{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T03:18:17Z","timestamp":1782962297486,"version":"3.54.5"},"reference-count":147,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,6,10]],"date-time":"2022-06-10T00:00:00Z","timestamp":1654819200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:p>Single-cell RNA sequencing (scRNAseq) technology is still relatively new in the field of gastric cancer immunology but gaining significant traction. This technology now provides unprecedented insights into the intratumoral and intertumoral heterogeneities at the immunological, cellular, and molecular levels. Within the last few years, a volume of publications reported the usefulness of scRNAseq technology in identifying thus far elusive immunological mechanisms that may promote and impede gastric cancer development. These studies analyzed datasets generated from primary human gastric cancer tissues, metastatic ascites fluid from gastric cancer patients, and laboratory-generated data from<jats:italic>in vitro<\/jats:italic>and<jats:italic>in vivo<\/jats:italic>models of gastric diseases. In this review, we overview the exciting findings from scRNAseq datasets that uncovered the role of critical immune cells, including T cells, B cells, myeloid cells, mast cells, ILC2s, and other inflammatory stromal cells, like fibroblasts and endothelial cells. In addition, we also provide a synopsis of the initial scRNAseq findings on the interesting epithelial cell responses to inflammation. In summary, these new studies have implicated roles for T and B cells and subsets like NKT cells in tumor development and progression. The current studies identified diverse subsets of macrophages and mast cells in the tumor microenvironment, however, additional studies to determine their roles in promoting cancer growth are needed. Some groups specifically focus on the less prevalent ILC2 cell type that may contribute to early cancer development. ScRNAseq analysis also reveals that stromal cells, e.g., fibroblasts and endothelial cells, regulate inflammation and promote metastasis, making them key targets for future investigations. While evaluating the outcomes, we also highlight the gaps in the current findings and provide an assessment of what this technology holds for gastric cancer research in the coming years. With scRNAseq technology expanding rapidly, we stress the need for periodic review of the findings and assess the available scRNAseq analytical tools to guide future work on immunological mechanisms of gastric carcinogenesis. \u2003<\/jats:p>","DOI":"10.3389\/fimmu.2022.902017","type":"journal-article","created":{"date-parts":[[2022,6,10]],"date-time":"2022-06-10T15:15:03Z","timestamp":1654874103000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":19,"title":["Discovering Immune-Mediated Mechanisms of Gastric Carcinogenesis Through Single-Cell RNA Sequencing"],"prefix":"10.3389","volume":"13","author":[{"given":"Stella G.","family":"Hoft","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle D.","family":"Pherson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard J.","family":"DiPaolo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,6,10]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"209\u201349","DOI":"10.3322\/caac.21660","article-title":"Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries","volume":"71","author":"Hyuna Sung","year":"2021","journal-title":"CA Cancer J Clin"},{"key":"B2","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1111\/apm.1965.64.1.31","article-title":"The Two Histological Main Types of Gastric Carcinoma: Diffuse and So-Called Intestinal-Type Carcinoma. an Attempt at a Histo-Clinical Classification","volume":"64","author":"Lauren","year":"1965","journal-title":"Acta Pathol Microbiol Scand"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3850","article-title":"Molecular Analysis of Gastric Cancer Identifies Subtypes Associated With Distinct Clinical Outcomes","volume":"21","author":"Cristescu","year":"2015","journal-title":"Nat Med"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1038\/nature13480","article-title":"Comprehensive Molecular Characterization of Gastric Adenocarcinoma","volume":"513","year":"2014","journal-title":"Nature"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.28999","article-title":"Global Burden of Gastric Cancer Attributable to Helicobacter Pylori","volume":"136","author":"Plummer","year":"2015","journal-title":"Int J Cancer"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpg.2014.10.005","article-title":"Chronic Gastritis - An Update","volume":"28","author":"Varbanova","year":"2014","journal-title":"Best Pract Res Clin Gastroenterol"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2001.21187","article-title":"H(+),K(+)-Atpase (Proton Pump) is the Target Autoantigen of Th1-Type Cytotoxic T Cells in Autoimmune Gastritis","volume":"120","author":"D'Elios","year":"2001","journal-title":"Gastroenterology"},{"key":"B8","doi-asserted-by":"crossref","DOI":"10.4049\/jimmunol.158.2.962","article-title":"T Helper 1 Effector Cells Specific for Helicobacter Pylori in the Gastric Antrum of Patients With Peptic Ulcer Disease","volume":"158","author":"D'Elios","year":"1997","journal-title":"J Immunol"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-12-3957","article-title":"Autoimmune Gastritis Mediated by CD4+ T Cells Promotes the Development of Gastric Cancer","volume":"73","author":"Nguyen","year":"2013","journal-title":"Cancer