{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T02:36:39Z","timestamp":1789094199659,"version":"build-2803163510"},"reference-count":68,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,1,30]],"date-time":"2023-01-30T00:00:00Z","timestamp":1675036800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Coxiella burnetii<\/jats:italic> is a zoonotic pathogen, infecting humans, livestock, pets, birds and ticks. Domestic ruminants such as cattle, sheep, and goats are the main reservoir and major cause of human infection. Infected ruminants are usually asymptomatic, while in humans infection can cause significant disease. Human and bovine macrophages differ in their permissiveness for <jats:italic>C. burnetii<\/jats:italic> strains from different host species and of various genotypes and their subsequent host cell response, but the underlying mechanism(s) at the cellular level are unknown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:italic>C. burnetii<\/jats:italic> infected primary human and bovine macrophages under normoxic and hypoxic conditions were analyzed for (i) bacterial replication by CFU counts and immunofluorescence; (ii) immune regulators by westernblot and qRT-PCR; cytokines by ELISA; and metabolites by gas chromatography-mass spectrometry (GC-MS).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Here, we confirmed that peripheral blood-derived human macrophages prevent <jats:italic>C. burnetii<\/jats:italic> replication under oxygen-limiting conditions. In contrast, oxygen content had no influence on <jats:italic>C. burnetii<\/jats:italic> replication in bovine peripheral blood-derived macrophages. In hypoxic infected bovine macrophages, STAT3 is activated, even though HIF1\u03b1 is stabilized, which otherwise prevents STAT3 activation in human macrophages. In addition, the TNF\u03b1 mRNA level is higher in hypoxic than normoxic human macrophages, which correlates with increased secretion of TNF\u03b1 and control of <jats:italic>C. burnetii<\/jats:italic> replication. In contrast, oxygen limitation does not impact TNF\u03b1 mRNA levels in <jats:italic>C. burnetii<\/jats:italic>-infected bovine macrophages and secretion of TNF\u03b1 is blocked. As TNF\u03b1 is also involved in the control of <jats:italic>C. burnetii<\/jats:italic> replication in bovine macrophages, this cytokine is important for cell autonomous control and its absence is partially responsible for the ability of <jats:italic>C. burnetii<\/jats:italic> to replicate in hypoxic bovine macrophages. Further unveiling the molecular basis of macrophage-mediated control of <jats:italic>C. burnetii<\/jats:italic> replication might be the first step towards the development of host directed intervention measures to mitigate the health burden of this zoonotic agent.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fimmu.2023.960927","type":"journal-article","created":{"date-parts":[[2023,1,30]],"date-time":"2023-01-30T09:06:14Z","timestamp":1675069574000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["Bovine blood derived macrophages are unable to control Coxiella burnetii replication under hypoxic conditions"],"prefix":"10.3389","volume":"14","author":[{"given":"Michael","family":"Mauermeir","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martha","family":"\u00d6lke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inaya","family":"Hayek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Schulze-Luehrmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katja","family":"Dettmer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter J.","family":"Oefner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Berens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Menge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anja","family":"L\u00fchrmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,1,30]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.12.4.518","article-title":"Q fever","volume":"12","author":"Maurin","year":"1999","journal-title":"Clin Microbiol Rev"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00045-16","article-title":"From Q fever to Coxiella burnetii infection: a paradigm change","volume":"30","author":"Eldin","year":"2017","journal-title":"Clin Microbiol Rev"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0155884","article-title":"Fatigue following acute Q-fever: A systematic literature review","volume":"11","author":"Morroy","year":"2016","journal-title":"PloS One"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1093\/qjmed\/91.2.105","article-title":"Post-infection