{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T18:45:39Z","timestamp":1787510739029,"version":"build-2736575974"},"reference-count":119,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T00:00:00Z","timestamp":1742169600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:p>\n                    Catfish production is the primary aquaculture sector in the United States, and the key cultured species is channel catfish (\n                    <jats:italic>Ictalurus punctatus<\/jats:italic>\n                    ). The major causes of production losses are pathogenic diseases, and the spleen, an important site of adaptive immunity, is implicated in these diseases. To examine the channel catfish immune system, single-nuclei transcriptomes of sorted and captured IgM\n                    <jats:sup>+<\/jats:sup>\n                    cells were produced from adult channel catfish. Three channel catfish (~1 kg) were euthanized, the spleen dissected, and the tissue dissociated. The lymphocytes were isolated using a Ficoll gradient and IgM\n                    <jats:sup>+<\/jats:sup>\n                    cells were then sorted with flow cytometry. The IgM\n                    <jats:sup>+<\/jats:sup>\n                    cells were lysed and single-nuclei libraries generated using a Chromium Next GEM Single Cell 3\u2019 GEM Kit and the Chromium X Instrument (10x Genomics) and sequenced with the Illumina NovaSeq X Plus sequencer. The reads were aligned to the\n                    <jats:italic>I. punctatus<\/jats:italic>\n                    reference assembly (Coco_2.0) using Cell Ranger, and normalization, cluster analysis, and differential gene expression analysis were carried out with Seurat. Across the three samples, approximately 753.5 million reads were generated for 18,686 cells. After filtering, 10,637 cells remained for the cluster analysis. The cluster analysis identified 16 clusters which were classified as B cells (10,276), natural killer-like (NK-like) cells (178), T cells or natural killer cells (45), hematopoietic stem and progenitor cells (HSPC)\/megakaryocytes (MK) (66), myeloid\/epithelial cells (40), and plasma cells (32). The B cell clusters were further defined as different populations of mature B cells, cycling B cells, and plasma cells. The plasma cells highly expressed\n                    <jats:italic>ighm<\/jats:italic>\n                    and we demonstrated that the secreted form of the transcript was largely being expressed by these cells. This atlas provides insight into the gene expression of IgM\n                    <jats:sup>+<\/jats:sup>\n                    immune cells in channel catfish. The atlas is publicly available and could be used garner more important information regarding the gene expression of splenic immune cells.\n                  <\/jats:p>","DOI":"10.3389\/fimmu.2025.1547193","type":"journal-article","created":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T03:19:29Z","timestamp":1742181569000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Single-nuclei transcriptome analysis of IgM+ cells isolated from channel catfish (Ictalurus punctatus) spleen"],"prefix":"10.3389","volume":"16","author":[{"given":"Johanna E.","family":"Aldersey","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason W.","family":"Abernathy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin H.","family":"Beck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miles D.","family":"Lange","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,3,17]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1185383","article-title":"Food security: The challenge of feeding 9 billion people","volume":"327","author":"Godfray","year":"2010","journal-title":"Science"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1038\/nature10452","article-title":"Solutions for a cultivated planet","volume":"478","author":"Foley","year":"2011","journal-title":"Nature"},{"key":"B3","volume-title":"Quick stats: livestock and animals","year":"2023"},{"key":"B4","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1002\/aah.10207","article-title":"Flavobacterium covae is the predominant species of columnaris-causing bacteria impacting the Channel Catfish industry in the southeastern United States","volume":"36","author":"LaFrentz","year":"2024","journal-title":"J Aquat Anim Health"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2022.739206","article-title":"Epidemiology and economic impact of disease-related losses on commercial catfish