{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:16:33Z","timestamp":1787026593811,"version":"build-2736575974"},"reference-count":41,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,3,15]],"date-time":"2022-03-15T00:00:00Z","timestamp":1647302400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:p>\n                    <jats:italic>Trueperella pyogenes<\/jats:italic>\n                    (\n                    <jats:italic>T. pyogenes<\/jats:italic>\n                    ) is a commensal and an opportunistic pathogen of animals. This organism can cause inflammatory diseases, such as pneumonia, mastitis and endometritis in hosts. However, the molecular basis for the pro-inflammatory properties of this organism is still largely unknown. In the current study, using murine macrophages as model, the ability of\n                    <jats:italic>T. pyogenes<\/jats:italic>\n                    to induce pyroptosis was first determined. Then, pyolysin (PLO), a cholesterol-dependent cytolysin secreted by\n                    <jats:italic>T. pyogenes<\/jats:italic>\n                    , was found to be closely related to\n                    <jats:italic>T. pyogenes<\/jats:italic>\n                    -induced pyroptosis. Next, our work showed that PLO can form pores in the cell membrane, leading to the efflux of potassium (K\n                    <jats:sup>+<\/jats:sup>\n                    ), NOD-like receptor family pyrin domain containing 3 (NLRP3) inflammasome-mediated caspase-1 activation, and gasdermin D (GSDMD) cleavage. Inhibition of the K\n                    <jats:sup>+<\/jats:sup>\n                    \/NLRP3\/caspase-1\/GSDMD pathway abolished\n                    <jats:italic>T. pyogenes<\/jats:italic>\n                    and PLO-induced IL-1\u03b2 release. Taken together, these results indicate\n                    <jats:italic>T. pyogenes<\/jats:italic>\n                    -induced inflammation is related to PLO-induced pyroptosis and IL-1\u03b2 release. Our work shed light on the pathogenesis of\n                    <jats:italic>T. pyogenes<\/jats:italic>\n                    and the interaction between\n                    <jats:italic>T. pyogenes<\/jats:italic>\n                    and hosts\u2019 immune system.\n                  <\/jats:p>","DOI":"10.3389\/fimmu.2022.832458","type":"journal-article","created":{"date-parts":[[2022,3,15]],"date-time":"2022-03-15T04:53:47Z","timestamp":1647320027000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":25,"title":["Pyolysin of Trueperella pyogenes Induces Pyroptosis and IL-1\u03b2 Release in Murine Macrophages Through Potassium\/NLRP3\/Caspase-1\/Gasdermin D Pathway"],"prefix":"10.3389","volume":"13","author":[{"given":"Hongmin","family":"Liang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junwei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenlong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,3,15]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"2737","DOI":"10.3390\/ijms20112737","article-title":"Pathogenicity and Virulence of Trueperella Pyogenes: A Review","volume":"20","author":"Rzewuska","year":"2019","journal-title":"Int J 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