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However, the unique role of the immune environment in tumor progression for relapsed chemo-resistant patients remains elusive. In single-cell resolution, we characterized a comprehensive multi-dimensional cellular and immunological atlas from tumor, ascites, and peripheral blood of a chemo-resistant patient at different stages of treatment. Our results highlight a role in recurrence and chemoresistance of the immunosuppressive microenvironment in ascites, including MDSC-like myeloid and hypo-metabolic \u03b3\u03b4T cells, and of peripheral CD8<jats:sup>+<\/jats:sup> effector T cells with chemotherapy-induced senescent\/exhaustive. Importantly, paired TCR\/BCR sequencing demonstrated relative conservation of TCR clonal expansion in hyper-expanded CD8<jats:sup>+<\/jats:sup> T cells and extensive BCR clonal expansion without usage bias of V(D)J genes after chemotherapy. Thus, our study suggests strategies for ameliorating chemotherapy-induced immune impairment to improve the clinical outcome of HGSOC.<\/jats:p>","DOI":"10.3389\/fimmu.2022.985187","type":"journal-article","created":{"date-parts":[[2022,9,28]],"date-time":"2022-09-28T08:22:35Z","timestamp":1664353355000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":34,"title":["Single-cell sequencing reveals effects of chemotherapy on the immune landscape and TCR\/BCR clonal expansion in a relapsed ovarian cancer 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