fatigue syndrome following Q fever","volume":"91","author":"Ayres","year":"1998","journal-title":"QJM Monthly J Assoc Phys"},{"key":"B5","doi-asserted-by":"publisher","first-page":"110258","DOI":"10.1016\/j.jpsychores.2020.110258","article-title":"Long-term impact of a Q-fever outbreak: An evaluation of health symptoms, health-related quality of life, participation and health care satisfaction after ten years","volume":"139","author":"Bronner","year":"2020","journal-title":"J Psychosom Res"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.3201\/eid2107.130955","article-title":"Chronic Q fever diagnosis- consensus guideline versus expert opinion","volume":"21","author":"Kampschreur","year":"2015","journal-title":"Emerg Infect Dis"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cit419","article-title":"Evolution from acute Q fever to endocarditis is associated with underlying valvulopathy and age and can be prevented by prolonged antibiotic treatment","volume":"57","author":"Million","year":"2013","journal-title":"Clin Infect Dis"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2009.04511.x","article-title":"Q fever in dairy animals","volume":"1166","author":"Rodolakis","year":"2009","journal-title":"Ann New York Acad Sci"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2015.07.011","article-title":"Coxiella burnetii infections in sheep or goats: an opinionated review","volume":"181","author":"Van den Brom","year":"2015","journal-title":"Vet Microbiol"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.2807\/ese.18.08.20407-en","article-title":"Q fever in humans and farm animals in four European countries 1982 to 2010","volume":"18","author":"Georgiev","year":"2013","journal-title":"Euro Surveill"},{"key":"B11","doi-asserted-by":"publisher","first-page":"e232","DOI":"10.1017\/S0950268820001880","article-title":"Coxiella burnetii infection in humans: to what extent do cattle in infected areas free from small ruminants play a role","volume":"148","author":"Pouquet","year":"2020","journal-title":"Epidemiol Infect"},{"key":"B12","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.vetmic.2010.10.007","article-title":"Prevalence of Coxiella burnetii infection in domestic ruminants: a critical review","volume":"149","author":"Guatteo","year":"2011","journal-title":"Vet Microbiol"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268817002308","article-title":"Estimation of the frequency of Q fever in sheep, goat and cattle herds in France: results of a 3-year study of the seroprevalence of Q fever and excretion level of Coxiella burnetii in abortive episodes","volume":"145","author":"Gache","year":"2017","journal-title":"Epidemiol Infect"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2016-11863","article-title":"Herd-level prevalence of selected endemic infectious diseases of dairy cows in great Britain","volume":"100","author":"Velasova","year":"2017","journal-title":"J Dairy Sci"},{"key":"B15","doi-asserted-by":"publisher","first-page":"101489","DOI":"10.1016\/j.cimid.2020.101489","article-title":"Prevalence of Coxiella burnetii in central and Eastern European dairy herds","volume":"72","author":"Dobos","year":"2020","journal-title":"Comp Immunol Microbiol Infect Dis"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0210244","article-title":"Shedding and genetic diversity of Coxiella burnetii in polish dairy cattle","volume":"14","author":"Szymanska-Czerwinska","year":"2019","journal-title":"PloS One"},{"key":"B17","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1186\/s13567-017-0430-9","article-title":"Permissiveness of bovine epithelial cells from lung, intestine, placenta and udder for infection with Coxiella burnetii","volume":"48","author":"Sobotta","year":"2017","journal-title":"Vet Res"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2017.00543","article-title":"Interaction of Coxiella burnetii strains of different sources and genotypes with bovine and human monocyte-derived macrophages","volume":"7","author":"Sobotta","year":"2017","journal-title":"Front Cell Infect Microbiol"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01208-15","article-title":"Coxiella burnetii infects primary bovine macrophages and limits their host cell response","volume":"84","author":"Sobotta","year":"2016","journal-title":"Infect Immun"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.4.2345-2352.2001","article-title":"Interleukin-10 stimulates Coxiella burnetii replication in human monocytes through