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Immunol."],"abstract":"<jats:p>Latent tuberculosis infection (LTBI) treatment is known to accelerate the decline in TB incidence, especially in high-risk populations. <jats:italic>Mycobacterium tuberculosis<\/jats:italic> (<jats:italic>M. tb<\/jats:italic>) expression profiles differ at different growth periods, and vaccines protective and therapeutic effects may increase when they include antigenic compositions from different periods. To develop a post-exposure vaccine that targets LTBI, we constructed four therapeutic DNA vaccines (<jats:italic>A39<\/jats:italic>, <jats:italic>B37<\/jats:italic>, <jats:italic>B31<\/jats:italic>, and <jats:italic>B21<\/jats:italic>) using different combinations of antigens from the proliferation phase (Ag85A, Ag85B), PE\/PPE family (Rv3425), and latent phase (Rv2029c, Rv1813c, Rv1738). We compared the immunogenicity of the four DNA vaccines in C57BL\/6j mice. The <jats:italic>B21<\/jats:italic> vaccine stimulated the strongest cellular immune responses, namely Th1\/Th17 and CD8<jats:sup>+<\/jats:sup> cytotoxic T lymphocyte responses. It also induced the generation of strengthened effector memory and central memory T cells. In latently infected mice, the <jats:italic>B21<\/jats:italic> vaccine significantly reduced bacterial loads in the spleens and lungs and decreased lung pathology. In conclusion, the <jats:italic>B21<\/jats:italic> DNA vaccine can enhance T cell responses and control the reactivation of LTBI.<\/jats:p>","DOI":"10.3389\/fimmu.2022.1025931","type":"journal-article","created":{"date-parts":[[2022,12,7]],"date-time":"2022-12-07T06:31:25Z","timestamp":1670394685000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":15,"title":["B21 DNA vaccine expressing ag85b, rv2029c, and rv1738 confers a robust therapeutic effect against latent Mycobacterium tuberculosis 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