{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T09:38:48Z","timestamp":1782898728040,"version":"3.54.5"},"reference-count":63,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T00:00:00Z","timestamp":1649808000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008871","name":"Yunnan Provincial Science and Technology Department","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017720","name":"Kunming Science and Technology Bureau","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100017720","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:p>Pertussis, caused by the gram-negative bacterium <jats:italic>Bordetella pertussis<\/jats:italic>, is a highly contagious respiratory disease. Intranasal vaccination is an ideal strategy to prevent pertussis, as the nasal mucosa represents the first-line barrier to <jats:italic>B. pertussis<\/jats:italic> infection. The current intramuscular acellular pertussis (aP) vaccines elicit strong antibody and Th2-biased responses but not necessary cellular and mucosal immunity. Here, we formulated two cyclic dinucleotide (CDN)-adjuvanted aP subunit vaccines, a mammalian 2\u2019,3\u2019-cGAMP-adjuvanted aP vaccine and a bacterial-derived c-di-GMP-adjuvanted aP vaccine, and evaluated their immunogenicity in a mouse model. We found that the aP vaccine alone delivered intranasally (IN) induced moderate systemic and mucosal humoral immunity but weak cellular immunity, whereas the alum-adjuvanted aP vaccine administered intraperitoneally elicited higher Th2 and systemic humoral immune responses but weaker Th1 and Th17 and mucosal immune responses. In contrast, both CDN-adjuvanted aP vaccines administered <jats:italic>via<\/jats:italic> the IN route induced robust humoral and cellular immunity systemically and mucosally. Furthermore, the c-di-GMP-adjuvanted aP vaccine generated better antibody production and stronger Th1 and Th17 responses than the 2\u2032,3\u2032-cGAMP-adjuvanted aP vaccine. In addition, following <jats:italic>B. pertussis<\/jats:italic> challenge, the group of mice that received IN immunization with the c-di-GMP-adjuvanted aP vaccine showed better protection than all other groups of vaccinated mice, with decreased inflammatory cell infiltration in the lung and reduced bacterial burden in both the upper and lower respiratory tracts. In summary, the c-di-GMP-adjuvanted aP vaccine can elicit a multifaceted potent immune response resulting in robust bacterial clearance in the respiratory tract, which indicates that c-di-GMP can serve as a potential mucosal adjuvant for the pertussis vaccine.<\/jats:p>","DOI":"10.3389\/fimmu.2022.878832","type":"journal-article","created":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T07:17:49Z","timestamp":1649834269000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":17,"title":["Intranasal Immunization With a c-di-GMP-Adjuvanted Acellular Pertussis Vaccine Provides Superior Immunity Against Bordetella pertussis in a Mouse Model"],"prefix":"10.3389","volume":"13","author":[{"given":"Wenwen","family":"Jiang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhao","family":"Su","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lukui","family":"Cai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingyan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiangli","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qin","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingbo","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,4,13]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1586\/14760584.2015.1059759","article-title":"Changing From Whole-Cell to Acellular Pertussis Vaccines Would Trade Superior Tolerability for Inferior Protection","volume":"14","author":"Herzog","year":"2015","journal-title":"Expert Rev Vaccines"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2018.08.022","article-title":"Comparison of Adverse Events Following Immunisation With Acellular and Whole-Cell Pertussis Vaccines: A Systematic Review","volume":"36","author":"Patterson","year":"2018","journal-title":"Vaccine"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.Es.2019.24.7.1700832","article-title":"Pertactin-Deficient Bordetella Pertussis Isolates: Evidence of Increased Circulation in