farms: A seven-year case study from Alabama, USA","volume":"566","author":"Abdelrahman","year":"2023","journal-title":"Aquaculture"},{"key":"B6","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1016\/j.dci.2005.06.016","article-title":"Channel catfish immunoglobulins: Repertoire and expression","volume":"30","author":"Bengt\u00e9n","year":"2006","journal-title":"Dev Comp Immunol"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1000631","article-title":"Identification of two IgD+ B cell populations in channel catfish, Ictalurus punctatus","volume":"185","author":"Edholm","year":"2010","journal-title":"J Immunol"},{"key":"B8","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1016\/0165-2427(86)90104-2","article-title":"Structural diversity of channel catfish immunoglobulins","volume":"12","author":"Lobb","year":"1986","journal-title":"Veterinary Immunol Immunopathol"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1998.tb01259.x","article-title":"Evolutionary variation of immunoglobulin mu heavy chain RNA processing pathways: origins, effects, and implications","volume":"166","author":"Ross","year":"1998","journal-title":"Immunol Rev"},{"key":"B10","doi-asserted-by":"publisher","first-page":"898","DOI":"10.1016\/j.dci.2011.01.009","article-title":"Dissecting teleost B cell differentiation using transcription factors","volume":"35","author":"Zwollo","year":"2011","journal-title":"Dev Comp Immunol"},{"key":"B11","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1007\/s00251-020-01196-0","article-title":"Anatomy of teleost fish immune structures and organs","volume":"73","author":"Bj\u00f8rgen","year":"2021","journal-title":"Immunogenetics"},{"key":"B12","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.fsi.2023.109280","article-title":"The fish spleen","volume":"144","author":"Zapata","year":"2024","journal-title":"Fish Shellfish Immunol"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2024.1456220","article-title":"Both sides now: evolutionary traits of antigens and B cells in tolerance and activation","volume":"15","author":"Hong","year":"2024","journal-title":"Front Immunol"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2011.03.005","article-title":"Plasmablasts and plasma cells: Reconsidering teleost immune system organization","volume":"35","author":"Ye","year":"2011","journal-title":"Dev Comp Immunol"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1093\/femsle\/fnw080","article-title":"Detection and quantification of virulent Aeromonas hydrophila in channel catfish tissues following waterborne challenge","volume":"363","author":"Zhang","year":"2016","journal-title":"FEMS Microbiol Letters"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2006.00310.x","article-title":"Development of bioluminescent Edwardsiella ictaluri for noninvasive disease monitoring","volume":"260","author":"Karsi","year":"2006","journal-title":"FEMS Microbiol Letters"},{"key":"B17","doi-asserted-by":"publisher","first-page":"109866","DOI":"10.1016\/j.fsi.2024.109866","article-title":"Single-cell transcriptome landscape of the kidney reveals potential innate immune regulation mechanisms in hybrid yellow catfish after Aeromonas hydrophila infection","volume":"153","author":"Guo","year":"2024","journal-title":"Fish Shellfish Immunol"},{"key":"B18","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1038\/s41368-021-00146-0","article-title":"From bulk, single-cell to spatial RNA sequencing","volume":"13","author":"Li","year":"2021","journal-title":"Int J Oral Science"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0309397","article-title":"Single-nuclei transcriptome analysis of channel catfish spleen provides insight into the immunome of an aquaculture-relevant species","volume":"19","author":"Aldersey","year":"2024","journal-title":"PLoS One"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202201505RRRR","article-title":"Single-cell transcriptome profiling reveals diverse immune cell populations and their responses to viral infection in the spleen of zebrafish","volume":"37","author":"Hu","year":"2023","journal-title":"FASEB J"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2024.109358","article-title":"Cell atlas of the Atlantic salmon spleen reveals immune cell heterogeneity and cell-specific responses to bacterial infection","volume":"145","author":"Sun","year":"2024","journal-title":"Fish Shellfish Immunol"},{"key":"B22","doi-asserted-by":"publisher","first-page":"100086","DOI":"10.1016\/j.fsirep.2023.100086","article-title":"A