{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T17:10:35Z","timestamp":1792429835268,"version":"4.5.0"},"reference-count":67,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,1,27]],"date-time":"2022-01-27T00:00:00Z","timestamp":1643241600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:p>MicroRNAs (miRNAs) are important signaling regulators that are involved in regulating the innate immunity of crustacean. However, few studies focus on the role of crustacean miRNAs in the cellular immunity have been reported. In this study, we showed that the expression of miR-133 was significantly up-regulated in the mud crab <jats:italic>Scylla paramamosain<\/jats:italic> after infection by white spot syndrome virus (WSSV) or <jats:italic>Vibrio parahaemolyticus<\/jats:italic>. The anti-miRNA oligonucleotide AMO-miR-133 was used to knock down miR-133 expression in <jats:italic>S. paramamosain<\/jats:italic>. The number of WSSV copies increased significantly in WSSV-infected crabs after miR-133 knockdown. Knockdown of miR-133 also enhanced the mortality rates of WSSV-infected and <jats:italic>V. parahaemolyticus-<\/jats:italic>infected mud crabs, and it significantly enhanced the expression of the astakine, which was confirmed by real-time quantitative PCR and western blot analysis. The data also indicate that miR-133 may affect hemocyte proliferation in <jats:italic>S. paramamosain<\/jats:italic> by regulating <jats:italic>astaki<\/jats:italic>ne expression. miR-133 Knockdown enhanced the apoptosis or phagocytosis of crab hemocytes, and increased the mortality of mud crabs after WSSV or <jats:italic>V. parahaemolyticus<\/jats:italic> infection. These results indicate that miR-133 is involved in the host immune response to WSSV and <jats:italic>V. parahaemolyticus<\/jats:italic> infection in mud crabs. Taken together, our research provides new insights for the control of viral or vibrio diseases in <jats:italic>S. paramamosain.<\/jats:italic><\/jats:p>","DOI":"10.3389\/fimmu.2021.812717","type":"journal-article","created":{"date-parts":[[2022,1,27]],"date-time":"2022-01-27T11:27:12Z","timestamp":1643282832000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":15,"title":["The Role of microRNA-133 in Hemocyte Proliferation and Innate Immunity of Scylla paramamosain"],"prefix":"10.3389","volume":"12","author":[{"given":"Yunfei","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongyong","family":"Lai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiujuan","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,1,27]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1007\/s12038-011-9083-4","article-title":"MicroRNA Functional Network in Pancreatic Cancer: From Biology to Biomarker of Disease","volume":"36","author":"Wang","year":"2011","journal-title":"J Biosci"},{"key":"B2","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1038\/s41580-018-0059-1","article-title":"Regulation of microRNA Biogenesis and Its Crosstalk With Other Cellular Pathways","volume":"20","author":"Treiber","year":"2019","journal-title":"Nat Rev Mol Cell Biol"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.01.002","article-title":"MicroRNAs: Target Recognition and Regulatory Functions","volume":"136","author":"Bartel","year":"2009","journal-title":"Cell"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky1141","article-title":"Mirbase: From microRNA Sequences to Function","volume":"47","author":"Kozomara","year":"2019","journal-title":"Nucleic Acids Res"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0073392","article-title":"Characterization of microRNAs in Mud Crab Scylla Paramamosain Under Vibrio Parahaemolyticus Infection","volume":"8","author":"Li","year":"2013","journal-title":"PloS One"},{"key":"B6","doi-asserted-by":"publisher","first-page":"335","DOI":"10.1186\/s12864-018-4728-4","article-title":"Acute Hepato\u2043Pancreatic Necrosis Disease (AHPND) Related microRNAs in Litopenaeus Vannamei Infected With AHPND\u2043causing Strain of Vibrio Parahemolyticus","volume":"19","author":"Zheng","year":"2018","journal-title":"BMC Genomics"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2015.10.012","article-title":"Differential Expression of microRNAs in Shrimp Marsupenaeus Japonicus in Response to Vibrio Alginolyticus Infection","volume":"55","author":"Zhu","year":"2016","journal-title":"Dev Comp Immunol"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2015.10.012","article-title":"Eriocheir Sinensis microRNA-7 Targets Crab Myd88 to Enhance White Spot Syndrome Virus Replication","volume":"79","author":"Huang","year":"2018","journal-title":"Fish Shellfish Immunol"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2017.00164","article-title":"Host microRNA-217 Promotes White Spot Syndrome Virus Infection by Targeting Tube in the Chinese Mitten Crab (Eriocheir Sinensis)","volume":"7","author":"Huang","year":"2017","journal-title":"Front Cell Infection Microbiol"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2017.05.060","article-title":"The Identification of microRNAs Involved in the Response of Chinese Shrimp Fenneropenaeus Chinensis to White Spot Syndrome Virus Infection","volume":"68","author":"Li","year":"2017","journal-title":"Fish Shellfish Immunol"},{"key":"B11","doi-asserted-by":"publisher","first-page":"3720","DOI":"10.1038\/s41598-020-60685-0","article-title":"Temperature Potentially Induced Distinctive Flavor of Mud Crab Scylla Paramamosain Mediated by Gut Microbiota","volume":"10","author":"Tang","year":"2020","journal-title":"Sci Rep"},{"key":"B12","volume-title":"F.B.O.T. 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