Res"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0901115","article-title":"Helicobacter Pylori-Induced Th17 Responses Modulate Th1 Cell Responses, Benefit Bacterial Growth, and Contribute to Pathology in Mice","volume":"184","author":"Shi","year":"2010","journal-title":"J Immunol"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.2.1022","article-title":"Helicobacter Pylori-Induced Mucosal Inflammation is Th1 Mediated and Exacerbated in IL-4, But Not IFN-Gamma, Gene-Deficient Mice","volume":"165","author":"Smythies","year":"2000","journal-title":"J Immunol"},{"key":"B12","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1186\/s13045-021-01105-2","article-title":"Applications of Single-Cell Sequencing in Cancer Research: Progress and Perspectives","volume":"14","author":"Lei","year":"2021","journal-title":"J Hematol Oncol"},{"key":"B13","doi-asserted-by":"publisher","first-page":"e20201574","DOI":"10.1084\/jem.20201574","article-title":"The Current Landscape of Single-Cell Transcriptomics for Cancer Immunotherapy","volume":"218","author":"Guruprasad","year":"2021","journal-title":"J Exp Med"},{"key":"B14","doi-asserted-by":"publisher","first-page":"453","DOI":"10.1053\/j.gastro.2020.04.073","article-title":"Use of Single-Cell -Omic Technologies to Study the Gastrointestinal Tract and Diseases, From Single Cell Identities to Patient Features","volume":"159","author":"Islam","year":"2020","journal-title":"Gastroenterology"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2020.03.008","article-title":"Advancing Cancer Research and Medicine With Single-Cell Genomics","volume":"37","author":"Lim","year":"2020","journal-title":"Cancer Cell"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2020.593085","article-title":"Progress and Clinical Application of Single-Cell Transcriptional Sequencing Technology in Cancer Research","volume":"10","author":"Liu","year":"2020","journal-title":"Front Oncol"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2020.00203","article-title":"Systems Biology of Gastric Cancer: Perspectives on the Omics-Based Diagnosis and Treatment","volume":"7","author":"Shi","year":"2020","journal-title":"Front Mol Biosci"},{"key":"B18","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1186\/s13046-021-01874-1","article-title":"Single-Cell RNA Sequencing in Cancer Research","volume":"40","author":"Zhang","year":"2021","journal-title":"J Exp Clin Cancer Res"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1315","article-title":"mRNA-Seq Whole-Transcriptome Analysis of a Single Cell","volume":"6","author":"Tang","year":"2009","journal-title":"Nat Methods"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.18632\/aging.102774","article-title":"Single-Cell RNA Sequencing of Immune Cells in Gastric Cancer Patients","volume":"12","author":"Fu","year":"2020","journal-title":"Aging (Albany NY)"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-21-0792","article-title":"Spatially Distinct Reprogramming of the Tumor Microenvironment Based On Tumor Invasion in Diffuse-Type Gastric Cancers","volume":"27","author":"Jeong","year":"2021","journal-title":"Clin Cancer Res"},{"key":"B22","doi-asserted-by":"publisher","first-page":"1969767","DOI":"10.1080\/2162402X.2021.1969767","article-title":"Single-Cell Profiling of Infiltrating B Cells and Tertiary Lymphoid Structures in the TME of Gastric Adenocarcinomas","volume":"10","author":"Jia","year":"2021","journal-title":"Oncoimmunology"},{"key":"B23","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1038\/s41698-022-00251-1","article-title":"Single-Cell Analysis of Gastric Pre-Cancerous and Cancer Lesions Reveals Cell Lineage Diversity and Intratumoral Heterogeneity","volume":"6","author":"Kim","year":"2022","journal-title":"NPJ Precis Oncol"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.7150\/thno.60540","article-title":"Single-Cell RNA Sequencing Reveals a Pro-Invasive Cancer-Associated Fibroblast Subgroup Associated With Poor Clinical Outcomes in Patients With Gastric Cancer","volume":"12","author":"Li","year":"2022","journal-title":"Theranostics"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1038\/s41388-021-01976-2","article-title":"Genomic and Transcriptomic Profiling of Hepatoid Adenocarcinoma of the Stomach","volume":"40","author":"Liu","year":"2021","journal-title":"Oncogene"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-19-3231","article-title":"Single-Cell Genomic Characterization Reveals the Cellular Reprogramming of the Gastric Tumor Microenvironment","volume":"26","author":"Sathe","year":"2020","journal-title":"Clin Cancer Res"},{"key":"B27","doi-asserted-by":"publisher","first-page":"1141","DOI":"10.1038\/s41598-020-80881-2","article-title":"Comprehensive Analysis of Metastatic Gastric Cancer Tumour Cells Using Single-Cell RNA-Seq","volume":"11","author":"Wang","year":"2021","journal-title":"Sci Rep"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2019-320368","article-title":"Dissecting Transcriptional Heterogeneity in Primary Gastric Adenocarcinoma by Single Cell RNA Sequencing","volume":"70","author":"Zhang","year":"2021","journal-title":"Gut"},{"key":"B29","doi-asserted-by":"publisher","first-page":"1934","DOI":"10.1016\/j.celrep.2019.04.052","article-title":"Dissecting the Single-Cell Transcriptome Network Underlying Gastric Premalignant Lesions and Early Gastric Cancer","volume":"27","author":"Zhang","year":"2019","journal-title":"Cell