recombinant 9E1 monoclonal antibody binds membrane and soluble channel catfish immunoglobulin M","volume":"4","author":"Lange","year":"2023","journal-title":"Fish Shellfish Immunol Rep"},{"key":"B23","doi-asserted-by":"publisher","first-page":"100141","DOI":"10.1016\/j.xinn.2021.100141","article-title":"clusterProfiler 4.0: A universal enrichment tool for interpreting omics data","volume":"2","author":"Wu","year":"2021","journal-title":"Innovation (Camb)"},{"key":"B24","article-title":"UniProt: the Universal Protein Knowledgebase in 2023","volume":"51","year":"2022","journal-title":"Nucleic Acids Res"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac993","article-title":"InterPro in 2022","volume":"51","author":"Paysan-Lafosse","year":"2023","journal-title":"Nucleic Acids Res"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-021-01156-3","article-title":"SignalP 6.0 predicts all five types of signal peptides using protein language models","volume":"40","author":"Teufel","year":"2022","journal-title":"Nat Biotechnol"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae237","article-title":"DeepLoc 2.1: multi-label membrane protein type prediction using protein language models","volume":"52","author":"\u00d8dum","year":"2024","journal-title":"Nucleic Acids Res"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.536326","article-title":"The regulatory function of CCR9+ dendritic cells in inflammation and autoimmunity","volume":"11","author":"Pathak","year":"2020","journal-title":"Front Immunol"},{"key":"B29","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1016\/j.msard.2019.06.016","article-title":"Fingolimod reduces CXCR4-mediated B cell migration and induces regulatory B cells-mediated anti-inflammatory immune repertoire","volume":"34","author":"Blumenfeld-Kan","year":"2019","journal-title":"Multiple Sclerosis Related Disord"},{"key":"B30","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1016\/j.jaci.2013.12.1083","article-title":"The BLNK adaptor protein has a nonredundant role in human B-cell differentiation","volume":"134","author":"Lagresle-Peyrou","year":"2014","journal-title":"J Allergy Clin Immunol"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2009.12.006","article-title":"Early growth response transcription factors and the modulation of immune response: Implications towards autoimmunity","volume":"9","author":"G\u00f3mez-Mart\u00edn","year":"2010","journal-title":"Autoimmun Rev"},{"key":"B32","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/j.bbagrm.2009.09.001","article-title":"Nuclear functions of the HMG proteins","volume":"1799","author":"Reeves","year":"2010","journal-title":"Biochim Biophys Acta (BBA) - Gene Regul Mechanisms"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2020.108517","article-title":"Chromatin Protein PC4 Orchestrates B Cell Differentiation by Collaborating with IKAROS and IRF4","volume":"33","author":"Ochiai","year":"2020","journal-title":"Cell Rep"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0046093","article-title":"Pno1 tissue-specific expression and its functions related to the immune responses and proteasome activities","volume":"7","author":"Wang","year":"2012","journal-title":"PLoS One"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2017.67","article-title":"Cytoskeletal control of B cell responses to antigens","volume":"17","author":"Tolar","year":"2017","journal-title":"Nat Rev Immunol"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1126\/science.aax9198","article-title":"A genome-wide transcriptomic analysis of protein-coding genes in human blood cells","volume":"366","author":"Uhlen","year":"2019","journal-title":"Science"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2012.08.001","article-title":"The transcription factors Egr2 and Egr3 are essential for the control of inflammation and antigen-induced proliferation of B and T cells","volume":"37","author":"Li","year":"2012","journal-title":"Immunity"},{"key":"B38","doi-asserted-by":"publisher","first-page":"104658","DOI":"10.1016\/j.jbc.2023.104658","article-title":"eIF3d: A driver of noncanonical cap\u2013dependent translation of specific mRNAs and a trigger of biological\/pathological processes","volume":"299","author":"Ma","year":"2023","journal-title":"J Biol Chem"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1403088","article-title":"Metformin suppresses systemic autoimmunity in Roquinsan\/san Mice through inhibiting B cell differentiation into plasma cells via