{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T20:44:07Z","timestamp":1772311447768,"version":"3.50.1"},"reference-count":111,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,7,27]],"date-time":"2023-07-27T00:00:00Z","timestamp":1690416000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004359","name":"Vetenskapsr\u00e5det","doi-asserted-by":"publisher","award":["2021-06602, 2022-00520, 2018-0241"],"award-info":[{"award-number":["2021-06602, 2022-00520, 2018-0241"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003748","name":"Svenska S\u00e4llskapet f\u00f6r Medicinsk Forskning","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003748","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:p>Dendritic cells and macrophages are integral parts of the innate immune system and gatekeepers against infection. The protozoan pathogen, <jats:italic>Toxoplasma gondii<\/jats:italic>, is known to hijack host immune cells and modulate their immune response, making it a compelling model to study host-pathogen interactions. Here we utilize single cell Dual RNA-seq to parse out heterogeneous transcription of mouse bone marrow-derived dendritic cells (BMDCs) infected with two distinct genotypes of <jats:italic>T. gondii<\/jats:italic> parasites, over multiple time points post infection. We show that the BMDCs elicit differential responses towards <jats:italic>T. gondii<\/jats:italic> infection and that the two parasite lineages distinctly manipulate subpopulations of infected BMDCs. Co-expression networks define host and parasite genes, with implications for modulation of host immunity. Integrative analysis validates previously established immune pathways and additionally, suggests novel candidate genes involved in host-pathogen interactions. Altogether, this study provides a comprehensive resource for characterizing host-pathogen interplay at high-resolution.<\/jats:p>","DOI":"10.3389\/fimmu.2023.1224591","type":"journal-article","created":{"date-parts":[[2023,7,28]],"date-time":"2023-07-28T01:08:52Z","timestamp":1690506532000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":5,"title":["scDual-Seq of Toxoplasma gondii-infected mouse BMDCs reveals heterogeneity and differential infection dynamics"],"prefix":"10.3389","volume":"14","author":[{"given":"Franziska","family":"Hildebrandt","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mubasher","family":"Mohammed","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexis","family":"Dziedziech","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amol K.","family":"Bhandage","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna-Maria","family":"Divne","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fredrik","family":"Barren\u00e4s","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Barragan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johan","family":"Henriksson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johan","family":"Ankarklev","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2023,7,27]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06246","article-title":"Recognition of microorganisms and activation of the immune response","volume":"449","author":"Medzhitov","year":"2007","journal-title":"Nature"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1004-971","article-title":"The interface between innate and adaptive immunity","volume":"5","author":"Hoebe","year":"2004","journal-title":"Nat Immunol"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.02.041","article-title":"Toll-like receptors and the control of immunity","volume":"180","author":"Fitzgerald","year":"2020","journal-title":"Cell"},{"key":"B4","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1186\/s42269-019-0227-2","article-title":"Toll-like receptors activation, signaling, and targeting: an overview","volume":"43","author":"El-Zayat","year":"2019","journal-title":"Bull Natl Res Cent"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1038\/nri3712","article-title":"Dendritic cells, monocytes and macrophages: a unified nomenclature based on ontogeny","volume":"14","author":"Guilliams","year":"2014","journal-title":"Nat Rev Immunol"},{"key":"B6","doi-asserted-by":"publisher","first-page":"E92","DOI":"10.3390\/ijms19010092","article-title":"The phagocytic function of macrophage-enforcing innate immunity and tissue homeostasis","volume":"19","author":"Hirayama","year":"2017","journal-title":"Int J Mol Sci"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1038\/nri3683","article-title":"The origins and functions of dendritic cells and macrophages in the skin","volume":"14","author":"Malissen","year":"2014","journal-title":"Nat Rev Immunol"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.3390\/cells9010161","article-title":"Pathogens