{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T14:29:47Z","timestamp":1792506587132,"version":"4.5.0"},"reference-count":46,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,4,11]],"date-time":"2023-04-11T00:00:00Z","timestamp":1681171200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:p><jats:italic>Candida auris<\/jats:italic>, an emerging multi-drug resistant fungal pathogen, causes invasive infections in humans. The factors regulating the colonization of <jats:italic>C. auris<\/jats:italic> in host niches are not well understood. In this study, we examined the effect of antibiotic-induced gut dysbiosis on <jats:italic>C. auris<\/jats:italic> intestinal colonization, dissemination, microbiome composition and the mucosal immune response. Our results indicate that mice treated with cefoperazone alone had a significant increase in <jats:italic>C. auris<\/jats:italic> intestinal colonization compared to untreated control groups. A significant increase in the dissemination of <jats:italic>C. auris<\/jats:italic> from the intestine to internal organs was observed in antibiotic-treated immunosuppressed mice. Intestinal colonization of <jats:italic>C. auris<\/jats:italic> alters the microbiome composition of antibiotic-treated mice. Relative abundance of firmicutes members mainly <jats:italic>Clostridiales<\/jats:italic> and <jats:italic>Paenibacillus<\/jats:italic> were considerably increased in the cefoperazone-treated mice infected with <jats:italic>C. auris<\/jats:italic> compared to cefoperazone-treated uninfected mice. Next, we examined the mucosal immune response of <jats:italic>C. auris<\/jats:italic> infected mice and compared the results with <jats:italic>Candida albicans<\/jats:italic> infection. The number of CD11b+ CX3CR1+ macrophages was significantly decreased in the intestine of <jats:italic>C. auris<\/jats:italic> infected mice when compared to <jats:italic>C. albicans<\/jats:italic> infection. On the other hand, both <jats:italic>C. auris<\/jats:italic> and <jats:italic>C. albicans<\/jats:italic> infected mice had a comparable increase of the number of Th17 and Th22 cells in the intestine. A significant increase in <jats:italic>Candida<\/jats:italic>-specific IgA was observed in the serum of <jats:italic>C. auris<\/jats:italic> but not in the <jats:italic>C. albicans<\/jats:italic> infected mice. Taken together, treatment with broad-spectrum antibiotic increased the colonization and dissemination of <jats:italic>C. auris<\/jats:italic> from the intestine. Furthermore, findings from this study for the first time revealed the microbiome composition, innate and adaptive cellular immune response to intestinal infection with <jats:italic>C. auris<\/jats:italic>.<\/jats:p>","DOI":"10.3389\/fimmu.2023.1123200","type":"journal-article","created":{"date-parts":[[2023,4,11]],"date-time":"2023-04-11T04:57:51Z","timestamp":1681189071000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":17,"title":["Intestinal colonization with Candida auris and mucosal immune response in mice treated with cefoperazone oral antibiotic"],"prefix":"10.3389","volume":"14","author":[{"given":"Diprasom","family":"Das","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harm","family":"HogenEsch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shankar","family":"Thangamani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,4,11]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"e1008921","DOI":"10.1371\/journal.ppat.1008921","article-title":"Candida auris: Epidemiology, biology, antifungal resistance, and virulence","volume":"16","author":"Du","year":"2020","journal-title":"PloS Pathog"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2022.102208","article-title":"The molecular and genetic basis of antifungal resistance in the emerging fungal pathogen candida 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