Europe, 1998 to 2015","volume":"24","author":"Barkoff","year":"2019","journal-title":"Euro Surveill"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.Es.2021.26.37.2001213","article-title":"Evolution of Bordetella Pertussis Over a 23-Year Period in France, 1996 to 2018","volume":"26","author":"Bouchez","year":"2021","journal-title":"Euro Surveill"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1200850","article-title":"Waning Protection After Fifth Dose of Acellular Pertussis Vaccine in Children","volume":"367","author":"Klein","year":"2012","journal-title":"N Engl J Med"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2012-3836","article-title":"Comparative Effectiveness of Acellular Versus Whole-Cell Pertussis Vaccines in Teenagers","volume":"131","author":"Klein","year":"2013","journal-title":"Pediatrics"},{"key":"B7","doi-asserted-by":"publisher","first-page":"146","DOI":"10.1186\/s12916-015-0382-8","article-title":"Asymptomatic Transmission and the Resurgence of Bordetella Pertussis","volume":"13","author":"Althouse","year":"2015","journal-title":"BMC Med"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1314688110","article-title":"Acellular Pertussis Vaccines Protect Against Disease But Fail to Prevent Infection and Transmission in a Nonhuman Primate Model","volume":"111","author":"Warfel","year":"2014","journal-title":"Proc Natl Acad Sci USA"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciz531","article-title":"Asymptomatic Infection and Transmission of Pertussis in Households: A Systematic Review","volume":"70","author":"Craig","year":"2020","journal-title":"Clin Infect Dis"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.65663","article-title":"Asymptomatic Bordetella Pertussis Infections in a Longitudinal Cohort of Young African Infants and Their Mothers","volume":"10","author":"Gill","year":"2021","journal-title":"Elife"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1097\/00054725-199911000-00009","article-title":"Th1\/Th2 Cells","volume":"5","author":"Romagnani","year":"1999","journal-title":"Inflamm Bowel Dis"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.03068","article-title":"The Role of Mucosal Immunity in Pertussis","volume":"9","author":"Solans","year":"2019","journal-title":"Front Immunol"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2012.117","article-title":"Bordetella Pertussis Infection Induces a Mucosal IL-17 Response and Long-Lived Th17 and Th1 Immune Memory Cells in Nonhuman Primates","volume":"6","author":"Warfel","year":"2013","journal-title":"Mucosal Immunol"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1038\/s41385-018-0080-x","article-title":"Sustained Protective Immunity Against Bordetella Pertussis Nasal Colonization by Intranasal Immunization With a Vaccine-Adjuvant Combination That Induces IL-17-Secreting T(RM) Cells","volume":"11","author":"Allen","year":"2018","journal-title":"Mucosal Immunol"},{"key":"B15","doi-asserted-by":"publisher","first-page":"40","DOI":"10.1038\/s41541-019-0136-2","article-title":"Intranasal Acellular Pertussis Vaccine Provides Mucosal Immunity and Protects Mice From Bordetella Pertussis","volume":"4","author":"Boehm","year":"2019","journal-title":"NPJ Vaccines"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1128\/iai.00346-21","article-title":"Mucosal Immunization With DTaP Confers Protection Against Bordetella Pertussis Infection and Cough in Sprague-Dawley Rats","volume":"89","author":"Hall","year":"2021","journal-title":"Infect Immun"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines9080917","article-title":"The Promise and Challenges of Cyclic Dinucleotides as Molecular Adjuvants for Vaccine Development","volume":"9","author":"Yan","year":"2021","journal-title":"Vaccines (Basel)"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2010.02.081","article-title":"The Potential of 3\u2019,5\u2019-Cyclic Diguanylic Acid (C-Di-GMP) as an Effective Vaccine Adjuvant","volume":"28","author":"Chen","year":"2010","journal-title":"Vaccine"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.01223","article-title":"Mucosal Administration of Cycle-Di-Nucleotide-Adjuvanted Virosomes Efficiently Induces Protection Against Influenza H5N1 in