menTORing macrophages and dendritic cells: manipulation of mTOR and cellular metabolism to promote immune escape","volume":"9","author":"Nouwen","year":"2020","journal-title":"Cells"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2015.05.018","article-title":"GM-CSF mouse bone marrow cultures comprise a heterogeneous population of CD11c+MHCII+ Macrophages and dendritic cells","volume":"42","author":"Helft","year":"2015","journal-title":"Immunity"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1038\/nature12172","article-title":"Single-cell transcriptomics reveals bimodality in expression and splicing in immune cells","volume":"498","author":"Shalek","year":"2013","journal-title":"Nature"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4922(02)02292-4","article-title":"Sexual differentiation and sex determination in the Apicomplexa","volume":"18","author":"Smith","year":"2002","journal-title":"Trends Parasitol"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0090203","article-title":"Toxoplasmosis\u2013a global threat. Correlation of latent toxoplasmosis with specific disease burden in a set of 88 countries","volume":"9","author":"Flegr","year":"2014","journal-title":"PloS One"},{"key":"B13","volume-title":"Toxoplasmosis of animals and humans","author":"Dubey","year":"2010"},{"key":"B14","article-title":"Detection of toxoplasma gondii in acute and chronic phases of infection in immunocompromised patients and pregnant women with real-time PCR assay using taqMan fluorescent probe","volume":"13","author":"Mousavi","year":"2018","journal-title":"Iran J Parasitol"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0020013","article-title":"Cytoskeletal components of an invasion machine\u2014The apical complex of toxoplasma gondii","volume":"2","author":"Hu","year":"2006","journal-title":"PloS Pathog"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.18-0344","article-title":"How does Toxoplama gondii invade host cells","volume":"80","author":"Kato","year":"2018","journal-title":"J Vet Med Sci"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20020258","article-title":"Transepithelial migration of toxoplasma gondii is linked to parasite motility and virulence","volume":"195","author":"Barragan","year":"2002","journal-title":"J Exp Med"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2019.00046","article-title":"From entry to early dissemination\u2014Toxoplasma gondii\u2019s initial encounter with its host. Front. Cell. Infect","volume":"9","author":"Delgado Betancourt","year":"2019","journal-title":"Microbiol"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2019.00073","article-title":"Toxoplasma-induced hypermigration of primary cortical microglia implicates GABAergic signaling","volume":"9","author":"Bhandage","year":"2019","journal-title":"Front Cell Infect Microbiol"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2006.00735.x","article-title":"Induction of dendritic cell migration upon Toxoplasma gondii infection potentiates parasite dissemination","volume":"8","author":"Lambert","year":"2006","journal-title":"Cell Microbiol"},{"key":"B21","doi-asserted-by":"publisher","first-page":"1570","DOI":"10.1016\/j.chom.2022.10.001","article-title":"The Toxoplasma effector GRA28 promotes parasite dissemination by inducing dendritic cell-like migratory properties in infected macrophages","volume":"30","author":"ten Hoeve","year":"2022","journal-title":"Cell Host Microbe"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1038\/icb.2014.106","article-title":"Toxoplasma gondii -infected natural killer cells display a hypermotility phenotype in vivo","volume":"93","author":"Ueno","year":"2015","journal-title":"Immunol Cell Biol"},{"key":"B23","doi-asserted-by":"publisher","first-page":"292","DOI":"10.1111\/j.1462-5822.2009.01417.x","article-title":"Modelling parasite dissemination: host cell subversion and immune evasion by Toxoplasma gondii","volume":"12","author":"Lambert","year":"2010","journal-title":"Cell Microbiol"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.62.081307.162925","article-title":"Population structure of Toxoplasma gondii : clonal expansion driven by infrequent recombination and selective sweeps","volume":"62","author":"Sibley","year":"2008","journal-title":"Annu Rev Microbiol"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/172.6.1561","article-title":"Toxoplasma gondii comprises three clonal lineages: correlation of parasite genotype with human disease. The","volume":"172","author":"Howe","year":"1995","journal-title":"J Infect Dis"},{"key":"B26","doi-asserted-by":"publisher","first-page":"R53","DOI":"10.1186\/gb-2009-10-5-r53","article-title":"Whole genome sequencing of a natural recombinant Toxoplasma gondii strain reveals chromosome sorting and local allelic