Rep"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.33873","article-title":"The Effects of TNF-\u03b1\/TNFR2 in Regulatory T Cells on the Microenvironment and Progression of Gastric Cancer","volume":"150","author":"Qu","year":"2022","journal-title":"Int J Cancer"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2021-324420","article-title":"Epigenetic Promoter Alterations in GI Tumour Immune-Editing and Resistance to Immune Checkpoint Inhibition","volume":"25","author":"Sundar","year":"2021","journal-title":"Gut"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.Cd-21-0219","article-title":"Determinants of Response and Intrinsic Resistance to PD-1 Blockade in Microsatellite Instability-High Gastric Cancer","volume":"11","author":"Kwon","year":"2021","journal-title":"Cancer Discovery"},{"key":"B33","doi-asserted-by":"publisher","first-page":"984","DOI":"10.1158\/2159-8290.Cd-21-0888","article-title":"Early Tumor-Immune Microenvironmental Remodeling and Response to First-Line Fluoropyrimidine and Platinum Chemotherapy in Advanced Gastric Cancer","volume":"12","author":"Kim","year":"2022","journal-title":"Cancer Discovery"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1002\/ctm2.730","article-title":"Revealing the Transcriptional Heterogeneity of Organ-Specific Metastasis in Human Gastric Cancer Using Single-Cell RNA Sequencing","volume":"12","author":"Jiang","year":"2022","journal-title":"Clin Transl Med"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.Cd-21-0683","article-title":"Single-Cell Atlas of Lineage States, Tumor Microenvironment, and Subtype-Specific Expression Programs in Gastric Cancer","volume":"12","author":"Kumar","year":"2022","journal-title":"Cancer Discovery"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2020.00940","article-title":"A Multi-Gene Model Effectively Predicts the Overall Prognosis of Stomach Adenocarcinomas With Large Genetic Heterogeneity Using Somatic Mutation Features","volume":"11","author":"Liu","year":"2020","journal-title":"Front Genet"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.7150\/jca.49707","article-title":"Cancer-Associated Fibroblasts in Gastric Cancer Affect Malignant Progression via the CXCL12-CXCR4 Axis","volume":"12","author":"Qin","year":"2021","journal-title":"J Cancer"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.729485","article-title":"Prognostic Autophagy-Related Model Revealed by Integrating Single-Cell RNA Sequencing Data and Bulk Gene Profiles in Gastric Cancer","volume":"9","author":"Tong","year":"2021","journal-title":"Front Cell Dev Biol"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.728169","article-title":"A Dynamic Transcriptome Map of Different Tissue Microenvironment Cells Identified During Gastric Cancer Development Using Single-Cell RNA Sequencing","volume":"12","author":"Yin","year":"2021","journal-title":"Front Immunol"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2020.597967","article-title":"Susceptibility Factors of Stomach for SARS-CoV-2 and Treatment Implication of Mucosal Protective Agent in COVID-19","volume":"7","author":"Zhang","year":"2020","journal-title":"Front Med (Lausanne)"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.720649","article-title":"Identification and Validation of a Malignant Cell Subset Marker-Based Polygenic Risk Score in Stomach Adenocarcinoma Through Integrated Analysis of Bulk and Single-Cell RNA Sequencing Data","volume":"9","author":"Zou","year":"2021","journal-title":"Front Cell Dev Biol"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13205175","article-title":"Co-Expression With Membrane CMTM6\/4 on Tumor Epithelium Enhances the Prediction Value of PD-L1 on Anti-PD-1\/L1 Therapeutic Efficacy in Gastric Adenocarcinoma","volume":"13","author":"Wang","year":"2021","journal-title":"Cancers (Basel)"},{"key":"B43","doi-asserted-by":"publisher","first-page":"1609955","DOI":"10.3389\/pore.2021.1609955","article-title":"Identifying Diagnostic and Prognostic Biomarkers and Candidate Therapeutic Drugs of Gastric Cancer Based on Transcriptomics and Single-Cell Sequencing","volume":"27","author":"Zhao","year":"2021","journal-title":"Pathol Oncol Res"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-020-1125-8","article-title":"Single-Cell Dissection of Intratumoral Heterogeneity and Lineage Diversity in Metastatic Gastric Adenocarcinoma","volume":"27","author":"Wang","year":"2021","journal-title":"Nat Med"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1038\/s12276-020-00538-y","article-title":"Tumor-Promoting Macrophages Prevail in Malignant Ascites of Advanced Gastric Cancer","volume":"52","author":"Eum","year":"2020","journal-title":"Exp Mol Med"},{"key":"B46","doi-asserted-by":"publisher","first-page":"2116","DOI":"10.1053\/j.gastro.2020.08.027","article-title":"Single-Cell Transcriptional Analyses Identify Lineage-Specific Epithelial Responses to Inflammation and Metaplastic Development in the Gastric Corpus","volume":"159","author":"Bockerstett","year":"2020","journal-title":"Gastroenterology"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2019-318930","article-title":"Single-Cell Transcriptional Analyses of Spasmolytic Polypeptide-Expressing Metaplasia Arising From Acute Drug Injury and Chronic Inflammation in the Stomach","volume":"69","author":"Bockerstett","year":"2020","journal-title":"Gut"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcmgh.2020.04.014","article-title":"Interleukin 27 Protects From Gastric Atrophy and Metaplasia During Chronic