{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T21:31:41Z","timestamp":1785187901628,"version":"3.55.0"},"reference-count":55,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,10,5]],"date-time":"2022-10-05T00:00:00Z","timestamp":1664928000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Dysbiosis of the gut microbiota is closely related to chronic systemic inflammation and autoimmunity, playing an essential role in the pathogenesis of primary Sj\u00f6gren\u2019s syndrome (pSS). Abnormalities in the proportions of blood T lymphocyte subtype, that is Th17\/Treg, were detected in pSS patients. We aimed to determine the associations between gut microbiota and Th17\/Treg in pSS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>98 pSS patients and 105 healthy controls (NC) were enrolled between Dec 1, 2018, and Aug 31, 2019. The baseline information and clinical parameters on pSS patients and healthy controls were collected. 16S rRNA sequencing was performed to characterize the gut microbiome and identify gut microbes that are differentially abundant between patients and healthy controls. Lastly, associations between relative abundances of specific bacterial taxa in the gut and clinical outcome parameters were evaluated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Patients with pSS show decreased gut microbial diversity and richness, decreased abundance of butyrate producing bacteria, such as <jats:italic>Roseburia<\/jats:italic> and <jats:italic>Coprococcus<\/jats:italic>, and increased abundance of other taxa, such as <jats:italic>Eubacterium rectale<\/jats:italic> and <jats:italic>Roseburia inulinivorans<\/jats:italic>. These bacteria are enriched with functions related to glycolytic and lipogenic, energy, substance, galactose, pentose metabolism pathways and glucuronate interconversions, decreased with functions related to peptidoglycan biosynthesis, pyrimidine metabolism pathways. An integrative analysis identified pSS-related specific bacterial taxa in the gut, for which the abundance of <jats:italic>Eubacterium rectale<\/jats:italic> is negatively correlated with Th17\/Treg. Furthermore, the pathways of biosynthesis of secondary metabolites, biosynthesis of amino acids, peptidoglycan biosynthesis and pyrimidine, galactose, pentose, microbial metabolism in diverse environments, glyoxylate and dicarboxylate metabolism are associated with Treg or Th17\/Treg.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Primary Sj\u00f6gren\u2019s syndrome could lead to decreased gut microbial diversity and richness of intestinal flora in patients. The proportions of Th17 and Treg cells induced by microbiota were predictive pSS manifestations and accounted for the pSS severity.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fimmu.2022.974648","type":"journal-article","created":{"date-parts":[[2022,10,5]],"date-time":"2022-10-05T05:35:32Z","timestamp":1664948132000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":46,"title":["Th17 cells in primary Sj\u00f6gren\u2019s syndrome negatively correlate with increased Roseburia and 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