Mice","volume":"8","author":"Ebensen","year":"2017","journal-title":"Front Immunol"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2017.02.064","article-title":"Sublingual Targeting of STING With 3\u20193\u2019-cGAMP Promotes Systemic and Mucosal Immunity Against Anthrax Toxins","volume":"35","author":"Martin","year":"2017","journal-title":"Vaccine"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.02274","article-title":"Enhancing Immune Response and Heterosubtypic Protection Ability of Inactivated H7N9 Vaccine by Using STING Agonist as a Mucosal Adjuvant","volume":"10","author":"Luo","year":"2019","journal-title":"Front Immunol"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.08.014","article-title":"Cyclic Dinucleotides and the Innate Immune Response","volume":"154","author":"Danilchanka","year":"2013","journal-title":"Cell"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1038\/325279a0","article-title":"Regulation of Cellulose Synthesis in Acetobacter Xylinum by Cyclic Diguanylic Acid","volume":"325","author":"Ross","year":"1987","journal-title":"Nature"},{"key":"B24","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1128\/mmbr.00043-12","article-title":"Cyclic Di-GMP: The First 25 Years of a Universal Bacterial Second Messenger","volume":"77","author":"R\u00f6mling","year":"2013","journal-title":"Microbiol Mol Biol Rev"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.01.093","article-title":"3\u2019,5\u2019-Cyclic Diguanylic Acid (C-Di-GMP) Inhibits Basal and Growth Factor-Stimulated Human Colon Cancer Cell Proliferation","volume":"329","author":"Karaolis","year":"2005","journal-title":"Biochem Biophys Res Commun"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1126\/science.1232458","article-title":"Cyclic GMP-AMP Synthase is a Cytosolic DNA Sensor That Activates the Type I Interferon Pathway","volume":"339","author":"Sun","year":"2013","journal-title":"Science"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2013.05.022","article-title":"Cyclic GMP-AMP Containing Mixed Phosphodiester Linkages is an Endogenous High-Affinity Ligand for STING","volume":"51","author":"Zhang","year":"2013","journal-title":"Mol Cell"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2022.114934","article-title":"Second Messenger 2\u20193\u2019-Cyclic GMP-AMP (2\u20193\u2019-cGAMP):Synthesis, Transmission, and Degradation","volume":"198","author":"Su","year":"2022","journal-title":"Biochem Pharmacol"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2719-5","article-title":"STING Cyclic Dinucleotide Sensing Originated in Bacteria","volume":"586","author":"Morehouse","year":"2020","journal-title":"Nature"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.44.5.1899-1902.2006","article-title":"Development and Evaluation of a Loop-Mediated Isothermal Amplification Method for Rapid Diagnosis of Bordetella Pertussis Infection","volume":"44","author":"Kamachi","year":"2006","journal-title":"J Clin Microbiol"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2021.110112","article-title":"A Two-Adjuvant Multiantigen Candidate Vaccine Induces Superior Protective Immune Responses Against SARS-CoV-2 Challenge","volume":"37","author":"Jiang","year":"2021","journal-title":"Cell Rep"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2013.12.025","article-title":"Dynamic Profiles of Neutralizing Antibody Responses Elicited in Rhesus Monkeys Immunized With a Combined Tetravalent DTaP-Sabin IPV Candidate Vaccine","volume":"32","author":"Sun","year":"2014","journal-title":"Vaccine"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-63998-2","article-title":"Intranasal Immunization With Outer Membrane Vesicle Pertussis Vaccine Confers Broad Protection Through Mucosal IgA and Th17 Responses","volume":"10","author":"Raeven","year":"2020","journal-title":"Sci Rep"},{"key":"B34","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1016\/S1081-1206(10)62426-X","article-title":"T-Cell Subsets (Th1 Versus Th2)","volume":"85","author":"Romagnani","year":"2000","journal-title":"Ann Allergy Asthma Immunol"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp1137","article-title":"The Sanger FASTQ File Format for Sequences With Quality Scores, and the Solexa\/Illumina FASTQ Variants","volume":"38","author":"Cock","year":"2010","journal-title":"Nucleic