Autoimmune Gastritis","volume":"10","author":"Bockerstett","year":"2020","journal-title":"Cell Mol Gastroenterol Hepatol"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcmgh.2021.09.012","article-title":"IL-13 Acts Directly on Gastric Epithelial Cells to Promote Metaplasia Development During Chronic Gastritis","volume":"13","author":"Noto","year":"2021","journal-title":"Cell Mol Gastroenterol Hepatol"},{"key":"B50","doi-asserted-by":"publisher","first-page":"637","DOI":"10.1053\/j.gastro.2021.04.075","article-title":"Glucocorticoids and Androgens Protect From Gastric Metaplasia by Suppressing Group 2 Innate Lymphoid Cell Activation","volume":"161","author":"Busada","year":"2021","journal-title":"Gastroenterology"},{"key":"B51","doi-asserted-by":"publisher","first-page":"2077","DOI":"10.1053\/j.gastro.2020.08.051","article-title":"Group 2 Innate Lymphoid Cells Coordinate Damage Response in the Stomach","volume":"159","author":"Meyer","year":"2020","journal-title":"Gastroenterology"},{"key":"B52","doi-asserted-by":"publisher","first-page":"4536","DOI":"10.1038\/s41598-019-40809-x","article-title":"Single-Cell Transcriptome Analysis Identifies Distinct Cell Types and Niche Signaling in a Primary Gastric Organoid Model","volume":"9","author":"Chen","year":"2019","journal-title":"Sci Rep"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1002\/path.5675","article-title":"Modelling Hereditary Diffuse Gastric Cancer Initiation Using Transgenic Mouse-Derived Gastric Organoids and Single-Cell Sequencing","volume":"254","author":"Dixon","year":"2021","journal-title":"J Pathol"},{"key":"B54","doi-asserted-by":"publisher","first-page":"e001358","DOI":"10.1136\/jitc-2020-001358","article-title":"Deep Immunophenotyping at the Single-Cell Level Identifies a Combination of Anti-IL-17 and Checkpoint Blockade as an Effective Treatment in a Preclinical Model of Data-Guided Personalized Immunotherapy","volume":"8","author":"Nagaoka","year":"2020","journal-title":"J Immunother Cancer"},{"key":"B55","doi-asserted-by":"publisher","first-page":"lqaa016","DOI":"10.1093\/nargab\/lqaa016","article-title":"Joint Single Cell DNA-Seq and RNA-Seq of Gastric Cancer Cell Lines Reveals Rules of In Vitro Evolution","volume":"2","author":"Andor","year":"2020","journal-title":"NAR Genom Bioinform"},{"key":"B56","doi-asserted-by":"publisher","first-page":"342","DOI":"10.1016\/j.stem.2019.07.008","article-title":"Defining the Identity and Dynamics of Adult Gastric Isthmus Stem Cells","volume":"25","author":"Han","year":"2019","journal-title":"Cell Stem Cell"},{"key":"B57","doi-asserted-by":"publisher","first-page":"108819","DOI":"10.1016\/j.celrep.2021.108819","article-title":"Human Gastrointestinal Epithelia of the Esophagus, Stomach, and Duodenum Resolved at Single-Cell Resolution","volume":"34","author":"Busslinger","year":"2021","journal-title":"Cell Rep"},{"key":"B58","doi-asserted-by":"publisher","DOI":"10.1242\/dev.188839","article-title":"Signalling Codes for the Maintenance and Lineage Commitment of Embryonic Gastric Epithelial Progenitors","volume":"147","author":"Sayols","year":"2020","journal-title":"Development"},{"key":"B59","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-018-0105-4","article-title":"Tracing the Temporal-Spatial Transcriptome Landscapes of the Human Fetal Digestive Tract Using Single-Cell RNA-Sequencing","volume":"20","author":"Gao","year":"2018","journal-title":"Nat Cell Biol"},{"key":"B60","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.11.019","article-title":"Autophagy-Mediated Tumor Promotion","volume":"155","author":"Guo","year":"2013","journal-title":"Cell"},{"key":"B61","doi-asserted-by":"publisher","first-page":"1727","DOI":"10.1053\/j.gastro.2014.02.007","article-title":"Macrophages Promote Progression of Spasmolytic Polypeptide-Expressing Metaplasia After Acute Loss of Parietal Cells","volume":"146","author":"Petersen","year":"2014","journal-title":"Gastroenterology"},{"key":"B62","doi-asserted-by":"publisher","first-page":"678","DOI":"10.1016\/j.jcmgh.2017.12.012","article-title":"Interleukin-17a Promotes Parietal Cell Atrophy by Inducing Apoptosis","volume":"5","author":"Bockerstett","year":"2018","journal-title":"Cell Mol Gastroenterol Hepatol"},{"key":"B63","doi-asserted-by":"publisher","DOI":"10.1002\/path.5214","article-title":"Interferon-\u03b3 Directly Induces Gastric Epithelial Cell Death and is Required for Progression to Metaplasia","volume":"247","author":"Osaki","year":"2019","journal-title":"J Pathol"},{"key":"B64","doi-asserted-by":"publisher","DOI":"10.4161\/onci.25911","article-title":"A New Mouse Model of Inflammation and Gastric Cancer","volume":"2","author":"Nguyen","year":"2013","journal-title":"Oncoimmunology"},{"key":"B65","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s40620-015-0245-6","article-title":"Role of Complement in IgA Nephropathy","volume":"29","author":"Daha","year":"2016","journal-title":"J Nephrol"},{"key":"B66","doi-asserted-by":"publisher","first-page":"1081","DOI":"10.1016\/j.cell.2020.02.015","article-title":"Complement Signals Determine Opposite Effects of B Cells in Chemotherapy-Induced Immunity","volume":"180","author":"Lu","year":"2020","journal-title":"Cell"},{"key":"B67","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2004.08.061","article-title":"EBV-Positive Gastric Adenocarcinomas: A Distinct Clinicopathologic Entity With a Low Frequency of Lymph Node Involvement","volume":"22","author":"van Beek","year":"2004","journal-title":"J