{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:16:37Z","timestamp":1787026597900,"version":"build-2736575974"},"reference-count":46,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,8,18]],"date-time":"2022-08-18T00:00:00Z","timestamp":1660780800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:p>\n                    Duck plague caused by duck plague virus (DPV) is a highly contagious disease that can cause serious morbidity and death in waterfowl such as ducks and geese, and bring huge economic losses to the duck industry. In this study, on the basis of the duck plague virus gC gene deletion strain CHv-\u0394gC, based on the duck plague virus bacterial artificial chromosome (BAC) platform in our laboratory, the gE gene was knocked out using the traceless deletion technology to obtain gC\/gE double gene deletion candidate vaccine strain CHv-\u0394gC\/gE. The double gene deletion strain (CHv-\u0394gC\/gE) constructed in this study has greatly weakened virulence, no pathogenicity to ducks, and stable genetic characteristics\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    . Ducks immunized with CHv-\u0394gC\/gE can produce neutralizing antibodies and ELISA antibody levels comparable to those of commercial duck plague attenuated vaccine immunization, and can resist 100 LD\n                    <jats:sub>50<\/jats:sub>\n                    CHv challenge of ducks, with good immune protection effect. It has the potential to be further developed into duck plague gC\/gE double gene deletion, marked attenuated vaccine.\n                  <\/jats:p>","DOI":"10.3389\/fimmu.2022.963009","type":"journal-article","created":{"date-parts":[[2022,8,18]],"date-time":"2022-08-18T02:18:47Z","timestamp":1660789127000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":16,"title":["Evaluation of safety and immunogenicity of duck-plague virus gC\/gE double gene 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