{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T02:35:34Z","timestamp":1788489334771,"version":"build-2803163510"},"reference-count":46,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,8,9]],"date-time":"2022-08-09T00:00:00Z","timestamp":1660003200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:p>\n                    <jats:italic>Flavobacterium psychrophilum<\/jats:italic>\n                    , the etiological agent of bacterial coldwater disease and rainbow trout fry syndrome, causes considerable losses in salmonid aquaculture globally. Systemic\n                    <jats:italic>F. psychrophilum<\/jats:italic>\n                    infections in rainbow trout (\n                    <jats:italic>Oncorhynchus mykiss<\/jats:italic>\n                    ) lead to a range of clinical signs, including ulcerative lesions in the skin and muscle and splenitis. Previous studies offered an integrative analysis of the skeletal muscle response to\n                    <jats:italic>F. psychrophilum<\/jats:italic>\n                    infection in rainbow trout. However, little is known about the molecular mechanism of immune response in the spleen, which is an important immune organ of rainbow trout. Here, we investigated the time-course splenic transcriptome profiles in uninfected rainbow trout (CK) and\n                    <jats:italic>F. psychrophilum<\/jats:italic>\n                    \u2013infected rainbow trout at day 3 and day 7 (D3, D7) by RNA-seq analyses. Among the 7,170 differentially expressed genes (DEGs) in the three comparisons (D3 vs. CK, D7 vs. CK, D3 vs. D7), 1,286 DEGs showed consistent upregulation or downregulation at D3 and D7 and were associated with pattern recognition, acute-phase response, complement cascade, chemokine and cytokine signaling, and apoptosis. The Real time quantitative PCR (RT-qPCR) analysis of eight DEGs confirmed the accuracy of the RNA-Sequencing (RNA-seq) data. Our results reflected a general process from pathogen recognition to inflammatory cytokine generation and delineated a putative Toll-like receptor signaling pathway in rainbow trout spleen, following\n                    <jats:italic>F. psychrophilum<\/jats:italic>\n                    infection. Taken together, these results provide new insights into the molecular mechanism of the immune response to\n                    <jats:italic>F. psychrophilum<\/jats:italic>\n                    infection and are a valuable resource for future research on the prevention and control of bacterial coldwater disease during salmon culture.\n                  <\/jats:p>","DOI":"10.3389\/fimmu.2022.965099","type":"journal-article","created":{"date-parts":[[2022,8,9]],"date-time":"2022-08-09T05:07:35Z","timestamp":1660021655000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":14,"title":["Time-course transcriptome analyses of spleen in rainbow trout (Oncorhynchus mykiss) post-Flavobacterium psychrophilum infection"],"prefix":"10.3389","volume":"13","author":[{"given":"Furong","family":"Deng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas 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