Clin Oncol"},{"key":"B68","doi-asserted-by":"publisher","DOI":"10.1038\/nri3073","article-title":"Protective and Pathogenic Functions of Macrophage Subsets","volume":"11","author":"Murray","year":"2011","journal-title":"Nat Rev Immunol"},{"key":"B69","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0105015","article-title":"Phenotypic and Functional Profiling of Human Proinflammatory Type-1 and Anti-Inflammatory Type-2 Macrophages in Response to Microbial Antigens and IFN-Gamma- and CD40L-Mediated Costimulation","volume":"79","author":"Verreck","year":"2006","journal-title":"J Leukoc Biol"},{"key":"B70","doi-asserted-by":"publisher","first-page":"1293","DOI":"10.1016\/j.cell.2018.05.060","article-title":"Single-Cell Map of Diverse Immune Phenotypes in the Breast Tumor Microenvironment","volume":"174","author":"Azizi","year":"2018","journal-title":"Cell"},{"key":"B71","doi-asserted-by":"publisher","first-page":"442","DOI":"10.1016\/j.cell.2020.03.048","article-title":"Single-Cell Analyses Inform Mechanisms of Myeloid-Targeted Therapies in Colon Cancer","volume":"181","author":"Zhang","year":"2020","journal-title":"Cell"},{"key":"B72","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2008.08.001","article-title":"Osteopontin: Role in Immune Regulation and Stress Responses","volume":"19","author":"Wang","year":"2008","journal-title":"Cytokine Growth Factor Rev"},{"key":"B73","doi-asserted-by":"publisher","first-page":"100","DOI":"10.1186\/s13045-018-0644-y","article-title":"Targeting the IDO1 Pathway in Cancer: From Bench to Bedside","volume":"11","author":"Liu","year":"2018","journal-title":"J Hematol Oncol"},{"key":"B74","doi-asserted-by":"publisher","first-page":"1","DOI":"10.4251\/wjgo.v13.i1.1","article-title":"Myeloid-Derived Suppressor Cells in Gastrointestinal Cancers: A Systemic Review","volume":"13","author":"Farshidpour","year":"2021","journal-title":"World J Gastrointest Oncol"},{"key":"B75","doi-asserted-by":"publisher","first-page":"dev188839","DOI":"10.1126\/sciimmunol.aay6017","article-title":"Defining the Emergence of Myeloid-Derived Suppressor Cells in Breast Cancer Using Single-Cell Transcriptomics","volume":"147","author":"Alshetaiwi","year":"2020","journal-title":"Sci Immunol"},{"key":"B76","doi-asserted-by":"publisher","first-page":"3715","DOI":"10.1038\/s41467-020-17492-y","article-title":"Single-Cell Transcriptomic Analysis in a Mouse Model Deciphers Cell Transition States in the Multistep Development of Esophageal Cancer","volume":"11","author":"Yao","year":"2020","journal-title":"Nat Commun"},{"key":"B77","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-0915-y","article-title":"Neutrophils Escort Circulating Tumour Cells to Enable Cell Cycle Progression","volume":"566","author":"Szczerba","year":"2019","journal-title":"Nature"},{"key":"B78","doi-asserted-by":"publisher","DOI":"10.3390\/biom11081232","article-title":"Pathophysiological Roles of Histamine Receptors in Cancer Progression: Implications and Perspectives as Potential Molecular Targets","volume":"11","author":"Nguyen","year":"2021","journal-title":"Biomolecules"},{"key":"B79","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2016-312779","article-title":"A Signalling Cascade of IL-33 to IL-13 Regulates Metaplasia in the Mouse Stomach","volume":"67","author":"Petersen","year":"2018","journal-title":"Gut"},{"key":"B80","doi-asserted-by":"publisher","DOI":"10.1172\/JCI123233","article-title":"Endogenous Glucocorticoids Prevent Gastric Metaplasia by Suppressing Spontaneous Inflammation","volume":"129","author":"Busada","year":"2019","journal-title":"J Clin Invest"},{"key":"B81","doi-asserted-by":"publisher","DOI":"10.1084\/jem.184.3.1101","article-title":"A Highly Efficacious Lymphocyte Chemoattractant, Stromal Cell-Derived Factor 1 (SDF-1)","volume":"184","author":"Bleul","year":"1996","journal-title":"J Exp Med"},{"key":"B82","doi-asserted-by":"publisher","DOI":"10.1038\/nature10694","article-title":"Interactions Between Cancer Stem Cells and Their Niche Govern Metastatic Colonization","volume":"481","author":"Malanchi","year":"2012","journal-title":"Nature"},{"key":"B83","doi-asserted-by":"publisher","DOI":"10.3390\/biology9060126","article-title":"STAT3 Pathway in Gastric Cancer: Signaling, Therapeutic Targeting and Future Prospects","volume":"9","author":"Ashrafizadeh","year":"2020","journal-title":"Biol (Basel)"},{"key":"B84","doi-asserted-by":"publisher","DOI":"10.21873\/anticanres.13879","article-title":"The Clinicopathological Significance of the CXCR2 Ligands, CXCL1, CXCL2, CXCL3, CXCL5, CXCL6, CXCL7, and CXCL8 in Gastric Cancer","volume":"39","author":"Yamamoto","year":"2019","journal-title":"Anticancer Res"},{"key":"B85","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2019.00317","article-title":"Single-Cell RNA-Seq Technologies and Related Computational Data Analysis","volume":"10","author":"Chen","year":"2019","journal-title":"Front Genet"},{"key":"B86","doi-asserted-by":"publisher","first-page":"14049","DOI":"10.1038\/ncomms14049","article-title":"Massively Parallel Digital Transcriptional Profiling of Single Cells","volume":"8","author":"Zheng","year":"2017","journal-title":"Nat Commun"},{"key":"B87","doi-asserted-by":"publisher","DOI":"10.1101\/gr.092759.109","article-title":"Circos: An Information Aesthetic for Comparative Genomics","volume":"19","author":"Krzywinski","year":"2009","journal-title":"Genome Res"},{"key":"B88","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1186\/s13059-016-0938-8","article-title":"CEL-Seq2: Sensitive Highly-Multiplexed Single-Cell RNA-Seq","volume":"17","author":"Hashimshony","year":"2016","journal-title":"Genome Biol"},{"key":"B89","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2639","article-title":"Smart-Seq2 for Sensitive Full-Length Transcriptome Profiling in Single Cells","volume":"10","author":"Picelli","year":"2013","journal-title":"Nat Methods"},{"key":"B90","doi-asserted-by":"publisher","first-page":"36014","DOI":"10.1038\/srep36014","article-title":"Vertical Flow Array Chips Reliably Identify Cell Types From Single-Cell mRNA Sequencing Experiments","volume":"6","author":"Shirai","year":"2016","journal-title":"Sci Rep"},{"key":"B91","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1007\/978-981-13-6037-4_5","article-title":"Quantitation of mRNA Transcripts and Proteins Using the BD Rhapsody\u2122 Single-Cell Analysis System","volume":"1129","author":"Shum","year":"2019","journal-title":"Adv Exp Med Biol"},{"key":"B92","doi-asserted-by":"publisher","DOI":"10.2174\/1389202921999200625220812","article-title":"The Comparison of Two Single-Cell Sequencing Platforms: BD Rhapsody and 10x Genomics Chromium","volume":"21","author":"Gao","year":"2020","journal-title":"Curr Genomics"},{"key":"B93","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","article-title":"STAR: Ultrafast Universal RNA-Seq Aligner","volume":"29","author":"Dobin","year":"2013","journal-title":"Bioinformatics"},{"key":"B94","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu638","article-title":"HTSeq\u2013a Python Framework to Work With High-Throughput Sequencing Data","volume":"31","author":"Anders","year":"2015","journal-title":"Bioinformatics"},{"key":"B95","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1186\/s13059-016-0947-7","article-title":"Pooling Across Cells to Normalize Single-Cell RNA Sequencing Data With Many Zero Counts","volume":"17","author":"Lun","year":"2016","journal-title":"Genome Biol"},{"key":"B96","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btw777","article-title":"Scater: Pre-Processing, Quality Control, Normalization and Visualization of Single-Cell RNA-Seq Data in R","volume":"33","author":"McCarthy","year":"2017","journal-title":"Bioinformatics"},{"key":"B97","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.4096","article-title":"Integrating Single-Cell Transcriptomic Data Across Different Conditions, Technologies, and Species","volume":"36","author":"Butler","year":"2018","journal-title":"Nat Biotechnol"},{"key":"B98","doi-asserted-by":"publisher","first-page":"3573","DOI":"10.1016\/j.cell.2021.04.048","article-title":"Integrated Analysis of Multimodal Single-Cell Data","volume":"184","author":"Hao","year":"2021","journal-title":"Cell"},{"key":"B99","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1186\/s13059-017-1382-0","article-title":"SCANPY: Large-Scale Single-Cell Gene Expression Data Analysis","volume":"19","author":"Wolf","year":"2018","journal-title":"Genome Biol"},{"key":"B100","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-018-0276-y","article-title":"Reference-Based Analysis of Lung Single-Cell Sequencing Reveals a Transitional Profibrotic Macrophage","volume":"20","author":"Aran","year":"2019","journal-title":"Nat Immunol"},{"key":"B101","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btm563","article-title":"Defining Clusters From a Hierarchical Cluster Tree: The Dynamic Tree Cut Package for R","volume":"24","author":"Langfelder","year":"2008","journal-title":"Bioinformatics"},{"key":"B102","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2157-4","article-title":"Construction of a Human Cell Landscape at Single-Cell Level","volume":"581","author":"Han","year":"2020","journal-title":"Nature"},{"key":"B103","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3337","article-title":"Robust Enumeration of Cell Subsets From Tissue Expression Profiles","volume":"12","author":"Newman","year":"2015","journal-title":"Nat Methods"},{"key":"B104","doi-asserted-by":"publisher","first-page":"128","DOI":"10.1186\/1471-2105-14-128","article-title":"Enrichr: Interactive and Collaborative HTML5 Gene List Enrichment Analysis Tool","volume":"14","author":"Chen","year":"2013","journal-title":"BMC Bioinf"},{"key":"B105","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw377","article-title":"Enrichr: A Comprehensive Gene Set Enrichment Analysis Web Server 2016 Update","volume":"44","author":"Kuleshov","year":"2016","journal-title":"Nucleic Acids Res"},{"key":"B106","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0506580102","article-title":"Gene Set Enrichment Analysis: A Knowledge-Based Approach for Interpreting Genome-Wide Expression Profiles","volume":"102","author":"Subramanian","year":"2005","journal-title":"Proc Natl Acad Sci U.S.A."