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Immunol."],"abstract":"<jats:p>Classical swine fever virus (CSFV) is a major animal pathogen threatening the global pork industry. To date, numerous anti-CSFV monoclonal antibodies (mAbs) and their recognizing epitopes have been reported. However, few mAbs were systematically characterized for the capacity to differentiate field CSFV isolates from CSF vaccine strains, and the molecular basis associated with antigenic differences between vaccines and field isolates is still largely unknown. In the present study, recombinant CSFV structural glycoproteins E2 of both virulent and vaccine strains and E<jats:sup>rns<\/jats:sup> of vaccine strain were expressed using eukaryotic cells and murine mAbs generated against E2 and E<jats:sup>rns<\/jats:sup>. After serial screening and cloning of the hybridomas, the viral spectra of mAbs were respectively determined by indirect fluorescent antibody assay (IFA) using 108 CSFVs, followed by Western blot analysis using expressed glycoproteins of all CSFV sub-genotypes including vaccine strains. The antigenic structures recognized by these mAbs were characterized by epitope mapping using truncated, chimeric, and site-directed mutated E2 and E<jats:sup>rns<\/jats:sup> proteins. We have identified two vaccine-specific, one field isolate-specific, and two universal CSFV-specific mAbs and five novel conformational epitopes with critical amino acid (aa) motifs that are associated with these five mAbs: <jats:sup>213<\/jats:sup>EPD<jats:sup>215<\/jats:sup>, <jats:sup>271<\/jats:sup>RXGP<jats:sup>274<\/jats:sup>, and <jats:sup>37<\/jats:sup>LXLNDG<jats:sup>42<\/jats:sup> on E2 and <jats:sup>38<\/jats:sup>CKGVP<jats:sup>42<\/jats:sup>, W<jats:sup>81<\/jats:sup>, and D<jats:sup>100<\/jats:sup>\/V<jats:sup>107<\/jats:sup> on E<jats:sup>rns<\/jats:sup>. Particularly, E<jats:sup>213<\/jats:sup> of E2 is field isolate-specific, while N<jats:sup>40<\/jats:sup> of E2 and D<jats:sup>100<\/jats:sup>\/V<jats:sup>107<\/jats:sup> of E<jats:sup>rns<\/jats:sup> are vaccine strain-specific. Results from our study further indicate that N<jats:sup>40<\/jats:sup>D of E2 mutation in field strains was likely produced under positive selection associated with long-term mass vaccination, leading to CSFV evasion of host immune response. Taking together, this study provides new insights into the antigenic structure of CSFV E2 and E<jats:sup>rns<\/jats:sup> and the differentiating mAbs will contribute to the development of a diagnostic strategy to differentiate C-strain vaccination from natural infection (DIVA) of CSFV in terms of elimination of CSF in China.<\/jats:p>","DOI":"10.3389\/fimmu.2022.930631","type":"journal-article","created":{"date-parts":[[2022,7,19]],"date-time":"2022-07-19T04:38:38Z","timestamp":1658205518000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":9,"title":["Characterization of monoclonal antibodies that specifically differentiate field isolates from vaccine strains of classical swine fever 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