regulation of AMPK\/mTOR\/STAT3","volume":"198","author":"Lee","year":"2017","journal-title":"J Immunol"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-019-0492-y","article-title":"Disruption of the Myc-PDE4B regulatory circuitry impairs B-cell lymphoma survival","volume":"33","author":"Nam","year":"2019","journal-title":"Leukemia"},{"key":"B41","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1038\/sj.cr.7290065","article-title":"Costimulation of resting B lymphocytes alters the IL-4-activated IRS2 signaling pathway in a STAT6 independent manner: implications for cell survival and proliferation","volume":"11","author":"Zamorano","year":"2001","journal-title":"Cell Res"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2015.05.013","article-title":"Phosphatidylinositol (3,4) bisphosphate-specific phosphatases and effector proteins: A distinct branch of PI3K signaling","volume":"27","author":"Li","year":"2015","journal-title":"Cell Signalling"},{"key":"B43","doi-asserted-by":"publisher","first-page":"595","DOI":"10.4049\/jimmunol.2000088","article-title":"BCALM (AC099524.1) is a Human B lymphocyte\u2013specific long noncoding RNA that modulates B cell receptor\u2013mediated calcium signaling","volume":"205","author":"Pyfrom","year":"2020","journal-title":"J Immunol"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1111\/imr.v307.1","article-title":"NR4A nuclear receptors in T and B lymphocytes: Gatekeepers of immune tolerance","volume":"307","author":"Hiwa","year":"2022","journal-title":"Immunol Rev"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-020-0765-7","article-title":"NR4A nuclear receptors restrain B cell responses to antigen when second signals are absent or limiting","volume":"21","author":"Tan","year":"2020","journal-title":"Nat Immunol"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1502163","article-title":"CD83 modulates B cell activation and germinal center responses","volume":"196","author":"Krzyzak","year":"2016","journal-title":"J Immunol"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1111\/pim.12979","article-title":"CD193 (CCR3) expression by B cells correlates with reduced IgE production in paediatric schistosomiasis","volume":"45","author":"Onkanga","year":"2023","journal-title":"Parasite Immunol"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1038\/nrd.2016.253","article-title":"From basic apoptosis discoveries to advanced selective BCL-2 family inhibitors","volume":"16","author":"Ashkenazi","year":"2017","journal-title":"Nat Rev Drug Discov"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2012.119","article-title":"Histone H3K27me3 demethylases KDM6A and KDM6B modulate definitive endoderm differentiation from human ESCs by regulating WNT signaling pathway","volume":"23","author":"Jiang","year":"2013","journal-title":"Cell Res"},{"key":"B50","doi-asserted-by":"publisher","first-page":"188","DOI":"10.1016\/j.immuni.2011.01.014","article-title":"Nuclear export of the NF-\u03baB inhibitor I\u03baB\u03b1 is required for proper B cell and secondary lymphoid tissue formation","volume":"34","author":"Wuerzberger-Davis","year":"2011","journal-title":"Immunity"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200838254","article-title":"I\u03baB\u03b1 is required for marginal zone B cell lineage development","volume":"38","author":"Ellinghaus","year":"2008","journal-title":"Eur J Immunol"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0097953","article-title":"KLF2\u2013 a negative regulator of pre-B cell clonal expansion and B cell activation","volume":"9","author":"Winkelmann","year":"2014","journal-title":"PLoS One"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2021.757169","article-title":"RAB11FIP1: An indicator for tumor immune microenvironment and prognosis of lung adenocarcinoma from a comprehensive analysis of bioinformatics","volume":"12","author":"Zhang","year":"2021","journal-title":"Front Genet"},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20221020","article-title":"Human PIK3R1 mutations disrupt lymphocyte differentiation to cause activated PI3K\u03b4 syndrome 2","volume":"220","author":"Nguyen","year":"2023","journal-title":"J Exp Med"},{"key":"B55","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20212333","article-title":"Bhlhe40: Gatekeeper of the GC","volume":"219","author":"Nutt","year":"2021","journal-title":"J Exp Med"},{"key":"B56","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2008.05.008","article-title":"B cell receptor accessory