Regulation Mediated Through Reversible m6A RNA Methylation","volume":"15","author":"Fu","year":"2014","journal-title":"Nat Rev Genet"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.3390\/epigenomes4030016","article-title":"Implication of m6A mRNA Methylation in Susceptibility to Inflammatory Bowel Disease","volume":"4","author":"Sebastian-Delacruz","year":"2020","journal-title":"Epigenomes"},{"key":"B8","doi-asserted-by":"publisher","first-page":"eaba0647","DOI":"10.1126\/sciadv.aba0647","article-title":"Interplay of m6A and H3K27 Trimethylation Restrains Inflammation During Bacterial Infection","volume":"6","author":"Wu","year":"2020","journal-title":"Sci Advances"},{"key":"B9","doi-asserted-by":"publisher","first-page":"1146","DOI":"10.1016\/j.celrep.2018.10.020","article-title":"N6-Methyladenosine Inhibits Local Ribonucleolytic Cleavage to Stabilize mRNAs in Arabidopsis","volume":"25","author":"Anderson","year":"2018","journal-title":"Cell Rep"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1080\/15476286.2016.1201628","article-title":"m6A: Signaling for mRNA Splicing","volume":"13","author":"Adhikari","year":"2016","journal-title":"RNA Biol"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2017.15","article-title":"YTHDF3 Facilitates Translation and Decay of N6-Methyladenosine-Modified RNA","volume":"27","author":"Shi","year":"2017","journal-title":"Cell Res"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.03.031","article-title":"Transcription Impacts the Efficiency of mRNA Translation via Co-Transcriptional N6-Adenosine Methylation","volume":"169","author":"Slobodin","year":"2017","journal-title":"Cell"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.3390\/genes10010035","article-title":"Methods for RNA Modification Mapping Using Deep Sequencing: Established and New Emerging Technologies","volume":"10","author":"Motorin","year":"2019","journal-title":"Genes"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-020-07192-8","article-title":"Integrated Analysis of lncRNAs and mRNAs Reveals Key Trans-Target Genes Associated With ETEC-F4ac Adhesion Phenotype in Porcine Small Intestine Epithelial Cells","volume":"21","author":"Augustino","year":"2020","journal-title":"BMC Genomics"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.05.075","article-title":"A Long Noncoding RNA lincRNA-EPS Acts as a Transcriptional Brake to Restrain Inflammation","volume":"165","author":"Atianand","year":"2016","journal-title":"Cell"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2019.00130","article-title":"Integrative Analyses of Long Non-Coding RNA and mRNA Involved in Piglet Ileum Immune Response to Clostridium Perfringens Type C Infection","volume":"9","author":"Huang","year":"2019","journal-title":"Front Cell Infect Microbiol"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2020.104379","article-title":"Effects of Clostridium Perfringens Beta2 Toxin on Apoptosis, Inflammation, and Barrier Function of Intestinal Porcine Jejunum Epithelial Cells","volume":"147","author":"Gao","year":"2020","journal-title":"Microb Pathogen"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2020.144999","article-title":"Clostridium Perfringens Beta2 Toxin Induced In Vitro Oxidative Damage and its Toxic Assessment in Porcine Small Intestinal Epithelial Cell Lines","volume":"759","author":"Rl","year":"2020","journal-title":"Gene"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty560","article-title":"Fastp: An Ultra-Fast All-in-One FASTQ Preprocessor","volume":"34","author":"Chen","year":"2018","journal-title":"Bioinformatics"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3317","article-title":"HISAT: A Fast Spliced Aligner With Low Memory Requirements","volume":"12","author":"Kim","year":"2015","journal-title":"Nat Methods"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2014.06.008","article-title":"A Protocol for RNA Methylation Differential Analysis With MeRIP-Seq Data and Exomepeak R\/Bioconductor Package","volume":"69","author":"Meng","year":"2014","journal-title":"Methods"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp335","article-title":"MEME Suite: Tools for Motif Discovery and Searching","volume":"37","author":"Bailey","year":"2009","journal-title":"Nucleic Acids Res"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv145","article-title":"ChIPseeker: An R\/Bioconductor Package for ChIP Peak Annotation, Comparison and Visualization","volume":"31","author":"Yu","year":"2015","journal-title":"Bioinformatics"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2010.05.004","article-title":"Simple Combinations of Lineage-Determining Transcription Factors Prime Cis-Regulatory Elements Required for Macrophage and B Cell Identities - ScienceDirect","volume":"38","author":"Heinz","year":"2010","journal-title":"Mol Cell"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3122","article-title":"StringTie Enables Improved Reconstruction of a