Acids Res"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3317","article-title":"HISAT: A Fast Spliced Aligner With Low Memory Requirements","volume":"12","author":"Kim","year":"2015","journal-title":"Nat Methods"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated Estimation of Fold Change and Dispersion for RNA-Seq Data With Deseq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol"},{"key":"B38","doi-asserted-by":"publisher","first-page":"407","DOI":"10.1186\/s12879-021-06090-y","article-title":"Infant Rhesus Macaques as a non-Human Primate Model of Bordetella Pertussis Infection","volume":"21","author":"Jiang","year":"2021","journal-title":"BMC Infect Dis"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.12.045","article-title":"The Member of the Cyclic Di-Nucleotide Family Bis-(3\u2019, 5\u2019)-Cyclic Dimeric Inosine Monophosphate Exerts Potent Activity as Mucosal Adjuvant","volume":"28","author":"Libanova","year":"2010","journal-title":"Vaccine"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.21037\/atm-20-2537","article-title":"Post Hoc Analysis of Two Clinical Trials to Compare the Immunogenicity and Safety of Different Polio Immunization Schedules in Chinese Infants","volume":"9","author":"Zhao","year":"2021","journal-title":"Ann Transl Med"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.06670","article-title":"The Mucosal Adjuvant Cyclic Di-GMP Enhances Antigen Uptake and Selectively Activates Pinocytosis-Efficient Cells In Vivo","volume":"4","author":"Blaauboer","year":"2015","journal-title":"Elife"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.1661","article-title":"Hydrolysis of 2\u20193\u2019-cGAMP by ENPP1 and Design of Nonhydrolyzable Analogs","volume":"10","author":"Li","year":"2014","journal-title":"Nat Chem Biol"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2020.03.001","article-title":"Small Molecules Targeting the Innate Immune cGAS\u2212STING\u2212TBK1 Signaling Pathway","volume":"10","author":"Ding","year":"2020","journal-title":"Acta Pharm Sin B"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003264","article-title":"Relative Contribution of Th1 and Th17 Cells in Adaptive Immunity to Bordetella Pertussis: Towards the Rational Design of an Improved Acellular Pertussis Vaccine","volume":"9","author":"Ross","year":"2013","journal-title":"PloS Pathog"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2019.03.006","article-title":"Pertussis Vaccines and Protective Immunity","volume":"59","author":"Kapil","year":"2019","journal-title":"Curr Opin Immunol"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2017.08.009","article-title":"Addition of a TLR7 Agonist to an Acellular Pertussis Vaccine Enhances Th1 and Th17 Responses and Protective Immunity in a Mouse Model","volume":"35","author":"Misiak","year":"2017","journal-title":"Vaccine"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1038\/s41385-018-0073-9","article-title":"IL-17-Dependent SIgA-Mediated Protection Against Nasal Bordetella Pertussis Infection by Live Attenuated BPZE1 Vaccine","volume":"11","author":"Solans","year":"2018","journal-title":"Mucosal Immunol"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.20.2.167-170.1984","article-title":"Characterization of Antibody Inhibiting Adherence of Bordetella Pertussis to Human Respiratory Epithelial Cells","volume":"20","author":"Tuomanen","year":"1984","journal-title":"J Clin Microbiol"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1038\/s41385-021-00407-5","article-title":"IL-17 Mediates Protective Immunity Against Nasal Infection With Bordetella Pertussis by Mobilizing Neutrophils, Especially Siglec-F(+) Neutrophils","volume":"14","author":"Borkner","year":"2021","journal-title":"Mucosal Immunol"},{"key":"B50","doi-asserted-by":"publisher","first-page":"399","DOI":"10.1128\/iai.61.2.399-410.1993","article-title":"Cell-Mediated Immunity to Bordetella Pertussis: Role of Th1 Cells in Bacterial Clearance in a Murine Respiratory Infection Model","volume":"61","author":"Mills","year":"1993","journal-title":"Infect Immun"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1128\/iai.61.8.3190-3198.1993","article-title":"Effective Immunization Against Bordetella Pertussis Respiratory