{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:15:21Z","timestamp":1787026521695,"version":"build-2736575974"},"reference-count":59,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,2,16]],"date-time":"2023-02-16T00:00:00Z","timestamp":1676505600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["BA4730\/3-2"],"award-info":[{"award-number":["BA4730\/3-2"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"\n Streptococcus suis<\/jats:italic>\n (\n S. suis<\/jats:italic>\n ) is an important porcine pathogen, causing severe disease like meningitis and septicemia primarily in piglets. Previous work showed that the IgM-degrading enzyme of\n S. suis<\/jats:italic>\n (Ide\n \n Ssuis<\/jats:sub>\n <\/jats:italic>\n ) specifically cleaves soluble porcine IgM and is involved in complement evasion. The objective of this study was to investigate Ide\n \n Ssuis<\/jats:sub>\n <\/jats:italic>\n cleavage of the IgM B cell receptor and subsequent changes in B cell receptor mediated signaling. Flow cytometry analysis revealed cleavage of the IgM B cell receptor by recombinant (r) Ide\n \n Ssuis<\/jats:sub>\n <\/jats:italic>\n _homologue as well as Ide\n \n Ssuis<\/jats:sub>\n <\/jats:italic>\n derived from culture supernatants of\n S. suis<\/jats:italic>\n serotype 2 on porcine PBMCs and mandibular lymph node cells. Point-mutated rIde\n \n Ssuis<\/jats:sub>\n <\/jats:italic>\n _homologue_C195S did not cleave the IgM B cell receptor. After receptor cleavage by rIde\n \n Ssuis<\/jats:sub>\n <\/jats:italic>\n _homologue, it took at least 20 h for mandibular lymph node cells to restore the IgM B cell receptor to levels comparable to cells previously treated with rIde\n \n Ssuis<\/jats:sub>\n <\/jats:italic>\n _homologue_C195S. B cell receptor mediated signaling after specific stimulation\n via<\/jats:italic>\n the F(ab\u2019)\n 2<\/jats:sub>\n portion was significantly inhibited by rIde\n \n Ssuis<\/jats:sub>\n <\/jats:italic>\n _homologue receptor cleavage in IgM\n +<\/jats:sup>\n B cells, but not in IgG\n +<\/jats:sup>\n B cells. Within IgM\n +<\/jats:sup>\n cells, CD21\n +<\/jats:sup>\n B2 cells and CD21\n -<\/jats:sup>\n B1-like cells were equally impaired in their signaling capacity upon rIde\n \n Ssuis<\/jats:sub>\n <\/jats:italic>\n _homologue B cell receptor cleavage. In comparison, intracellular B cell receptor independent stimulation with tyrosine phosphatase inhibitor pervanadate increased signaling in all investigated B cell types. In conclusion, this study demonstrates Ide\n \n Ssuis<\/jats:sub>\n <\/jats:italic>\n cleavage efficacy on the IgM B cell receptor and its consequences for B cell signaling.\n <\/jats:p>","DOI":"10.3389\/fimmu.2023.1122808","type":"journal-article","created":{"date-parts":[[2023,2,16]],"date-time":"2023-02-16T01:02:44Z","timestamp":1676509364000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":10,"title":["Immunoglobulin M-degrading enzyme of Streptococcus suis (IdeSsuis) impairs porcine B cell signaling"],"prefix":"10.3389","volume":"14","author":[{"given":"Annika 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