molecules in the channel catfish, Ictalurus punctatus","volume":"32","author":"Sahoo","year":"2008","journal-title":"Dev Comp Immunol"},{"key":"B57","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1016\/S0065-230X(02)86006-7","article-title":"The life and death of a B cell","volume":"86","author":"Defrance","year":"2002","journal-title":"Adv Cancer Res"},{"key":"B58","doi-asserted-by":"publisher","DOI":"10.1002\/art.39193","article-title":"Syndecan 4 regulation of the development of autoimmune arthritis in mice by modulating B cell migration and germinal center formation","volume":"67","author":"Endo","year":"2015","journal-title":"Arthritis Rheumatol"},{"key":"B59","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1385\/IR:24:2:097","article-title":"Signaling by CD40 and its mimics in B cell activation","volume":"24","author":"Bishop","year":"2001","journal-title":"Immunologic Res"},{"key":"B60","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200737972","article-title":"Dynamic regulation of c-Myc proto-oncogene expression during lymphocyte development revealed by a GFP-c-Myc knock-in mouse","volume":"38","author":"Huang","year":"2008","journal-title":"Eur J Immunol"},{"key":"B61","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.00549","article-title":"ASCT2 (SLC1A5)-deficient mice have normal B-cell development, proliferation, and antibody production","volume":"8","author":"Masle-Farquhar","year":"2017","journal-title":"Front Immunol"},{"key":"B62","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2009.00827.x","article-title":"SLAP, a regulator of immunoreceptor ubiquitination, signaling, and trafficking","volume":"232","author":"Dragone","year":"2009","journal-title":"Immunol Rev"},{"key":"B63","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.913275","article-title":"RelB contributes to the survival, migration and lymphomagenesis of B cells with constitutively active CD40 signaling","volume":"13","author":"Kuhn","year":"2022","journal-title":"Front Immunol"},{"key":"B64","doi-asserted-by":"publisher","first-page":"92","DOI":"10.1006\/cimm.1995.1191","article-title":"Membrane immunoglobulin receptor cross-linking induces fosB mRNA expression in mature B lymphocytes: Assembly of distinct fosB-containing nucleoprotein complexes during B cell stimulation","volume":"165","author":"Dickinson","year":"1995","journal-title":"Cell Immunol"},{"key":"B65","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1006\/smim.1998.0122","article-title":"Positive and negative roles of the tyrosine kinase Lyn in B cell function","volume":"10","author":"DeFranco","year":"1998","journal-title":"Semin Immunol"},{"key":"B66","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2016.144964","article-title":"Inhibition of demethylase KDM6B sensitizes diffuse large B-cell lymphoma to chemotherapeutic drugs","volume":"102","author":"Rohit","year":"2017","journal-title":"Haematologica"},{"key":"B67","doi-asserted-by":"publisher","first-page":"183","DOI":"10.1111\/j.1600-065X.2008.00652.x","article-title":"Regulation of B-cell entry into the cell cycle","volume":"224","author":"Richards","year":"2008","journal-title":"Immunol Rev"},{"key":"B68","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2906-4","article-title":"HSP40 proteins use class-specific regulation to drive HSP70 functional diversity","volume":"587","author":"Faust","year":"2020","journal-title":"Nature"},{"key":"B69","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2017.20","article-title":"The HSP90 chaperone machinery","volume":"18","author":"Schopf","year":"2017","journal-title":"Nat Rev Mol Cell Biol"},{"key":"B70","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-031-14740-1_4","article-title":"Specification of Hsp70 function by Hsp40 co-chaperones","volume-title":"The Networking of Chaperones by Co-Chaperones","author":"Cyr","year":"2023"},{"key":"B71","doi-asserted-by":"publisher","first-page":"166634","DOI":"10.1016\/j.jmb.2020.08.019","article-title":"HSP70, a novel regulatory molecule in B cell-mediated suppression of autoimmune diseases","volume":"433","author":"Wang","year":"2021","journal-title":"J Mol Biol"},{"key":"B72","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1007\/s00428-013-1433-1","article-title":"Expression of peroxiredoxins I and IV in multiple myeloma: association with immunoglobulin accumulation","volume":"463","author":"Demasi","year":"2013","journal-title":"Virchows Archiv"},{"key":"B73","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.8.5337","article-title":"ERK