Transcriptome From RNA-Seq Reads","volume":"33","author":"Pertea","year":"2015","journal-title":"Nat Biotechnol"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1621","article-title":"Transcript Assembly and Quantification by RNA-Seq Reveals Unannotated Transcripts and Isoform Switching During Cell Differentiation","volume":"28","author":"Trapnell","year":"2010","journal-title":"Nat Biotechnol"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm391","article-title":"CPC: Assess the Protein-Coding Potential of Transcripts Using Sequence Features and Support Vector Machine","volume":"35","author":"Kong","year":"2007","journal-title":"Nucleic Acids Res"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt646","article-title":"Utilizing Sequence Intrinsic Composition to Classify Protein-Coding and Long non-Coding Transcripts","volume":"41","author":"Sun","year":"2013","journal-title":"Nucleic Acids Res"},{"key":"B29","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1093\/bioinformatics\/btp616","article-title":"Edger: A Bioconductor Package for Differential Expression Analysis of Digital Gene Expression Data","volume":"26","author":"Smyth","year":"2010","journal-title":"Bioinformatics"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.2428","article-title":"Cis-Acting Noncoding RNAs: Friends and Foes","volume":"19","author":"Guil","year":"2012","journal-title":"Nat Struct Mol Biol"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts519","article-title":"RIsearch: Fast RNA-RNA Interaction Search Using a Simplified Nearest-Neighbor Energy Model","volume":"28","author":"Wenzel","year":"2012","journal-title":"Bioinf (Oxford England)"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm882","article-title":"KEGG for Linking Genomes to Life and the Environment","volume":"36","author":"Minoru","year":"2008","journal-title":"Nucleic Acids Res"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2010-11-2-r14","article-title":"Gene Ontology Analysis for RNA-Seq: Accounting for Selection Bias","volume":"11","author":"Young","year":"2010","journal-title":"Genome Biol"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001","article-title":"Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2 \u0394\u0394 C T Method","volume":"25","author":"KJL","year":"2001","journal-title":"Methods"},{"key":"B35","doi-asserted-by":"publisher","first-page":"399","DOI":"10.1016\/j.vetmic.2009.07.003","article-title":"Clostridia as Agents of Zoonotic Disease","volume":"140","author":"Songer","year":"2010","journal-title":"Vet Microbiol"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04573.x","article-title":"Association of Beta2 Toxin Production With Clostridium Perfringens Type A Human Gastrointestinal Disease Isolates Carrying a Plasmid Enterotoxin Gene","volume":"56","author":"Fisher","year":"2010","journal-title":"Mol Microbiol"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.22041","article-title":"Reduced M 6 A mRNA Methylation is Correlated With the Progression of Human Cervical Cancer","volume":"8","author":"Wang","year":"2017","journal-title":"Oncotarget"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtn.2020.02.002","article-title":"N6-Methyladenosine METTL3 Modulates the Proliferation and Apoptosis of Lens Epithelial Cells in Diabetic Cataract","volume":"20","author":"Yang","year":"2020","journal-title":"Mol Therapy Nucleic Acids"},{"key":"B39","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1002\/lipd.12023","article-title":"Curcumin Attenuates Lipopolysaccharide-Induced Hepatic Lipid Metabolism Disorder by Modification of m6A RNA Methylation in Piglets","volume":"53","author":"Lu","year":"2018","journal-title":"Lipids"},{"key":"B40","doi-asserted-by":"publisher","first-page":"9750","DOI":"10.1016\/j.ygeno.2020.09.038","article-title":"m6A mRNA Methylation Analysis Provides Novel Insights Into Heat Stress Responses in the Liver Tissue of Sheep","volume":"9","author":"Lu","year":"2020","journal-title":"Genomics"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-11232-7","article-title":"m6A Modification of a 3\u2019 UTR Site Reduces RME1 mRNA Levels to Promote Meiosis","volume":"10","author":"Bushkin","year":"2019","journal-title":"Nat Commun"},{"key":"B42","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1038\/s41421-018-0019-0","article-title":"VIRMA Mediates Preferential mA mRNA Methylation in 3 UTR and Near Stop Codon and Associates With Alternative Polyadenylation","volume":"4","author":"Yue","year":"2018","journal-title":"Cell Discov"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2016.04.002","article-title":"Posttranscriptional M(6)A Editing of HIV-1 mRNAs Enhances Viral Gene Expression","volume":"4","author":"Kennedy","year":"2016","journal-title":"Cell Host Microbe"},{"key":"B44","doi-asserted-by":"publisher","first-page":"1004","DOI":"10.1038\/s41593-018-0173-6","article-title":"Synaptic N 6 -Methyladenosine (M 6 A) Epitranscriptome