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Immunol."],"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>In order to determine whether the immune balance of T helper 17(Th17)\/regulatory T(Treg) is related to the pathogenesis of idiopathic retroperitoneal fibrosis (IRPF), we analyzed the differences in peripheral blood lymphocytes, CD4+T cell subsets and cytokines between patients with IRPF and healthy people to clarify the CD4+T cell subsets, especially Treg cell subsets, and the role of cytokines in the pathogenesis of IRPF.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This study included 22 patients with IRPF, 36 patients with IgG4-related diseases (IgG4-RD) without retroperitoneal fibrosis (RPF), and 28 healthy controls. The absolute numbers and percentage of peripheral blood lymphocyte subsets and CD4+T cell subsets in each group were detected by flow cytometry, and the serum cytokine level was detected by flow cytometric bead array (CBA).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Compared with the healthy group, the absolute value of B cells in peripheral blood of IRPF patients was significantly decreased, and T, natural killer (NK), CD4+ and CD8+ were not significantly abnormal. The absolute numbers of Th2 cells were lower than healthy group(p=0.043). In particular, the absolute numbers of Treg cells were significantly lower than healthy group(p&amp;lt;0.001), while the absolute numbers of Th17 cells increased(p=0.682). Th17\/Treg was significantly higher than healthy group (p&amp;lt; 0.001). Cytokine analysis showed that the level of interleukin (IL)-4 in IRPF patients was higher than healthy group(p=0.011), IL-6, IL-10, IL-17, TNF-\u03b1 and IFN-\u03b3 were significantly higher than healthy group (all p&amp;lt;0.001). Receiver operating characteristic (ROC) curves showed that IL-10 and TNF-\u03b1 could distinguish bilateral ureteral dilatation in IRPF patients, with areas under the ROC curve (AUCs) of 0.813 (95% CI:0.607-1.000, p=0.026) and 0.950 (95% CI:0.856-1.000, p=0.001), respectively. IL-6 could distinguish bilateral ureteral obstruction, with an AUC of 0.861 (95% CI: 0.682-1.000, p=0.015).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our study showed that IRPF patients had reduced Treg cells and indeed had Th17\/Treg imbalance, which may be related to the pathogenesis of the disease. The levels of IL-6, IL-10 and TNF-\u03b1 appear to be associated with the progression of IRPF.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fimmu.2022.1012513","type":"journal-article","created":{"date-parts":[[2022,11,29]],"date-time":"2022-11-29T05:50:25Z","timestamp":1669701025000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Decreased absolute number of peripheral regulatory T cells in patients with idiopathic retroperitoneal 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