signaling is a molecular switch integrating opposing inputs from B cell receptor and T cell cytokines to control TLR4-driven plasma cell differentiation","volume":"177","author":"Rui","year":"2006","journal-title":"J Immunol"},{"key":"B74","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1007\/978-981-15-3532-1_2","article-title":"B Cell Receptor Signaling","volume-title":"B Cells in Immunity and Tolerance","author":"Tanaka","year":"2020"},{"key":"B75","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2016.40","article-title":"Ubiquitin signaling in immune responses","volume":"26","author":"Hu","year":"2016","journal-title":"Cell Res"},{"key":"B76","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-020-0591-3","article-title":"Generalizing RNA velocity to transient cell states through dynamical modeling","volume":"38","author":"Bergen","year":"2020","journal-title":"Nat Biotechnol"},{"key":"B77","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.4.2505","article-title":"Identification and Characterization of a FcR Homolog in an Ectothermic Vertebrate, the Channel Catfish (Ictalurus punctatus)12","volume":"177","author":"Stafford","year":"2006","journal-title":"J Immunol"},{"key":"B78","doi-asserted-by":"publisher","first-page":"699","DOI":"10.1016\/S0145-305X(03)00042-9","article-title":"Channel catfish NK-like cells are armed with IgM via a putative Fc\u03bcR","volume":"27","author":"Shen","year":"2003","journal-title":"Dev Comp Immunol"},{"key":"B79","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2005.09.023","article-title":"NK-lysin of channel catfish: Gene triplication, sequence variation, and expression analysis","volume":"43","author":"Wang","year":"2006","journal-title":"Mol Immunol"},{"key":"B80","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.181.5.3177","article-title":"KLRE\/I1 and KLRE\/I2: A novel pair of heterodimeric receptors that inversely regulate NK cell cytotoxicity","volume":"181","author":"Saether","year":"2008","journal-title":"J Immunol"},{"key":"B81","doi-asserted-by":"publisher","DOI":"10.1016\/S0145-305X(03)00119-8","article-title":"Identification and characterization of clonal NK-like cells from channel catfish (Ictalurus punctatus)","volume":"28","author":"Shen","year":"2004","journal-title":"Dev Comp Immunol"},{"key":"B82","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.792334","article-title":"Filamin A is required for NK cell cytotoxicity at the expense of cytokine production via synaptic filamentous actin modulation","volume":"12","author":"Kim","year":"2022","journal-title":"Front Immunol"},{"key":"B83","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1038\/s41590-017-0001-2","article-title":"Single-cell analysis reveals the continuum of human lympho-myeloid progenitor cells","volume":"19","author":"Karamitros","year":"2018","journal-title":"Nat Immunol"},{"key":"B84","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2022.1009160","article-title":"Markers for human haematopoietic stem cells: The disconnect between an identification marker and its function","volume":"13","author":"Rix","year":"2022","journal-title":"Front Physiol"},{"key":"B85","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V96.13.4169","article-title":"The \u03b1IIb\u03b23 integrin and GPIb-V-IX complex identify distinct stages in the maturation of CD34+cord blood cells to megakaryocytes","volume":"96","author":"Lepage","year":"2000","journal-title":"Blood"},{"key":"B86","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2017.12.017","article-title":"MEIS1 regulates hemogenic endothelial generation, megakaryopoiesis, and thrombopoiesis in human pluripotent stem cells by targeting TAL1 and FLI1","volume":"10","author":"Wang","year":"2018","journal-title":"Stem Cell Rep"},{"key":"B87","doi-asserted-by":"publisher","first-page":"535","DOI":"10.1016\/j.stem.2020.11.006","article-title":"Decoding human megakaryocyte development","volume":"28","author":"Wang","year":"2021","journal-title":"Cell Stem Cell"},{"key":"B88","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-03-209932","article-title":"MAL\/SRF complex is involved in platelet formation and megakaryocyte migration by regulating MYL9 (MLC2) and MMP9","volume":"114","author":"Gilles","year":"2009","journal-title":"Blood"},{"key":"B89","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00056.2012","article-title":"Expression of plasma membrane receptor genes during megakaryocyte