{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:09:07Z","timestamp":1788307747396,"version":"build-2803163510"},"reference-count":31,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,1,14]],"date-time":"2025-01-14T00:00:00Z","timestamp":1736812800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:p>Heat-stress-induced oxidative and inflammatory responses were important factors contributing to chicken intestinal damage. The purpose of this study was based on the antioxidant and anti-inflammatory activities of Physalis Calyx seu Fructus (Jin Deng Long, JDL) to investigate its efficacy and mechanism in relieving chicken heat stress damage. Primary chicken embryo duodenum cells and 90 30-day-old specific-pathogen-free chicken were randomly divided into control and JDL groups to establish heat stress models <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic>. The mitigating effect was assessed through the oxidation-related enzymes and key genes, histopathology, and inflammatory factors. The results demonstrated that 100 \u00b5g\/mL JDL extract could effectively alleviate heat stress damage to chicken embryo duodenum cells at 42\u00b0C. A strong antioxidant capacity of 100 \u00b5g\/mL JDL extract was shown in the downregulation of LDH (at 5 h, <jats:italic>P<\/jats:italic> &amp;lt; 0.01) and MDA (at 5 h, <jats:italic>P<\/jats:italic> &amp;lt; 0.05), in the upregulation of SOD (at 5 and 10 h, <jats:italic>P<\/jats:italic> &amp;lt; 0.01), CAT (at 5 h, <jats:italic>P<\/jats:italic> &amp;lt; 0.01), and GSH-PX and T-AOC (at 0 h, <jats:italic>P<\/jats:italic> &amp;lt; 0.01) as well as in the high transcription level of <jats:italic>NQO1<\/jats:italic> (at 5 and 10 h, <jats:italic>P<\/jats:italic> &amp;lt; 0.05) and <jats:italic>HO-1<\/jats:italic> (at 5 and 10 h, <jats:italic>P<\/jats:italic> &amp;lt; 0.01). Supplements with 1 and 3 g\/kg b.wt, respectively, in the drinking water both suppressed the rise of body temperature and had light pathological lesions of chicken duodenal tissues caused by heat stress at 40 \u00b1 1\u00b0C. Accordingly, the chicken of JDL extract groups showed a lower inflammatory response as manifested by a lower level of IL-10 and higher levels of IL-6 and TNF-\u03b1 and a strong antioxidant capacity characterized by lower level of MDA and higher levels of SOD and GSH-PX in the serum as well as also showed a higher transcription level of <jats:italic>Nrf2<\/jats:italic>, <jats:italic>NQO1<\/jats:italic>, and <jats:italic>HO-1<\/jats:italic> in the duodenal tissues. In conclusion, JDL extract relieved chicken intestinal damage to heat via improving the antioxidant ability and reducing the inflammatory response.<\/jats:p>","DOI":"10.3389\/fimmu.2024.1536045","type":"journal-article","created":{"date-parts":[[2025,1,14]],"date-time":"2025-01-14T05:10:55Z","timestamp":1736831455000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Physalis Calyx seu Fructus relieves chicken intestinal damage to heat via improving the antioxidant ability"],"prefix":"10.3389","volume":"15","author":[{"given":"Bin","family":"Yin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changning","family":"Juan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongling","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shifa","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiting","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yueyue","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shikai","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zunxiang","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunpeng","family":"Yi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zengcheng","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongli","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuqian","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,1,14]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1007\/s00484-020-02023-7","article-title":"Heat stress and poultry production: impact and amelioration","volume":"65","author":"Vandana","year":"2021","journal-title":"Int J Biometeorol"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1111\/jpn.13916","article-title":"Strategies to combat heat stress in poultry production-A review","volume":"108","author":"Mangan","year":"2024","journal-title":"J Anim Physiol Anim Nutr (Berl)"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.3390\/nu12030734","article-title":"Beyond heat stress: intestinal integrity disruption and mechanism-based intervention strategies","volume":"12","author":"Lian","year":"2020","journal-title":"Nutrients"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1820130116","article-title":"Heat stress directly impairs gut integrity and recruits distinct immune cell populations into the bovine intestine","volume":"116","author":"Koch","year":"2019","journal-title":"Proc Natl Acad Sci U S A"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1016\/j.domaniend.2022.106745","article-title":"Quantifying heat stress; the roles on metabolic status and intestinal integrity in poultry, a review","volume":"81","author":"Hosseindoust","year":"2022","journal-title":"Domest Anim Endocrinol"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00787.2001","article-title":"Selected contribution: Hyperthermia-induced intestinal permeability and the role of oxidative and nitrosative stress","author":"Lambert","year":"1985","journal-title":"J Appl Physiol"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2013-03455","article-title":"Effect of a probiotic mixture on intestinal microflora, morphology, and barrier integrity of broilers subjected to heat stress","volume":"93","author":"Song","year":"2014","journal-title":"Poult Sci"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2017.08.022","article-title":"Physalis alkekengi L. var. franchetii (Mast.) 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