variants","volume":"10","author":"Bontell","year":"2009","journal-title":"Genome Biol"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1038\/359082a0","article-title":"Virulent strains of Toxoplasma gondii comprise a single clonal lineage","volume":"359","author":"Sibley","year":"1992","journal-title":"Nature"},{"key":"B28","doi-asserted-by":"publisher","first-page":"e0139104","DOI":"10.1371\/journal.pone.0139104","article-title":"Infection by toxoplasma gondii induces amoeboid-like migration of dendritic cells in a three-dimensional collagen matrix","volume":"10","author":"Kanatani","year":"2015","journal-title":"PloS One"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2011.04.015","article-title":"Toxoplasma polymorphic effectors determine macrophage polarization and intestinal inflammation","volume":"9","author":"Jensen","year":"2011","journal-title":"Cell Host Microbe"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003779","article-title":"Transcriptional analysis of murine macrophages infected with different toxoplasma strains identifies novel regulation of host signaling pathways","volume":"9","author":"Melo","year":"2013","journal-title":"PloS Pathog"},{"key":"B31","doi-asserted-by":"publisher","first-page":"130","DOI":"10.1186\/s12864-021-07437-0","article-title":"Transcriptomics analysis of Toxoplasma gondii-infected mouse macrophages reveals coding and noncoding signatures in the presence and absence of MyD88","volume":"22","author":"Menard","year":"2021","journal-title":"BMC Genomics"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-15-806","article-title":"Dual transcriptional profiling of mice and Toxoplasma gondii during acute and chronic infection","volume":"15","author":"Pittman","year":"2014","journal-title":"BMC Genomics"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1010502","article-title":"NF kappa B regulator Bcl3 controls development and function of classical dendritic cells required for resistance to Toxoplasma gondii","volume":"18","author":"Guha","year":"2022","journal-title":"PloS Pathog"},{"key":"B34","doi-asserted-by":"publisher","first-page":"200","DOI":"10.1186\/s13059-017-1340-x","article-title":"scDual-Seq: mapping the gene regulatory program of Salmonella infection by host and pathogen single-cell RNA-sequencing","volume":"18","author":"Avital","year":"2017","journal-title":"Genome Biol"},{"key":"B35","doi-asserted-by":"publisher","first-page":"1607","DOI":"10.1038\/s41467-019-09599-8","article-title":"Coordinated host-pathogen transcriptional dynamics revealed using sorted subpopulations and single macrophages infected with Candida albicans","volume":"10","author":"Mu\u00f1oz","year":"2019","journal-title":"Nat Commun"},{"key":"B36","doi-asserted-by":"publisher","first-page":"21047","DOI":"10.1038\/s41598-020-78250-0","article-title":"Single-cell RNA-seq reveals CD16- monocytes as key regulators of human monocyte transcriptional response to Toxoplasma","volume":"10","author":"Patir","year":"2020","journal-title":"Sci Rep"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2022.848693","article-title":"Single cell transcriptomes of In Vitro bradyzoite infected cells reveals toxoplasma gondii stage dependent host cell alterations","volume":"12","author":"Sugi","year":"2022","journal-title":"Front Cell Infect Microbiol"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.11.2.267","article-title":"Structures of Toxoplasma gondii tachyzoites, bradyzoites, and sporozoites and biology and development of tissue cysts","volume":"11","author":"Dubey","year":"1998","journal-title":"Clin Microbiol Rev"},{"key":"B39","doi-asserted-by":"publisher","first-page":"689","DOI":"10.1186\/s13104-017-3005-y","article-title":"Selection of a suitable reference gene for quantitative gene expression in mouse lymph nodes after vaccination","volume":"10","author":"Mosley","year":"2017","journal-title":"BMC Res Notes"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.01089","article-title":"Molecular analysis of a short-term model of \u03b2-glucans-trained immunity highlights the accessory contribution of GM-CSF in priming mouse macrophages response","volume":"8","author":"Walachowski","year":"2017","journal-title":"Front Immunol"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0036465","article-title":"Identification and validation of ifit1 as an important innate immune bottleneck","volume":"7","author":"McDermott","year":"2012","journal-title":"PloS One"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2019.00349","article-title":"Sustained Egr-1 Response via p38 MAP Kinase Signaling Modulates Early Immune Responses of Dendritic Cells Parasitized by Toxoplasma gondii","volume":"9","author":"Ten Hoeve","year":"2019","journal-title":"Front Cell Infect