development","volume":"45","author":"Sun","year":"2013","journal-title":"Physiol Genomics"},{"key":"B90","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1512076112","article-title":"Prospective isolation of human erythroid lineage-committed progenitors","volume":"112","author":"Mori","year":"2015","journal-title":"Proc Natl Acad Sci"},{"key":"B91","doi-asserted-by":"publisher","first-page":"395","DOI":"10.1016\/S0301-472X(98)00077-0","article-title":"Characterization of defective megakaryocytic development in patients with myelodysplastic syndromes","volume":"27","author":"Hofmann","year":"1999","journal-title":"Exp Hematol"},{"key":"B92","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0043300","article-title":"Itga2b regulation at the onset of definitive hematopoiesis and commitment to differentiation","volume":"7","author":"Dumon","year":"2012","journal-title":"PloS One"},{"key":"B93","doi-asserted-by":"publisher","DOI":"10.1002\/stem.1434","article-title":"Expression levels of endoglin distinctively identify hematopoietic and endothelial progeny at different stages of yolk sac hematopoiesis","volume":"31","author":"Borges","year":"2013","journal-title":"Stem Cells"},{"key":"B94","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.119.312129","article-title":"New insights into the differentiation of megakaryocytes from hematopoietic progenitors","volume":"39","author":"Noetzli","year":"2019","journal-title":"Arteriosclerosis Thrombosis Vasc Biol"},{"key":"B95","doi-asserted-by":"publisher","DOI":"10.1111\/sji.13170","article-title":"Advances in CD247","volume":"96","author":"Dexiu","year":"2022","journal-title":"Scandinavian J Immunol"},{"key":"B96","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2004.03.008","article-title":"Cytotoxic activity of the lymphocyte toxin granzyme B","volume":"6","author":"Wowk","year":"2004","journal-title":"Microbes Infection"},{"key":"B97","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0306622101","article-title":"Copper-induced ferroportin-1 expression in J774 macrophages is associated with increased iron efflux","volume":"101","author":"Chung","year":"2004","journal-title":"Proc Natl Acad Sci"},{"key":"B98","doi-asserted-by":"publisher","first-page":"148027","DOI":"10.1016\/j.gene.2023.148027","article-title":"The kruppel-like factor (KLF) family, diseases, and physiological events","volume":"895","author":"Yuce","year":"2024","journal-title":"Gene"},{"key":"B99","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2023.1222265","article-title":"JunB: a paradigm for Jun family in immune response and cancer","volume":"13","author":"Ren","year":"2023","journal-title":"Front Cell Infect Microbiol"},{"key":"B100","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.179.11.7215","article-title":"Cutting edge: A transcriptional repressor and corepressor induced by the STAT3-regulated anti-inflammatory signaling pathway","volume":"179","author":"El Kasmi","year":"2007","journal-title":"J Immunol"},{"key":"B101","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s40246-021-00374-9","article-title":"Update of the keratin gene family: evolution, tissue-specific expression patterns, and relevance to clinical disorders","volume":"16","author":"Ho","year":"2022","journal-title":"Hum Genomics"},{"key":"B102","doi-asserted-by":"publisher","DOI":"10.1016\/0169-328X(96)00069-1","article-title":"Molecular cloning of ictacalcin: a novel calcium-binding protein from the channel catfish, Ictalurus punctatus","volume":"41","author":"Porta","year":"1996","journal-title":"Mol Brain Res"},{"key":"B103","doi-asserted-by":"publisher","DOI":"10.1002\/eji.202149383","article-title":"Identification of the Fc-alpha\/mu receptor in Xenopus provides insight into the emergence of the poly-Ig receptor (pIgR) and mucosal Ig transport","volume":"51","author":"Flowers","year":"2021","journal-title":"Eur J Immunol"},{"key":"B104","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2024.1345381","article-title":"Chemokines and lymphocyte homing in Sj\u00f6gren\u2019s syndrome","volume":"15","author":"Liao","year":"2024","journal-title":"Front Immunol"},{"key":"B105","doi-asserted-by":"publisher","first-page":"6843","DOI":"10.1038\/s41467-021-27232-5","article-title":"Single-cell analysis identifies dynamic gene expression networks that govern B cell development and transformation","volume":"12","author":"Lee","year":"2021","journal-title":"Nat Commun"},{"key":"B106","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2010.11.033","article-title":"Arachidonate 