tumor necrosis factor down-modulation: role in microbicidal defect of Q fever","volume":"69","author":"Ghigo","year":"2001","journal-title":"Infect Immun"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-020-03684-8","article-title":"Mechanisms controlling bacterial infection in myeloid cells under hypoxic conditions","volume":"78","author":"Hayek","year":"2021","journal-title":"Cell Mol Life Sci"},{"key":"B22","doi-asserted-by":"publisher","first-page":"3502","DOI":"10.1016\/j.celrep.2019.02.103","article-title":"Limitation of TCA cycle intermediates represents an oxygen-independent nutritional antibacterial effector mechanism of macrophages","volume":"26","author":"Hayek","year":"2019","journal-title":"Cell Rep"},{"key":"B23","doi-asserted-by":"publisher","first-page":"393","DOI":"10.1016\/j.molcel.2008.04.009","article-title":"Oxygen sensing by metazoans: The central role of the HIF hydroxylase pathway","volume":"30","author":"Kaelin","year":"2008","journal-title":"Mol Cell"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1038\/nri2607","article-title":"Interdependence of hypoxic and innate immune responses","volume":"9","author":"Nizet","year":"2009","journal-title":"Nat Rev Immunol"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(94)90047-7","article-title":"A bioassay for the detection of tumor necrosis factor from eight different species: evaluation of neutralization rates of a monoclonal antibody against human TNF-alpha","volume":"171","author":"Pauli","year":"1994","journal-title":"J Immunol Methods"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2011.04.002","article-title":"Divergent cytokine responses of macrophages to Mycobacterium avium subsp. paratuberculosis strains of types II and III in a standardized in vitro model","volume":"152","author":"Borrmann","year":"2011","journal-title":"Vet Microbiol"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12494","article-title":"LAMP proteins account for the maturation delay during the establishment of the Coxiella burnetii-containing vacuole","volume":"18","author":"Schulze-Luehrmann","year":"2016","journal-title":"Cell Microbiol"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-010-4425-x","article-title":"Metabolite extraction from adherently growing mammalian cells for metabolomics studies: optimization of harvesting and extraction protocols","volume":"399","author":"Dettmer","year":"2011","journal-title":"Anal Bioanal Chem"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.12.5510","article-title":"Hypoxia-inducible factor 1 is a basic-helix-loop-helix-PAS heterodimer regulated by cellular O2 tension","volume":"92","author":"Wang","year":"1995","journal-title":"Proc Natl Acad Sci U.S.A."},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2012.125","article-title":"The updated biology of hypoxia-inducible factor","volume":"31","author":"Greer","year":"2012","journal-title":"EMBO J"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbcan.2005.05.001","article-title":"Negative and positive regulation of HIF-1: a complex network","volume":"1755","author":"Bardos","year":"2005","journal-title":"Biochim Biophys Acta"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1172\/JCI84431","article-title":"HIF1alpha and metabolic reprogramming in inflammation","volume":"126","author":"Corcoran","year":"2016","journal-title":"J Clin Invest"},{"key":"B33","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.cytogfr.2016.05.001","article-title":"STAT3 signaling in immunity","volume":"31","author":"Hillmer","year":"2016","journal-title":"Cytokine Growth Factor Rev"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1218599110","article-title":"Immune-responsive gene 1 protein links metabolism to immunity by catalyzing itaconic acid production","volume":"110","author":"Michelucci","year":"2013","journal-title":"Proc Natl Acad Sci U.S.A."