{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T17:35:36Z","timestamp":1786988136230,"version":"build-2736575974"},"reference-count":55,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,5,19]],"date-time":"2023-05-19T00:00:00Z","timestamp":1684454400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007765","name":"Shenyang Science and Technology Bureau","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007765","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p>Myocardial injury is a common complication in patients with endotoxaemia\/sepsis, especially in children. Moreover, it develops through an unclear pathophysiological mechanism, and effective therapies are lacking. Recently, RNA modification, particularly <jats:italic>N<\/jats:italic><jats:sup>6<\/jats:sup>-methyladenosine (m<jats:sup>6<\/jats:sup>A) modification, has been found to be involved in various physiological processes and to play important roles in many diseases. However, the role of m<jats:sup>6<\/jats:sup>A modification in endotoxaemia\/sepsis-induced myocardial injury is still in its infancy. Therefore, we attempted to construct the m<jats:sup>6<\/jats:sup>A modification map of myocardial injury in a rat model treated by lipopolysaccharide (LPS) and explore the role of m<jats:sup>6<\/jats:sup>A modification in LPS-induced myocardial injury.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>Myocardial injury adolescent rat model was constructed by intraperitoneal injection of LPS. m<jats:sup>6<\/jats:sup>A RNA Methylation Quantification Kit was used to detect overall level of m<jats:sup>6<\/jats:sup>A modification in rat cardiac tissue. m<jats:sup>6<\/jats:sup>A-specific methylated RNA immunoprecipitation followed by high-throughput sequencing (MeRIP-seq) and RNA sequencing (RNA-seq) were conducted to identify the altered m<jats:sup>6<\/jats:sup>A-modified genes and differentially expressed genes in cardiac tissue of rats treated by LPS and control rats (6 versus. 6). Bioinformatics was used to analyze the functions of differentially m<jats:sup>6<\/jats:sup>A modified genes, differentially expressed genes, and genes with both differential m<jats:sup>6<\/jats:sup>A modification and differential expression. qPCR was used to detect expression of m<jats:sup>6<\/jats:sup>A modification related enzymes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Result<\/jats:title><jats:p>We found that the overall level of m<jats:sup>6<\/jats:sup>A modification in cardiac tissue of the LPS group was up-regulated compared with that of the control group. MeRIP-seq and RNA-seq results showed that genes with differential m<jats:sup>6<\/jats:sup>A modification, genes with differential expression and genes with both differential m<jats:sup>6<\/jats:sup>A modification and differential expression were closely associated with inflammatory responses and apoptosis. In addition, we found that m<jats:sup>6<\/jats:sup>A-related enzymes (Mettl16, Rbm15, Fto, Ythdc2 and Hnrnpg) were differentially expressed in the LPS group versus. the control group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>m<jats:sup>6<\/jats:sup>A modification is involved in the pathogenesis process of LPS-induced myocardial injury, possibly through the regulation of inflammatory response and apoptosis-related pathways. These results provide valuable information regarding the potential pathogenic mechanisms underlying LPS-induced myocardial injury.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fimmu.2023.1122317","type":"journal-article","created":{"date-parts":[[2023,5,19]],"date-time":"2023-05-19T06:14:40Z","timestamp":1684476880000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":13,"title":["Transcriptome-wide identification of altered RNA m6A profiles in cardiac tissue of rats with LPS-induced myocardial 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