Reveals Functional Partitioning of Localized Transcripts","volume":"21","author":"Merkurjev","year":"2018","journal-title":"Nat Neurosci"},{"key":"B45","doi-asserted-by":"publisher","first-page":"2995","DOI":"10.1105\/tpc.16.00912","article-title":"ALKBH10B is An RNA N6-Methyladenosine Demethylase Affecting Arabidopsis Floral Transition","volume":"29","author":"Duan","year":"2017","journal-title":"Plant Cell"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/18.19.5735","article-title":"Sequence Specificity of the Human mRNA N6-Adenosine Methylase In Vitro","volume":"18","author":"Harper","year":"1990","journal-title":"Nucleic Acids Res"},{"key":"B47","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41422-018-0113-8","article-title":"A Novel m6A Reader Prrc2a Controls Oligodendroglial Specification and Myelination","volume":"29","author":"Rong","year":"2019","journal-title":"Cell Res"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1016\/j.critrevonc.2015.12.005","article-title":"The Wnt Signaling Pathway in Cancer","volume":"12","author":"Duchartre","year":"2016","journal-title":"Crit Rev Oncol"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2019.00211","article-title":"The Hippo Signaling Pathway in Cardiac Development and Diseases","volume":"7","author":"Mia","year":"2019","journal-title":"Front Cell Dev Biol"},{"key":"B50","doi-asserted-by":"publisher","first-page":"1923","DOI":"10.1016\/j.celrep.2019.10.009","article-title":"The GTPase Domain of MX2 Interacts With the HIV-1 Capsid, Enabling Its Short Isoform to Moderate Antiviral Restriction","volume":"29","author":"Betancor","year":"2019","journal-title":"Cell Rep"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2019.12.009","article-title":"Interferon-Inducible MX2 is a Host Restriction Factor of Hepatitis B Virus Replication","volume":"72","author":"Wang","year":"2019","journal-title":"J Hepatol"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.01432","article-title":"Quantitative Proteomic Profile of Psoriatic Epidermis Identifies OAS2 as a Novel Biomarker for Disease Activity","volume":"11","author":"Zhou","year":"2020","journal-title":"Front Immunol"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.00889-17","article-title":"Ifit2 Is a Restriction Factor in Rabies Virus Pathogenicity","author":"Davis","year":"2017","journal-title":"J Virol"},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.1038\/nature07924","article-title":"Zc3h12a is an RNase Essential for Controlling Immune Responses by Regulating mRNA Decay","volume":"458","author":"Matsushita","year":"2009","journal-title":"Nature"},{"key":"B55","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.13491","article-title":"METTL3 Regulates Alternative Splicing of MyD88 Upon the Lipopolysaccharide-Induced Inflammatory Response in Human Dental Pulp Cells","volume":"22","author":"Feng","year":"2018","journal-title":"J Cell Mol Med"},{"key":"B56","doi-asserted-by":"publisher","DOI":"10.1038\/nature23450","article-title":"m6A mRNA Methylation Controls T Cell Homeostasis by Targeting the IL-7\/STAT5\/SOCS Pathways","volume":"548","author":"Li","year":"2017","journal-title":"Nature"},{"key":"B57","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3830","article-title":"The RNA Helicase DDX46 Inhibits Innate Immunity by Entrapping m6A-Demethylated Antiviral Transcripts in the Nucleus","volume":"18","author":"Zheng","year":"2017","journal-title":"Nat Immunol"},{"key":"B58","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.00922","article-title":"A Review in Research Progress Concerning m6A Methylation and Immunoregulation","volume":"10","author":"Zhang","year":"2019","journal-title":"Front Immunol"},{"key":"B59","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1007\/BF03257159","article-title":"The Role of Interleukin-8 and its Receptors in Inflammatory Lung Disease: Implications for Therapy","volume":"1","author":"Pease","year":"2002","journal-title":"Am J Respir Med Drugs Devices Other Interventions"},{"key":"B60","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2020.563693","article-title":"LncRNA MALAT1 Affects Mycoplasma Pneumoniae Pneumonia via NF-\u03baB Regulation","volume":"8","author":"Gu","year":"2020","journal-title":"Front Cell Dev Biol"}],"container-title":["Frontiers in Immunology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fimmu.2021.769204\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,22]],"date-time":"2021-11-22T07:26:51Z","timestamp":1637566011000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fimmu.2021.769204\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,22]]},"references-count":60,"alternative-id":["10.3389\/fimmu.2021.769204"],"URL":"https:\/\/doi.org\/10.3389\/fimmu.2021.769204","relation":{},"ISSN":["1664-3224"],"issn-type":[{"type":"electronic","value":"1664-3224"}],"subject":[],"published":{"date-parts":[[2021,11,22]]},"article-number":"769204"}}