{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T18:30:52Z","timestamp":1793644252313,"version":"4.5.1"},"reference-count":48,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T00:00:00Z","timestamp":1734393600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Progestin And AdipoQ Receptor Family Member VI (\n                      <jats:italic>PAQR6<\/jats:italic>\n                      ) plays a significant role in the non-genomic effects of rapid steroid responses and is abnormally expressed in various tumors. However, its biological function in kidney renal clear cell carcinoma (KIRC) and its potential as a therapeutic target remain underexplored.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      In this study,\n                      <jats:italic>PAQR6<\/jats:italic>\n                      was identified as a critical oncogene by WGCNA algorithm and differential gene expression analysis using TCGA - KIRC and GSE15641 data. The differences in\n                      <jats:italic>PAQR6<\/jats:italic>\n                      expression and its association with KIRC survival outcomes were investigated, and transcriptomic data were used to further elucidate\n                      <jats:italic>PAQR6<\/jats:italic>\n                      \u2019s biological functions. Moreover, XCELL and single - cell analysis assessed the correlation between\n                      <jats:italic>PAQR6<\/jats:italic>\n                      expression and immune infiltration. TIDE algorithm was used to assess how well various patient cohorts responded to immune checkpoint therapy. Finally, the role of\n                      <jats:italic>PAQR6<\/jats:italic>\n                      in the development of KIRC was verified through EdU, scratch assays, and Transwell assays.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Our findings suggest that elevated expression of\n                      <jats:italic>PAQR6<\/jats:italic>\n                      is linked to a poor prognosis for KIRC patients. Functional enrichment analysis demonstrated that\n                      <jats:italic>PAQR6<\/jats:italic>\n                      is primarily involved in angiogenesis and pluripotent stem cell differentiation, which are crucial in mediating the development of KIRC. Additionally, we established a ceRNA network that is directly related to overall prognosis, further supporting the role of\n                      <jats:italic>PAQR6<\/jats:italic>\n                      as a prognostic biomarker for KIRC.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Using both computational and experimental methods, this study leads the charge in discovering and verifying\n                      <jats:italic>PAQR6<\/jats:italic>\n                      as a prognostic biomarker and possible therapeutic target for KIRC. In the future, to determine its molecular mechanism in KIRC carcinogenesis, more\n                      <jats:italic>in vivo<\/jats:italic>\n                      research will be carried out.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fimmu.2024.1521629","type":"journal-article","created":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T01:25:45Z","timestamp":1734398745000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":7,"title":["PAQR6 as a prognostic biomarker and potential therapeutic target in kidney renal clear cell 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