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Immunol."],"abstract":"<jats:p>Alveolar echinococcosis (AE) is a parasitic disease caused by <jats:italic>E. multilocularis<\/jats:italic> metacestodes and it is highly prevalent in the northern hemisphere. We have previously found that vaccination with <jats:italic>E. multilocularis<\/jats:italic> Leucine aminopeptidase (EM-LAP) induced specific immune response and had an inhibiting effect on the parasites. In this study, the therapeutic effect of recombinant EM-LAP (rEM-LAP) on AE was evaluated and verified using Ubenimex, a broad-spectrum inhibitor of LAP. The results reveal that rEM-LAP could inhibit cyst growth and invasion and induce specific immunity response in BALB\/c mice infected with <jats:italic>E. multilocularis<\/jats:italic> protoscoleces. The ultrasonic, MRI, and morphological results show that treatment with rEM-LAP inhibits <jats:italic>E. multilocularis<\/jats:italic> infection and reduces cyst weight, number, fibrosis and invasion. The same effect is observed for the treatment with Ubenimex by inhibiting LAP activity. The indirect ELISA shows that rEM-LAP could induce specific immunity response and produce high levels of IgG, IgG1, IgG2a, IgM, and IgA, and the serum levels of IFN-\u03b3 and IL-4 are significantly increased compared to the control groups, indicating that treatment with rEM-LAP leads to a Th1 and Th2 mixed-type immune response. This study suggests that EM-LAP could be a potential therapeutic target of <jats:italic>E. multilocularis<\/jats:italic> infection.<\/jats:p>","DOI":"10.3389\/fimmu.2022.1027500","type":"journal-article","created":{"date-parts":[[2022,10,14]],"date-time":"2022-10-14T09:27:40Z","timestamp":1665739660000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":5,"title":["Therapeutic effect on Alveolar echinococcosis by targeting EM-Leucine 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