},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1038\/nature25986","article-title":"Itaconate is an anti-inflammatory metabolite that activates Nrf2 via alkylation of KEAP1","volume":"556","author":"Mills","year":"2018","journal-title":"Nature"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005408","article-title":"IFNs modify the proteome of Legionella-containing vacuoles and restrict infection via IRG1-derived itaconic acid","volume":"12","author":"Naujoks","year":"2016","journal-title":"PloS Pathog"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.15252\/emmm.202215931","article-title":"Macrophages inhibit Coxiella burnetii by the ACOD1-itaconate pathway for containment of Q fever","author":"Kohl","year":"2022","journal-title":"EMBO Mol Med"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.651317","article-title":"The roles of 2-hydroxyglutarate","volume":"9","author":"Du","year":"2021","journal-title":"Front Cell Dev Biol"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200738067","article-title":"Coxiella burnetii, the agent of Q fever, stimulates an atypical M2 activation program in human macrophages","volume":"38","author":"Benoit","year":"2008","journal-title":"Eur J Immunol"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00411-16","article-title":"Murine alveolar macrophages are highly susceptible to replication of Coxiella burnetii phase II in vitro","volume":"84","author":"Fernandes","year":"2016","journal-title":"Infect Immun"},{"key":"B41","doi-asserted-by":"publisher","first-page":"998","DOI":"10.1128\/IAI.01536-15","article-title":"Primary role for toll-like receptor-driven tumor necrosis factor rather than cytosolic immune detection in restricting Coxiella burnetii phase II replication within mouse macrophages","volume":"84","author":"Bradley","year":"2016","journal-title":"Infect Immun"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-10-0064","article-title":"Comparison of mRNA and protein measures of cytokines following vaccination with human papillomavirus-16 L1 virus-like particles","volume":"19","author":"Shebl","year":"2010","journal-title":"Cancer Epidemiol Biomarkers Prev"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-75241-9_10","article-title":"Beginning to understand the role of the type IV secretion system effector proteins in Coxiella burnetii pathogenesis","volume":"413","author":"L\u00fchrmann","year":"2017","journal-title":"Curr Topics Microbiol Immunol"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(00)00325-7","article-title":"Q fever epidemiology and pathogenesis","volume":"2","author":"Norlander","year":"2000","journal-title":"Microbes Infect"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1128\/jb.154.2.591-597.1983","article-title":"Estimation of the cytoplasmic pH of Coxiella burnetii and effect of substrate oxidation on proton motive force","volume":"154","author":"Hackstadt","year":"1983","journal-title":"J Bacteriol"},{"key":"B46","doi-asserted-by":"publisher","first-page":"598","DOI":"10.1128\/jb.154.2.598-603.1983","article-title":"pH dependence of the Coxiella burnetii glutamate transport system","volume":"154","author":"Hackstadt","year":"1983","journal-title":"J Bacteriol"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.7.3815-3821.2000","article-title":"Coxiella burnetii exhibits morphological change and delays phagolysosomal fusion after internalization by J774A.1 cells","volume":"68","author":"Howe","year":"2000","journal-title":"Infect Immun"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00175-11","article-title":"Dot\/Icm type IVB secretion system requirements for Coxiella burnetii growth in human macrophages","volume":"2","author":"Beare","year":"2011","journal-title":"MBio"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002056","article-title":"The Coxiella burnetii Dot\/Icm system delivers a unique repertoire of type IV effectors into host cells and is required for intracellular replication","volume":"7","author":"Carey","year":"2011","journal-title":"PloS Pathog"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1016\/0966-842X(94)90651-3","article-title":"Survival of the Q fever agent Coxiella burnetii in the phagolysosome","volume":"2","author":"Baca","year":"1994","journal-title":"Trends Microbiol"},{"key":"B51","doi-asserted-by":"publisher","first-page":"66","DOI":"10.1016\/j.immuni.2013.11.020","article-title":"Transmigrating neutrophils shape the mucosal microenvironment through localized oxygen depletion to influence resolution of inflammation","volume":"40","author":"Campbell","year":"2014","journal-title":"Immunity"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1111\/febs.13270","article-title":"The impact of hypoxia on bacterial infection","volume":"282","author":"Schaffer","year":"2015","journal-title":"FEBS J"},{"key":"B53","article-title":"Q fever and seroprevalence of Coxiella burnetii in domestic ruminants","volume":"54","author":"Pexara","year":"2018","journal-title":"Vet Ital"},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.1051\/vetres:2005010","article-title":"Is Q fever an emerging or re-emerging zoonosis","volume":"36","author":"Arricau-Bouvery","year":"2005","journal-title":"Vet