5-lipoxygenase establishes adaptive humoral immunity by controlling primary B cells and their cognate T-cell help","volume":"178","author":"Nagashima","year":"2011","journal-title":"Am J Pathol"},{"key":"B107","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0156429","article-title":"Transgelin-2 in B-cells controls T-cell activation by stabilizing T cell - B cell conjugates","volume":"11","author":"Na","year":"2016","journal-title":"PLoS One"},{"key":"B108","doi-asserted-by":"publisher","DOI":"10.1515\/hsz-2012-0131","article-title":"Platelet function and thymosin \u03b24","volume":"393","author":"Kaur","year":"2012","journal-title":"Biol Chem"},{"key":"B109","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1038\/nrn.2015.1","article-title":"Tau in physiology and pathology","volume":"17","author":"Wang","year":"2016","journal-title":"Nat Rev Neurosci"},{"key":"B110","doi-asserted-by":"publisher","first-page":"2185","DOI":"10.1038\/s41467-021-22479-4","article-title":"Therapeutic B-cell depletion reverses progression of Alzheimer\u2019s disease","volume":"12","author":"Kim","year":"2021","journal-title":"Nat Commun"},{"key":"B111","doi-asserted-by":"publisher","DOI":"10.1002\/alz.13118","article-title":"Blood tau-PT217 contributes to the anesthesia\/surgery-induced delirium-like behavior in aged mice","volume":"19","author":"Lu","year":"2023","journal-title":"Alzheimer\u2019s Dementia"},{"key":"B112","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1016\/j.fsi.2017.10.003","article-title":"Different IgM+ B cell subpopulations residing within the peritoneal cavity of vaccinated rainbow trout are differently regulated by BAFF","volume":"85","author":"Granja","year":"2019","journal-title":"Fish Shellfish Immunol"},{"key":"B113","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.1016974","article-title":"Teleost fish IgM+ plasma-like cells possess IgM-secreting, phagocytic, and antigen-presenting capacities","volume":"13","author":"Wu","year":"2022","journal-title":"Front Immunol"},{"key":"B114","doi-asserted-by":"publisher","first-page":"13600","DOI":"10.1038\/ncomms13600","article-title":"Mature IgM-expressing plasma cells sense antigen and develop competence for cytokine production upon antigenic challenge","volume":"7","author":"Blanc","year":"2016","journal-title":"Nat Commun"},{"key":"B115","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2018.7594","article-title":"Selenoprotein M promotes hypothalamic leptin signaling and thioredoxin antioxidant activity","volume":"35","author":"Gong","year":"2019","journal-title":"Antioxidants Redox Signaling"},{"key":"B116","doi-asserted-by":"publisher","first-page":"104238","DOI":"10.1016\/j.dci.2021.104238","article-title":"Identification of amphioxus protein disulfide isomerase as both an enzyme and an immunocompotent factor","volume":"126","author":"Ma","year":"2022","journal-title":"Dev Comp Immunol"},{"key":"B117","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2012.04.014","article-title":"Channel catfish (Ictalurus punctatus) protein disulphide isomerase, PDIA6: Molecular characterization and expression regulated by bacteria and virus inoculation","volume":"33","author":"Sha","year":"2012","journal-title":"Fish Shellfish Immunol"},{"key":"B118","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.4.2505","article-title":"Identification and characterization of a FcR homolog in an ectothermic vertebrate, the channel catfish (Ictalurus punctatus)","volume":"177","author":"Stafford","year":"2006","journal-title":"J Immunol"},{"key":"B119","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2018.11.001","article-title":"Insights into the dynamics of memory, effector and apoptotic cytotoxic T lymphocytes in channel catfish, Ictalurus punctatus","volume":"92","author":"Spencer","year":"2019","journal-title":"Dev Comp Immunol"}],"container-title":["Frontiers in Immunology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fimmu.2025.1547193\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T03:19:34Z","timestamp":1742181574000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fimmu.2025.1547193\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,17]]},"references-count":119,"alternative-id":["10.3389\/fimmu.2025.1547193"],"URL":"https:\/\/doi.org\/10.3389\/fimmu.2025.1547193","relation":{},"ISSN":["1664-3224"],"issn-type":[{"value":"1664-3224","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,17]]},"article-number":"1547193"}}