},{"key":"B107","doi-asserted-by":"publisher","first-page":"1523","DOI":"10.1038\/s41467-019-09234-6","article-title":"Metascape Provides a Biologist-Oriented Resource for the Analysis of Systems-Level Datasets","volume":"10","author":"Zhou","year":"2019","journal-title":"Nat Commun"},{"key":"B108","doi-asserted-by":"publisher","DOI":"10.1089\/omi.2011.0118","article-title":"Clusterprofiler: An R Package for Comparing Biological Themes Among Gene Clusters","volume":"16","author":"Yu","year":"2012","journal-title":"Omics"},{"key":"B109","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1186\/1471-2105-14-7","article-title":"GSVA: Gene Set Variation Analysis for Microarray and RNA-Seq Data","volume":"14","author":"H\u00e4nzelmann","year":"2013","journal-title":"BMC Bioinf"},{"key":"B110","doi-asserted-by":"publisher","first-page":"W130","DOI":"10.1093\/nar\/gkx356","article-title":"WebGestalt 2017: A More Comprehensive, Powerful, Flexible and Interactive Gene Set Enrichment Analysis Toolkit","volume":"45","author":"Wang","year":"2017","journal-title":"Nucleic Acids Res"},{"key":"B111","doi-asserted-by":"publisher","DOI":"10.1016\/j.cels.2015.12.004","article-title":"The Molecular Signatures Database (MSigDB) Hallmark Gene Set Collection","volume":"1","author":"Liberzon","year":"2015","journal-title":"Cell Syst"},{"key":"B112","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt1102","article-title":"The Reactome Pathway Knowledgebase","volume":"42","author":"Croft","year":"2014","journal-title":"Nucleic Acids Res"},{"key":"B113","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4463","article-title":"SCENIC: Single-Cell Regulatory Network Inference and Clustering","volume":"14","author":"Aibar","year":"2017","journal-title":"Nat Methods"},{"key":"B114","doi-asserted-by":"publisher","first-page":"7866","DOI":"10.1038\/ncomms8866","article-title":"A Draft Network of Ligand-Receptor-Mediated Multicellular Signalling in Human","volume":"6","author":"Ramilowski","year":"2015","journal-title":"Nat Commun"},{"key":"B115","doi-asserted-by":"publisher","first-page":"D607","DOI":"10.1093\/nar\/gky1131","article-title":"STRING V11: Protein-Protein Association Networks With Increased Coverage, Supporting Functional Discovery in Genome-Wide Experimental Datasets","volume":"47","author":"Szklarczyk","year":"2019","journal-title":"Nucleic Acids Res"},{"key":"B116","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0698-6","article-title":"Single-Cell Reconstruction of the Early Maternal-Fetal Interface in Humans","volume":"563","author":"Vento-Tormo","year":"2018","journal-title":"Nature"},{"key":"B117","doi-asserted-by":"publisher","DOI":"10.1038\/s41596-020-0292-x","article-title":"CellPhoneDB: Inferring Cell-Cell Communication From Combined Expression of Multi-Subunit Ligand-Receptor Complexes","volume":"15","author":"Efremova","year":"2020","journal-title":"Nat Protoc"},{"key":"B118","doi-asserted-by":"publisher","first-page":"1088","DOI":"10.1038\/s41467-021-21246-9","article-title":"Inference and Analysis of Cell-Cell Communication Using CellChat","volume":"12","author":"Jin","year":"2021","journal-title":"Nat Commun"},{"key":"B119","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2859","article-title":"The Dynamics and Regulators of Cell Fate Decisions are Revealed by Pseudotemporal Ordering of Single Cells","volume":"32","author":"Trapnell","year":"2014","journal-title":"Nat Biotechnol"},{"key":"B120","doi-asserted-by":"publisher","first-page":"477","DOI":"10.1186\/s12864-018-4772-0","article-title":"Slingshot: Cell Lineage and Pseudotime Inference for Single-Cell Transcriptomics","volume":"19","author":"Street","year":"2018","journal-title":"BMC Genomics"},{"key":"B121","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201646347","article-title":"Computational Methods for Trajectory Inference From Single-Cell Transcriptomics","volume":"46","author":"Cannoodt","year":"2016","journal-title":"Eur J Immunol"},{"key":"B122","doi-asserted-by":"publisher","first-page":"46","DOI":"10.1093\/nar\/gkab1132","article-title":"MarkovHC: Markov Hierarchical Clustering for the Topological Structure of High-Dimensional Single-Cell Omics Data With Transition Pathway and Critical Point Detection","volume":"50","author":"Wang","year":"2022","journal-title":"Nucleic Acids Res"},{"key":"B123","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0414-6","article-title":"RNA Velocity of Single Cells","volume":"560","author":"La Manno","year":"2018","journal-title":"Nature"},{"key":"B124","doi-asserted-by":"publisher","DOI":"10.1126\/science.1254257","article-title":"Single-Cell RNA-Seq Highlights Intratumoral Heterogeneity in Primary Glioblastoma","volume":"344","author":"Patel","year":"2014","journal-title":"Science"},{"key":"B125","doi-asserted-by":"publisher","first-page":"R41","DOI":"10.1186\/gb-2011-12-4-r41","article-title":"GISTIC2.0 Facilitates Sensitive and Confident Localization of the Targets of Focal Somatic Copy-Number Alteration in Human Cancers","volume":"12","author":"Mermel","year":"2011","journal-title":"Genome Biol"},{"key":"B126","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2514","article-title":"Sensitive Detection of Somatic Point Mutations in Impure and Heterogeneous Cancer Samples","volume":"31","author":"Cibulskis","year":"2013","journal-title":"Nat Biotechnol"},{"key":"B127","doi-asserted-by":"publisher","first-page":"599","DOI":"10.1038\/s41587-020-00795-2","article-title":"Delineating Copy Number and Clonal Substructure in Human Tumors From Single-Cell Transcriptomes","volume":"39","author":"Gao","year":"2021","journal-title":"Nat Biotechnol"},{"key":"B128","doi-asserted-by":"publisher","DOI":"10.1007\/s10549-009-0674-9","article-title":"An Online Survival Analysis Tool to Rapidly Assess the Effect of 22,277 Genes on Breast Cancer Prognosis Using Microarray Data of 1,809 Patients","volume":"123","author":"Gy\u00f6rffy","year":"2010","journal-title":"Breast Cancer Res Treat"},{"key":"B129","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1038\/msb.2008.27","article-title":"Network-Based