Infection in Mice is Dependent on Induction of Cell-Mediated Immunity","volume":"61","author":"Redhead","year":"1993","journal-title":"Infect Immun"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1086\/593682","article-title":"Bordetella Pertussis Respiratory Infection in Children is Associated With Preferential Activation of Type 1 T Helper Cells","volume":"175","author":"Ryan","year":"1997","journal-title":"J Infect Dis"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.170.3.1504","article-title":"Bordetella Pertussis Infection in 2-Month-Old Infants Promotes Type 1 T Cell Responses","volume":"170","author":"Mascart","year":"2003","journal-title":"J Immunol"},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.1084\/jem.186.11.1843","article-title":"Atypical Disease After Bordetella Pertussis Respiratory Infection of Mice With Targeted Disruptions of Interferon-Gamma Receptor or Immunoglobulin Mu Chain Genes","volume":"186","author":"Mahon","year":"1997","journal-title":"J Exp Med"},{"key":"B55","doi-asserted-by":"publisher","first-page":"899","DOI":"10.1016\/j.vaccine.2007.12.012","article-title":"Supplementation of Whole-Cell Pertussis Vaccines With Lipopolysaccharide Analogs: Modification of Vaccine-Induced Immune Responses","volume":"26","author":"Geurtsen","year":"2008","journal-title":"Vaccine"},{"key":"B56","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2016.08.059","article-title":"PLGA Nano\/Micro Particles Encapsulated With Pertussis Toxoid (PTd) Enhances Th1\/Th17 Immune Response in a Murine Model","volume":"513","author":"Li","year":"2016","journal-title":"Int J Pharm"},{"key":"B57","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines8040695","article-title":"Acellular Pertussis Vaccine Inhibits Bordetella Pertussis Clearance From the Nasal Mucosa of Mice","volume":"8","author":"Holubov\u00e1","year":"2020","journal-title":"Vaccines (Basel)"},{"key":"B58","doi-asserted-by":"publisher","first-page":"591","DOI":"10.1016\/j.jaci.2015.07.050","article-title":"Intrinsic Functional Defects of Type 2 Innate Lymphoid Cells Impair Innate Allergic Inflammation in Promyelocytic Leukemia Zinc Finger (PLZF)-Deficient Mice","volume":"137","author":"Verhoef","year":"2016","journal-title":"J Allergy Clin Immunol"},{"key":"B59","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.202003-0543OC","article-title":"Clinical Features of 85 Fatal Cases of COVID-19 From Wuhan. A Retrospective Observational Study","volume":"201","author":"Du","year":"2020","journal-title":"Am J Respir Crit Care Med"},{"key":"B60","doi-asserted-by":"publisher","DOI":"10.1016\/s0140-6736(20)30566-3","article-title":"Clinical Course and Risk Factors for Mortality of Adult Inpatients With COVID-19 in Wuhan, China: A Retrospective Cohort Study","volume":"395","author":"Zhou","year":"2020","journal-title":"Lancet"},{"key":"B61","doi-asserted-by":"publisher","DOI":"10.1128\/iai.00306-09","article-title":"Mouse Eosinophils Possess Potent Antibacterial Properties In Vivo","volume":"77","author":"Linch","year":"2009","journal-title":"Infect Immun"},{"key":"B62","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2020-0166OC","article-title":"IL-33-Mediated Eosinophilia Protects Against Acute Lung Injury","volume":"64","author":"Krishack","year":"2021","journal-title":"Am J Respir Cell Mol Biol"},{"key":"B63","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1038\/s41541-020-00270-8","article-title":"Suppression of Mucosal Th17 Memory Responses by Acellular Pertussis Vaccines Enhances Nasal Bordetella Pertussis Carriage","volume":"6","author":"Dubois","year":"2021","journal-title":"NPJ Vaccines"}],"container-title":["Frontiers in Immunology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fimmu.2022.878832\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T07:17:54Z","timestamp":1649834274000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fimmu.2022.878832\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4,13]]},"references-count":63,"alternative-id":["10.3389\/fimmu.2022.878832"],"URL":"https:\/\/doi.org\/10.3389\/fimmu.2022.878832","relation":{},"ISSN":["1664-3224"],"issn-type":[{"value":"1664-3224","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,4,13]]},"article-number":"878832"}}