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Immunol."],"abstract":"<jats:p>Helminth-induced eosinophils accumulate around the parasite at the site of infection, or in parasite-damaged tissues well after the helminth has left the site. The role of helminth-elicited eosinophils in mediating parasite control is complex. While they may contribute to direct parasite-killing and tissue repair, their involvement in long-term immunopathogenesis is a concern. In allergic Siglec-F<jats:sup>hi<\/jats:sup>CD101<jats:sup>hi<\/jats:sup>, eosinophils are associated with pathology. Research has not shown if equivalent subpopulations of eosinophils are a feature of helminth infection. In this study, we demonstrate that lung migration of rodent hookworm <jats:italic>Nippostrongylus brasiliensis<\/jats:italic> (<jats:italic>Nb<\/jats:italic>) results in a long-term expansion of distinct Siglec-F<jats:sup>hi<\/jats:sup>CD101<jats:sup>hi<\/jats:sup> eosinophil subpopulations. <jats:italic>Nb<\/jats:italic>-elevated eosinophil populations in the bone marrow and circulation did not present this phenotype. Siglec-F<jats:sup>hi<\/jats:sup>CD101<jats:sup>hi<\/jats:sup> lung eosinophils exhibited an activated morphology including nuclei hyper-segmentation and cytoplasm degranulation. Recruitment of ST2<jats:sup>+<\/jats:sup> ILC2s and not CD4<jats:sup>+<\/jats:sup> T cells to the lungs was associated with the expansion of Siglec-F<jats:sup>hi<\/jats:sup>CD101<jats:sup>hi<\/jats:sup> eosinophils. This data identifies a morphologically distinct and persistent subset of Siglec-F<jats:sup>hi<\/jats:sup>CD101<jats:sup>hi<\/jats:sup> lung eosinophils induced following <jats:italic>Nb<\/jats:italic> infection. These eosinophils may contribute to long-term pathology following helminth infection.<\/jats:p>","DOI":"10.3389\/fimmu.2023.1170807","type":"journal-article","created":{"date-parts":[[2023,5,12]],"date-time":"2023-05-12T07:54:32Z","timestamp":1683878072000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":12,"title":["Induction of Siglec-FhiCD101hi eosinophils in the lungs following murine hookworm Nippostrongylus brasiliensis infection"],"prefix":"10.3389","volume":"14","author":[{"given":"Alisha","family":"Chetty","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew G.","family":"Darby","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jamie","family":"Pillaye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A'ishah","family":"Taliep","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam F.","family":"Cunningham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew K.","family":"O\u2019Shea","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gnatoulma","family":"Katawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura E.","family":"Layland","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel","family":"Ritter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William G. 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