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Immunol."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>There is no useful method to discriminate between latent tuberculosis infection (LTBI) and active pulmonary tuberculosis (PTB). This study aimed to investigate the potential of cytokine profiles to discriminate between LTBI and active PTB using whole-blood stimulation with <jats:italic>Mycobacterium tuberculosis<\/jats:italic> (MTB) antigens, including latency-associated antigens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Patients with active PTB, household contacts of active PTB patients and community exposure subjects were recruited in Manila, the Philippines. Peripheral blood was collected from the participants and used for whole-blood stimulation (WBS) with either the early secretory antigenic target and the 10-kDa culture filtrate protein (ESAT-6\/CFP-10), Rv3879c or latency-associated MTB antigens, including mycobacterial DNA-binding protein 1 (MDP-1), \u03b1-crystallin (Acr) and heparin-binding hemagglutinin (HBHA). Multiple cytokine concentrations were analyzed using the Bio-Plex\u2122 multiplex cytokine assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 78 participants consisting of 15 active PTB patients, 48 household contacts and 15 community exposure subjects were eligible. The MDP-1-specific IFN-\u03b3 level in the active PTB group was significantly lower than that in the household contact group (p &amp;lt; 0.001) and the community exposure group (p &amp;lt; 0.001). The Acr-specific TNF-\u03b1 and IL-10 levels in the active PTB group were significantly higher than those in the household contact (TNF-\u03b1; p = 0.001, IL-10; p = 0.001) and community exposure (TNF-\u03b1; p &amp;lt; 0.001, IL-10; p = 0.01) groups. However, there was no significant difference in the ESAT-6\/CFP-10-specific IFN-\u03b3 levels among the groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The patterns of cytokine profiles induced by latency-associated MTB antigens using WBS have the potential to discriminate between LTBI and active PTB. In particular, combinations of IFN-\u03b3 and MDP-1, TNF-\u03b1 and Acr, and IL-10 and Acr are promising. This study provides the first demonstration of the utility of MDP-1-specific cytokine responses in WBS.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fimmu.2024.1330796","type":"journal-article","created":{"date-parts":[[2024,4,10]],"date-time":"2024-04-10T04:59:02Z","timestamp":1712725142000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":5,"title":["Evaluation of cytokine profiles related to Mycobacterium tuberculosis latent antigens using a whole-blood assay in the Philippines"],"prefix":"10.3389","volume":"15","author":[{"given":"Ikkoh","family":"Yasuda","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naomi Ruth D.","family":"Saludar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana 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