Microbiol"},{"key":"B43","doi-asserted-by":"crossref","DOI":"10.1016\/B978-0-12-396481-6.00015-5","article-title":"Chapter 15 - Bradyzoite Development","volume-title":"Toxoplasma Gondii","author":"Knoll","year":"2014"},{"key":"B44","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1016\/j.chom.2008.01.001","article-title":"Toxoplasma profilin is essential for host cell invasion and TLR11-dependent induction of an interleukin-12 response","volume":"3","author":"Plattner","year":"2008","journal-title":"Cell Host Microbe"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0020084","article-title":"Toxoplasma MIC2 is a major determinant of invasion and virulence","volume":"2","author":"Huynh","year":"2006","journal-title":"PloS Pathog"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-7-180","article-title":"Research progress on surface antigen 1 (SAG1) of Toxoplasma gondii","volume":"7","author":"Wang","year":"2014","journal-title":"Parasit Vectors"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1038\/s41423-020-0489-5","article-title":"ACOD1 in immunometabolism and disease","volume":"17","author":"Wu","year":"2020","journal-title":"Cell Mol Immunol"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.00861","article-title":"Macrophage inducible C-type lectin as a multifunctional player in immunity","volume":"8","author":"Patin","year":"2017","journal-title":"Front Immunol"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2013.00032","article-title":"Role of CD14 in host protection against infections and in metabolism regulation","volume":"3","author":"Zanoni","year":"2013","journal-title":"Front Cell Infect Microbiol"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-006-0108-z","article-title":"ABCA1, from pathology to membrane function","volume":"453","author":"Zarubica","year":"2007","journal-title":"Pflugers Arch - Eur J Physiol"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00255-13","article-title":"Human innate immunity to toxoplasma gondii is mediated by host caspase-1 and ASC and parasite GRA15","volume":"4","author":"Gov","year":"2013","journal-title":"mBio"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20160006","article-title":"Genetic functions of the NAIP family of inflammasome receptors for bacterial ligands in mice","volume":"213","author":"Zhao","year":"2016","journal-title":"J Exp Med"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.39887","article-title":"Characterization of a Toxoplasma effector uncovers an alternative GSK3\/\u03b2-catenin-regulatory pathway of inflammation","volume":"7","author":"He","year":"2018","journal-title":"eLife"},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2007.08.015","article-title":"Disruption of E-cadherin-mediated adhesion induces a functionally distinct pathway of dendritic cell maturation","volume":"27","author":"Jiang","year":"2007","journal-title":"Immunity"},{"key":"B55","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1007\/s40588-019-0114-5","article-title":"Toxoplasma: immunity and pathogenesis","volume":"6","author":"Khan","year":"2019","journal-title":"Curr Clin Micro Rpt"},{"key":"B56","doi-asserted-by":"publisher","first-page":"28","DOI":"10.1016\/j.imbio.2011.08.008","article-title":"Cell invasion and strain dependent induction of suppressor of cytokine signaling-1 by Toxoplasma gondii","volume":"217","author":"Stutz","year":"2012","journal-title":"Immunobiology"},{"key":"B57","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2015.07.017","article-title":"Inflammatory signaling by NOD-RIPK2 is inhibited by clinically relevant type II kinase inhibitors","volume":"22","author":"Canning","year":"2015","journal-title":"Chem Biol"},{"key":"B58","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1155\/2010\/828951","article-title":"Peroxisome proliferator-activated receptor (PPAR): balance for survival in parasitic infections","volume":"2010","author":"Chan","year":"2010","journal-title":"J Biomed Biotechnol"},{"key":"B59","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0177161","article-title":"Structures of the inactive and active states of RIP2 kinase inform on the mechanism of activation","volume":"12","author":"Pellegrini","year":"2017","journal-title":"PloS One"},{"key":"B60","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2008.11.014","article-title":"Induction of mitotic S-phase of host and neighboring cells by Toxoplasma gondii enhances parasite invasion","volume":"164","author":"Lavine","year":"2009","journal-title":"Mol Biochem Parasitol"},{"key":"B61","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2008.01117.x","article-title":"Infection with Toxoplasma gondii results in dysregulation of the host cell cycle","volume":"10","author":"Molestina","year":"2008","journal-title":"Cell