Global Inference of Human Disease Genes","volume":"4","author":"Wu","year":"2008","journal-title":"Mol Syst Biol"},{"key":"B130","doi-asserted-by":"publisher","first-page":"D900","DOI":"10.1093\/nar\/gky939","article-title":"CancerSEA: A Cancer Single-Cell State Atlas","volume":"47","author":"Yuan","year":"2019","journal-title":"Nucleic Acids Res"},{"key":"B131","doi-asserted-by":"publisher","first-page":"W556","DOI":"10.1093\/nar\/gkz430","article-title":"GEPIA2: An Enhanced Web Server for Large-Scale Expression Profiling and Interactive Analysis","volume":"47","author":"Tang","year":"2019","journal-title":"Nucleic Acids Res"},{"key":"B132","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-020-0546-8","article-title":"Visualizing and Interpreting Cancer Genomics Data via the Xena Platform","volume":"38","author":"Goldman","year":"2020","journal-title":"Nat Biotechnol"},{"key":"B133","doi-asserted-by":"publisher","first-page":"400","DOI":"10.1016\/j.cell.2018.02.052","article-title":"An Integrated TCGA Pan-Cancer Clinical Data Resource to Drive High-Quality Survival Outcome Analytics","volume":"173","author":"Liu","year":"2018","journal-title":"Cell"},{"key":"B134","doi-asserted-by":"publisher","DOI":"10.1093\/database\/baw160","article-title":"FirebrowseR: An R Client to the Broad Institute's Firehose Pipeline","volume":"2017","author":"Deng","year":"2017","journal-title":"Database (Oxford)"},{"key":"B135","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1209279","article-title":"Systematic Search for Gastric Cancer-Specific Genes Based on SAGE Data: Melanoma Inhibitory Activity and Matrix Metalloproteinase-10 are Novel Prognostic Factors in Patients With Gastric Cancer","volume":"25","author":"Aung","year":"2006","journal-title":"Oncogene"},{"key":"B136","doi-asserted-by":"publisher","DOI":"10.1038\/s41422-020-0353-2","article-title":"Reconstruction of Cell Spatial Organization From Single-Cell RNA Sequencing Data Based on Ligand-Receptor Mediated Self-Assembly","volume":"30","author":"Ren","year":"2020","journal-title":"Cell Res"},{"key":"B137","doi-asserted-by":"publisher","DOI":"10.1126\/science.aad0501","article-title":"Dissecting the Multicellular Ecosystem of Metastatic Melanoma by Single-Cell RNA-Seq","volume":"352","author":"Tirosh","year":"2016","journal-title":"Science"},{"key":"B138","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2203","article-title":"Absolute Quantification of Somatic DNA Alterations in Human Cancer","volume":"30","author":"Carter","year":"2012","journal-title":"Nat Biotechnol"},{"key":"B139","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.Can-17-0307","article-title":"TIMER: A Web Server for Comprehensive Analysis of Tumor-Infiltrating Immune Cells","volume":"77","author":"Li","year":"2017","journal-title":"Cancer Res"},{"key":"B140","doi-asserted-by":"publisher","first-page":"D1083","DOI":"10.1093\/nar\/gkaa968","article-title":"CellMiner Cross-Database (CellMinerCDB) Version 1.2: Exploration of Patient-Derived Cancer Cell Line Pharmacogenomics","volume":"49","author":"Luna","year":"2021","journal-title":"Nucleic Acids Res"},{"key":"B141","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1038\/s41592-020-01033-y","article-title":"Method of the Year: Spatially Resolved Transcriptomics","volume":"18","author":"Marx","year":"2021","journal-title":"Nat Methods"},{"key":"B142","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/bbaa145","article-title":"Spatial Molecular Profiling: Platforms, Applications and Analysis Tools","volume":"22","author":"Zhang","year":"2021","journal-title":"Brief Bioinform"},{"key":"B143","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23063042","article-title":"Single-Cell RNA Sequencing With Spatial Transcriptomics of Cancer Tissues","volume":"23","author":"Ahmed","year":"2022","journal-title":"Int J Mol Sci"},{"key":"B144","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4380","article-title":"Simultaneous Epitope and Transcriptome Measurement in Single Cells","volume":"14","author":"Stoeckius","year":"2017","journal-title":"Nat Methods"},{"key":"B145","doi-asserted-by":"publisher","first-page":"297","DOI":"10.1038\/nmeth.4177","article-title":"Pooled CRISPR Screening With Single-Cell Transcriptome Readout","volume":"14","author":"Datlinger","year":"2017","journal-title":"Nat Methods"},{"key":"B146","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4604","article-title":"On the Design of CRISPR-Based Single-Cell Molecular Screens","volume":"15","author":"Hill","year":"2018","journal-title":"Nat Methods"},{"key":"B147","doi-asserted-by":"publisher","first-page":"100043","DOI":"10.1016\/j.bioflm.2021.100043","article-title":"Do Results Obtained With RNA-Sequencing Require Independent Verification","volume":"3","author":"Coenye","year":"2021","journal-title":"Biofilm"}],"container-title":["Frontiers in Immunology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fimmu.2022.902017\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,7]],"date-time":"2023-02-07T22:01:29Z","timestamp":1675807289000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fimmu.2022.902017\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,10]]},"references-count":147,"alternative-id":["10.3389\/fimmu.2022.902017"],"URL":"https:\/\/doi.org\/10.3389\/fimmu.2022.902017","relation":{},"ISSN":["1664-3224"],"issn-type":[{"value":"1664-3224","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,10]]},"article-number":"902017"}}