Res"},{"key":"B55","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1016\/j.rvsc.2011.07.017","article-title":"Serological screening for Coxiella burnetii infection and related reproductive performance in high producing dairy cows","volume":"93","author":"Lopez-Gatius","year":"2012","journal-title":"Res Vet Sci"},{"key":"B56","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1016\/S1074-7613(00)80005-9","article-title":"Enhanced Th1 activity and development of chronic enterocolitis in mice devoid of Stat3 in macrophages and neutrophils","volume":"10","author":"Takeda","year":"1999","journal-title":"Immunity"},{"key":"B57","doi-asserted-by":"publisher","DOI":"10.1002\/hep.29089","article-title":"The human longevity gene homolog INDY and interleukin-6 interact in hepatic lipid metabolism","volume":"66","author":"von Loeffelholz","year":"2017","journal-title":"Hepatology"},{"key":"B58","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2017.04.012","article-title":"STAT3 regulation of citrate synthase is essential during the initiation of lymphocyte cell growth","volume":"19","author":"MacPherson","year":"2017","journal-title":"Cell Rep"},{"key":"B59","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.00141","article-title":"A role for the Krebs cycle intermediate citrate in metabolic reprogramming in innate immunity and inflammation","volume":"9","author":"Williams","year":"2018","journal-title":"Front Immunol"},{"key":"B60","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2016.06.004","article-title":"Itaconate links inhibition of succinate dehydrogenase with macrophage metabolic remodeling and regulation of inflammation","volume":"24","author":"Lampropoulou","year":"2016","journal-title":"Cell Metab"},{"key":"B61","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2014.00491","article-title":"Macrophage cytokines: involvement in immunity and infectious diseases","volume":"5","author":"Arango Duque","year":"2014","journal-title":"Front Immunol"},{"key":"B62","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0800137105","article-title":"A trans-golgi network golgin is required for the regulated secretion of TNF in activated macrophages in vivo","volume":"105","author":"Lieu","year":"2008","journal-title":"Proc Natl Acad Sci U.S.A."},{"key":"B63","doi-asserted-by":"publisher","DOI":"10.1126\/science.1120225","article-title":"A role for the phagosome in cytokine secretion","volume":"310","author":"Murray","year":"2005","journal-title":"Science"},{"key":"B64","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M414420200","article-title":"Syntaxin 6 and Vti1b form a novel SNARE complex, which is up-regulated in activated macrophages to facilitate exocytosis of tumor necrosis factor-alpha","volume":"280","author":"Murray","year":"2005","journal-title":"J Biol Chem"},{"key":"B65","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(03)00006-X","article-title":"The t-SNARE syntaxin 4 is regulated during macrophage activation to function in membrane traffic and cytokine secretion","volume":"13","author":"Pagan","year":"2003","journal-title":"Curr Biol CB"},{"key":"B66","doi-asserted-by":"publisher","DOI":"10.1038\/385729a0","article-title":"A metalloproteinase disintegrin that releases tumour-necrosis factor-alpha from cells","volume":"385","author":"Black","year":"1997","journal-title":"Nature"},{"key":"B67","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1202500","article-title":"Synaptotagmin XI regulates phagocytosis and cytokine secretion in macrophages","volume":"190","author":"Arango Duque","year":"2013","journal-title":"J Immunol"},{"key":"B68","first-page":"1","article-title":"Diagnosis and management of Q fever\u2013united states 2013: recommendations from CDC and the Q fever working group","volume":"62","author":"Anderson","year":"2013","journal-title":"MMWR Recommendations Rep"}],"container-title":["Frontiers in Immunology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fimmu.2023.960927\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,30]],"date-time":"2023-01-30T09:06:18Z","timestamp":1675069578000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fimmu.2023.960927\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,30]]},"references-count":68,"alternative-id":["10.3389\/fimmu.2023.960927"],"URL":"https:\/\/doi.org\/10.3389\/fimmu.2023.960927","relation":{},"ISSN":["1664-3224"],"issn-type":[{"value":"1664-3224","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1,30]]},"article-number":"960927"}}