Microbiol"},{"key":"B62","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0504934102","article-title":"Knockout mice reveal a tumor suppressor function for Testin","volume":"102","author":"Drusco","year":"2005","journal-title":"Proc Natl Acad Sci USA"},{"key":"B63","doi-asserted-by":"publisher","first-page":"448","DOI":"10.1038\/s42003-020-01161-3","article-title":"FAM46C\/TENT5C functions as a tumor suppressor through inhibition of Plk4 activity","volume":"3","author":"Kazazian","year":"2020","journal-title":"Commun Biol"},{"key":"B64","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0012354","article-title":"Coordinated Progression through Two Subtranscriptomes Underlies the Tachyzoite Cycle of Toxoplasma gondii","volume":"5","author":"Behnke","year":"2010","journal-title":"PloS One"},{"key":"B65","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2003.12.004","article-title":"The SRS superfamily of Toxoplasma surface proteins","volume":"34","author":"Jung","year":"2004","journal-title":"Int J Parasitol"},{"key":"B66","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2010.01509.x","article-title":"Toxoplasma gondii protease TgSUB1 is required for cell surface processing of micronemal adhesive complexes and efficient adhesion of tachyzoites: TgSUB1 microneme protein processing","volume":"12","author":"Lagal","year":"2010","journal-title":"Cell Microbiol"},{"key":"B67","doi-asserted-by":"publisher","first-page":"752","DOI":"10.1016\/j.chom.2020.09.011","article-title":"A Comprehensive Subcellular Atlas of the Toxoplasma Proteome via hyperLOPIT Provides Spatial Context for Protein Functions","volume":"28","author":"Barylyuk","year":"2020","journal-title":"Cell Host Microbe"},{"key":"B68","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2007.09.013","article-title":"Rhoptries: an arsenal of secreted virulence factors","volume":"10","author":"Bradley","year":"2007","journal-title":"Curr Opin Microbiol"},{"key":"B69","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12733","article-title":"The Plasmodium rhoptry associated protein complex is important for parasitophorous vacuole membrane structure and intraerythrocytic parasite growth","volume":"19","author":"Ghosh","year":"2017","journal-title":"Cell Microbiol"},{"key":"B70","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2019.00413","article-title":"Influence of two major toxoplasma gondii virulence factors (ROP16 and ROP18) on the immune response of peripheral blood mononuclear cells to human toxoplasmosis infection","volume":"9","author":"Hern\u00e1ndez-de-los-R\u00edos","year":"2019","journal-title":"Front Cell Infect Microbiol"},{"key":"B71","doi-asserted-by":"publisher","first-page":"495","DOI":"10.1016\/j.cels.2020.10.001","article-title":"Pairwise stimulations of pathogen-sensing pathways predict immune responses to multi-adjuvant combinations","volume":"11","author":"Pandey","year":"2020","journal-title":"Cell Syst"},{"key":"B72","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-18991-8","article-title":"Genome-wide screens identify Toxoplasma gondii determinants of parasite fitness in IFN\u03b3-activated murine macrophages","volume":"11","author":"Wang","year":"2020","journal-title":"Nat Commun"},{"key":"B73","doi-asserted-by":"publisher","DOI":"10.1111\/exd.12603","article-title":"CpGB DNA activates dermal macrophages and specifically recruits inflammatory monocytes into the skin","volume":"24","author":"Mathes","year":"2015","journal-title":"Exp Dermatol"},{"key":"B74","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1038\/nri1745","article-title":"Dectin-1: a signalling non-TLR pattern-recognition receptor","volume":"6","author":"Brown","year":"2006","journal-title":"Nat Rev Immunol"},{"key":"B75","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2016.09.009","article-title":"Association of a network of interferon-stimulated genes with a locus encoding a negative regulator of non-conventional IKK kinases and IFNB1","volume":"17","author":"Jeidane","year":"2016","journal-title":"Cell Rep"},{"key":"B76","doi-asserted-by":"publisher","DOI":"10.1038\/s41423-019-0278-1","article-title":"Essential roles of S100A10 in Toll-like receptor signaling and immunity to infection","volume":"17","author":"Lou","year":"2020","journal-title":"Cell Mol Immunol"},{"key":"B77","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2020.1843253","article-title":"Extracellular SQSTM1 as an inflammatory mediator","volume":"16","author":"Zou","year":"2020","journal-title":"Autophagy"},{"key":"B78","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0139733","article-title":"Endocytosis\u2212Mediated invasion and pathogenicity of streptococcus agalactiae in rat cardiomyocyte (H9C2)","volume":"10","author":"Pooja","year":"2015","journal-title":"PloS