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Immunol."],"abstract":"<jats:p>Marek\u2019s disease virus (MDV), the etiologic agent for Marek\u2019s disease (MD), causes a deadly lymphoproliferative disease in chickens. Causes of the well-documented association between genetically defined lines of chicken and resistance to MD remain unknown. Here, the frequencies of IFN-gamma producing <jats:italic>pp38<\/jats:italic> and <jats:italic>MEQ<\/jats:italic>-specific T cell responses were determined in line N (B21 haplotype; MD-resistant) and line P2a (B19 haplotype, MD-susceptible) chickens after infection with vaccine and\/or virulent (RB1B) strains of MDV using both standard <jats:italic>ex vivo<\/jats:italic> and cultured chIFN-gamma ELISPOT assays. Notably, MDV infection of na\u00efve and vaccinated MD-resistant chickens induced higher frequencies of IFN-gamma producing MDV-specific T cell responses using the cultured and <jats:italic>ex vivo<\/jats:italic> ELISPOT assay, respectively. Remarkably, vaccination did not induce or boost <jats:italic>MEQ<\/jats:italic>-specific effector T cells in the susceptible chickens, while it boosted both <jats:italic>pp38<\/jats:italic>-and <jats:italic>MEQ<\/jats:italic>-specific response in resistant line. Taken together, our results revealed that there is a direct association between the magnitude of T cell responses to <jats:italic>pp38<\/jats:italic> and <jats:italic>MEQ<\/jats:italic> of MDV antigens and resistance to the disease.<\/jats:p>","DOI":"10.3389\/fimmu.2021.784359","type":"journal-article","created":{"date-parts":[[2022,1,13]],"date-time":"2022-01-13T05:00:35Z","timestamp":1642050035000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":16,"title":["Differential Virus-Specific IFN-Gamma Producing T Cell Responses to Marek\u2019s Disease Virus in Chickens With B19 and B21 MHC Haplotypes"],"prefix":"10.3389","volume":"12","author":[{"given":"Nitish","family":"Boodhoo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shahriar","family":"Behboudi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,1,13]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1186\/s13567-016-0404-3","article-title":"Marek\u2019s Disease in Chickens: A Review With Focus on Immunology","volume":"47","author":"Boodhoo","year":"2016","journal-title":"Vet Res"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1382","article-title":"Marek\u2019s Disease Virus: From Miasma to Model","volume":"4","author":"Osterrieder","year":"2006","journal-title":"Nat Rev Microbiol"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1637\/11841-041318-Review.1","article-title":"Virus-Induced 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