{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T01:59:30Z","timestamp":1767664770422,"version":"3.41.2"},"reference-count":48,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,3,28]],"date-time":"2024-03-28T00:00:00Z","timestamp":1711584000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>The ErbB-2.1(TOB1) signaling transducer protein is a tumor-suppressive protein that actively suppresses the malignant phenotype of gastric cancer cells. Yet, TOB1 negatively regulates the activation and growth of different immune cells. Understanding the expression and role of TOB1 in the gastric cancer immune environment is crucial to maximize its potential in targeted immunotherapy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This study employed multiplex immunofluorescence analysis to precisely delineate and quantify the expression of TOB1 in immune cells within gastric cancer tissue microarrays. Univariate and multivariate Cox analyses were performed to assess the influence of clinical-pathological parameters, immune cells, <jats:italic>TOB1<\/jats:italic>, and double-positive cells on the prognosis of gastric cancer patients. Subsequent experiments included co-culture assays of si-<jats:italic>TOB1<\/jats:italic>-transfected neutrophils with AGS or HGC-27 cells, along with EdU, invasion, migration assays, and bioinformatics analyses, aimed at elucidating the mechanisms through which <jats:italic>TOB1<\/jats:italic> in neutrophils impacts the prognosis of gastric cancer patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We remarkably revealed that TOB1 exhibits varying expression levels in both the nucleus (nTOB1) and cytoplasm (cTOB1) of diverse immune cell populations, including CD8<jats:sup>+<\/jats:sup> T cells, CD66b<jats:sup>+<\/jats:sup> neutrophils, FOXP3<jats:sup>+<\/jats:sup> Tregs, CD20<jats:sup>+<\/jats:sup> B cells, CD4<jats:sup>+<\/jats:sup> T cells, and CD68<jats:sup>+<\/jats:sup> macrophages within gastric cancer and paracancerous tissues. Significantly, TOB1 was notably concentrated in CD66b+ neutrophils. Survival analysis showed that a higher density of cTOB1\/nTOB1<jats:sup>+<\/jats:sup>CD66b<jats:sup>+<\/jats:sup> neutrophils was linked to a better prognosis. Subsequent experiments revealed that, following stimulation with the supernatant of tumor tissue culture, the levels of TOB1 protein and mRNA in neutrophils decreased, accompanied by enhanced apoptosis. HL-60 cells were successfully induced to neutrophil-like cells by DMSO. Neutrophils-like cells with attenuated TOB1 gene expression by si-TOB1 demonstrated heightened apoptosis, consequently fostering a malignant phenotype in AGS and HCG-27 cells upon co-cultivation. The subsequent analysis of the datasets from TCGA and TIMER2 revealed that patients with high levels of TOB1 combined neutrophils showed better immunotherapy response.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>This study significantly advances our comprehension of TOB1\u2019s role within the immune microenvironment of gastric cancer, offering promising therapeutic targets for immunotherapy in this context.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fimmu.2024.1369087","type":"journal-article","created":{"date-parts":[[2024,3,28]],"date-time":"2024-03-28T04:41:16Z","timestamp":1711600876000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["TOB1 modulates neutrophil phenotypes to influence gastric cancer progression and immunotherapy 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