One"},{"key":"B79","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2021.109584","article-title":"The macrophage-derived protein PTMA induces filamentation of the human fungal pathogen Candida albicans","volume":"36","author":"Case","year":"2021","journal-title":"Cell Rep"},{"key":"B80","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.01764","article-title":"Tolerance through education: how tolerogenic dendritic cells shape immunity","volume":"8","author":"Domogalla","year":"2017","journal-title":"Front Immunol"},{"key":"B81","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2011.01015.x","article-title":"Dendritic cell control of tolerogenic responses","volume":"241","author":"Manicassamy","year":"2011","journal-title":"Immunol Rev"},{"key":"B82","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2015.05.030","article-title":"GM-CSF: An immune modulatory cytokine that can suppress autoimmunity","volume":"75","author":"Bhattacharya","year":"2015","journal-title":"Cytokine"},{"key":"B83","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1186\/s13024-018-0255-7","article-title":"Tolerogenic bone marrow-derived dendritic cells induce neuroprotective regulatory T cells in a model of Parkinson\u2019s disease","volume":"13","author":"Schutt","year":"2018","journal-title":"Mol Neurodegeneration"},{"key":"B84","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.1212622","article-title":"C-type lectin receptor Clec4d plays a protective role in resolution of Gram-negative pneumonia","volume":"94","author":"Steichen","year":"2013","journal-title":"J Leukoc Biol"},{"key":"B85","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2014.08.005","article-title":"Dectin-2 is a direct receptor for mannose-capped lipoarabinomannan of mycobacteria","volume":"41","author":"Yonekawa","year":"2014","journal-title":"Immunity"},{"key":"B86","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrep.2016.05.008","article-title":"Induction of IL-10-producing regulatory B cells following Toxoplasma gondii infection is important to the cyst formation","volume":"7","author":"Jeong","year":"2016","journal-title":"Biochem Biophys Rep"},{"key":"B87","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2011.00305.x","article-title":"Mechanisms of Toxoplasma gondii persistence and latency","volume":"36","author":"Sullivan","year":"2012","journal-title":"FEMS Microbiol Rev"},{"key":"B88","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA118.003555","article-title":"PtpA, a secreted tyrosine phosphatase from Staphylococcus aureus, contributes to virulence and interacts with coronin-1A during infection","volume":"293","author":"Gannoun-Zaki","year":"2018","journal-title":"J Biol Chem"},{"key":"B89","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.677824","article-title":"PSGL-1 is a T cell intrinsic inhibitor that regulates effector and memory differentiation and responses during viral infection","volume":"12","author":"Tinoco","year":"2021","journal-title":"Front Immunol"},{"key":"B90","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines9040357","article-title":"Recombinant Toxoplasma gondii Ribosomal Protein P2 Modulates the Functions of Murine Macrophages In Vitro and Provides Immunity against Acute Toxoplasmosis In Vivo","volume":"9","author":"Yu","year":"2021","journal-title":"Vaccines"},{"key":"B91","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20041672","article-title":"Synergistic role of micronemal proteins in Toxoplasma gondii virulence","volume":"201","author":"C\u00e9r\u00e8de","year":"2005","journal-title":"J Exp Med"},{"key":"B92","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.9.4176-4182.1998","article-title":"Targeted disruption of the GRA2 locus in Toxoplasma gondii decreases acute virulence in mice","volume":"66","author":"Mercier","year":"1998","journal-title":"Infect Immun"},{"key":"B93","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1016\/B978-0-12-396481-6.00012-X","article-title":"Toxoplasma Secretory Proteins and Their Roles in Cell Invasion and Intracellular Survival","volume-title":"Toxoplasma Gondii","author":"Lebrun","year":"2014"},{"key":"B94","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002236","article-title":"Toxoplasma gondii rhoptry kinase ROP16 activates STAT3 and STAT6 resulting in cytokine inhibition and arginase-1-dependent growth control","volume":"7","author":"Butcher","year":"2011","journal-title":"PloS Pathog"},{"key":"B95","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2015.07.002","article-title":"Zymosan and PMA activate the immune responses of Mutz3-derived dendritic cells synergistically","volume":"167","author":"Song","year":"2015","journal-title":"Immunol Lett"},{"key":"B96","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.173.7.4479","article-title":"CpG