{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:11:58Z","timestamp":1791216718908,"version":"4.1.0"},"reference-count":47,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,1,10]],"date-time":"2025-01-10T00:00:00Z","timestamp":1736467200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>Ulcerative colitis (UC) is a chronic inflammatory disease. Patients with UC typically exhibit disruption of the Treg\/Th17 immune axis, but its exact mechanism is still unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This study first analyzed RNA- seq data from public databases of humans and mice, and <jats:italic>in vitro<\/jats:italic> cytology experiments were conducted to induce or inhibit the expression of SIRT1. <jats:italic>In vivo<\/jats:italic>, UC mice were treated with moxibustion and SIRT1 inhibitor EX-527 to confirm the changes in the transcription factors identified through analysis of the datasets.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The results show that Treg\/Th17 axis disruption is an important feature of UC. Differential gene expression and immune infiltration analysis showed that upstream transcription factors, including Forkhead box P3 (FOXP3), were significantly disrupted. <jats:italic>In vitro<\/jats:italic> cytology experiments, the results indicate that SIRT1 is activated in LPS induced inflammation, subsequently perturbing the Treg\/Th17 immune balance axis. Finally, <jats:italic>in vivo<\/jats:italic> studies, the results have shown that administering EX-527 to inhibit SIRT1 leads to an increasing in FOXP3 expression and a decreasing in ROR\u03b3t expression in UC colon tissue. In addition, the results indicate that traditional Chinese moxibustion can down regulate the expression of SIRT1, directly affecting the balance of Th17\/Treg axis, and the combined use of EX-527 further improves the therapeutic effect of moxibustion.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our research shows that inhibition SIRT1 can regulate Treg and Th17 immune balance axis. This finding indicates a new important potential target for the treatment of UC.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fimmu.2024.1525469","type":"journal-article","created":{"date-parts":[[2025,1,10]],"date-time":"2025-01-10T05:12:18Z","timestamp":1736485938000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":14,"title":["Inhibition SIRT1 to regulate FOXP3 or ROR\u03b3t can restore the balance of Treg\/Th17 axis in ulcerative colitis and enhance the anti-inflammatory effect of 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