DNA induces igG class switch DNA recombination by activating human B cells through an innate pathway that requires TLR9 and cooperates with IL-10","volume":"173","author":"He","year":"2004","journal-title":"J Immunol"},{"key":"B97","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2005.12.003","article-title":"Toll-like receptor recognition of Toxoplasma gondii","volume":"36","author":"Yarovinsky","year":"2006","journal-title":"Int J Parasitol"},{"key":"B98","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.pt.2006.10.001","article-title":"Toll road for Toxoplasma gondii: the mystery continues","volume":"23","author":"Khan","year":"2007","journal-title":"Trends Parasitol"},{"key":"B99","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxg108","article-title":"TLR2 as an essential molecule for protective immunity against Toxoplasma gondii infection","volume":"15","author":"Mun","year":"2003","journal-title":"Int Immunol"},{"key":"B100","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006739","article-title":"Voltage-dependent calcium channel signaling mediates GABAA receptor-induced migratory activation of dendritic cells infected by Toxoplasma gondii","volume":"13","author":"Kanatani","year":"2017","journal-title":"PloS Pathog"},{"key":"B101","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003051","article-title":"GABAergic signaling is linked to a hypermigratory phenotype in dendritic cells infected by toxoplasma gondii","volume":"8","author":"Fuks","year":"2012","journal-title":"PloS Pathog"},{"key":"B102","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830270141","article-title":"Constitutive macropinocytosis allows TAP-dependent major histocompatibility compex class I presentation of exogenous soluble antigen by bone marrow-derived dendritic cells","volume":"27","author":"Norbury","year":"1997","journal-title":"Eur J Immunol"},{"key":"B103","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2005.00517.x","article-title":"Dissemination of Toxoplasma gondii to immunoprivileged organs and role of Toll\/interleukin-1 receptor signalling for host resistance assessed by in vivo bioluminescence imaging","volume":"7","author":"Hitziger","year":"2005","journal-title":"Cell Microbiol"},{"key":"B104","doi-asserted-by":"publisher","first-page":"594","DOI":"10.1002\/cyto.a.22865","article-title":"A rapid method to verify single-cell deposition setup for cell sorters","volume":"89","author":"Rodrigues","year":"2016","journal-title":"Cytometry A"},{"key":"B105","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2639","article-title":"Smart-seq2 for sensitive full-length transcriptome profiling in single cells","volume":"10","author":"Picelli","year":"2013","journal-title":"Nat Methods"},{"key":"B106","doi-asserted-by":"publisher","first-page":"1888","DOI":"10.1016\/j.cell.2019.05.031","article-title":"Comprehensive integration of single-cell data","volume":"177","author":"Stuart","year":"2019","journal-title":"Cell"},{"key":"B107","doi-asserted-by":"publisher","first-page":"296","DOI":"10.1186\/s13059-019-1874-1","article-title":"Normalization and variance stabilization of single-cell RNA-seq data using regularized negative binomial regression","volume":"20","author":"Hafemeister","year":"2019","journal-title":"Genome Biol"},{"key":"B108","doi-asserted-by":"publisher","first-page":"223","DOI":"10.12688\/f1000research.22969.2","article-title":"clustifyr: an R package for automated single-cell RNA sequencing cluster classification","volume":"9","author":"Fu","year":"2020","journal-title":"F1000Res"},{"key":"B109","doi-asserted-by":"publisher","DOI":"10.1126\/science.aad0501","article-title":"Dissecting the multicellular ecosystem of metastatic melanoma by single-cell RNA-seq","volume":"352","author":"Tirosh","year":"2016","journal-title":"Science"},{"key":"B110","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-6-S1-S17","article-title":"An evaluation of GO annotation retrieval for BioCreAtIvE and GOA","author":"Camon","year":"2005","journal-title":"BMC Bioinf"},{"key":"B111","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol"}],"container-title":["Frontiers in Immunology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fimmu.2023.1224591\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,28]],"date-time":"2023-07-28T01:09:00Z","timestamp":1690506540000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fimmu.2023.1224591\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,27]]},"references-count":111,"alternative-id":["10.3389\/fimmu.2023.1224591"],"URL":"https:\/\/doi.org\/10.3389\/fimmu.2023.1224591","relation":{},"ISSN":["1664-3224"],"issn-type":[{"value":"1664-3224","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7,27]]},"article-number":"1224591"}}