{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T12:57:10Z","timestamp":1780405030263,"version":"3.54.1"},"reference-count":56,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T00:00:00Z","timestamp":1753833600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>Sepsis, particularly sepsis-induced liver injury (SLI), exhibits acute onset and high mortality (up to 80%). While murine models are widely used for mechanistic studies due to limited human sample availability, their accuracy in replicating human SLI pathophysiology remains debated. <\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Human SLI transcriptomes were characterized to identify core genes and immune signatures using Venn analysis and immune infiltration profiling. Transcriptomic features of two murine SLI models\u2014cecal ligation and puncture (CLP) and lipopolysaccharide (LPS) challenge\u2014were benchmarked against human SLI to evaluate pathophysiological relevance. Both models were then utilized to validate core gene expression for SLI biomarker identification. <\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Human SLI transcriptomics revealed significant enrichment in apoptotic processes, NF-\u03baB regulation, inflammatory responses, protein phosphorylation, and bacterial response. Key pathways included IL-17 signaling, antigen processing, estrogen signaling, and atherosclerosis. Immune infiltration confirmed multifactorial immune cell involvement. Both murine models recapitulated inflammatory and immune responses, with the LPS model mimicking human SLI via chemotaxis, phagocytosis, NOD-like receptor signaling, and leukocyte migration. The CLP model uniquely replicated neutrophil chemotaxis, apoptosis, ER stress, IL-17, and TNF signaling. SOCS3 was validated as a potential SLI biomarker. <\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>Murine models partially replicate human SLI pathology but exhibit distinct mechanistic emphases. Careful model selection is essential for biomarker discovery (e.g., SOCS3) and pathogenic mechanism exploration, highlighting inherent model limitations.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fimmu.2025.1556392","type":"journal-article","created":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T05:28:06Z","timestamp":1753853286000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["Transcriptional responses in different mouse models of septic liver injury differ from those in patients with septic liver injury"],"prefix":"10.3389","volume":"16","author":[{"given":"Qin","family":"Yan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Fan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinsen","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shi","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolin","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,7,30]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2016.0287","article-title":"The third international consensus definitions for sepsis and septic shock (Sepsis-3)","volume":"315","author":"Singer","year":"2016","journal-title":"JAMA"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.16604","article-title":"Wogonin alleviates liver injury in sepsis through Nrf2-mediated NF-\u03baB signalling suppression","volume":"25","author":"Dai","year":"2021","journal-title":"J Cell Mol Med"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.5847\/wjem.j.1920-8642.2022.056","article-title":"Trichostatin A improves the inflammatory response and liver injury in septic mice through the FoxO3a\/autophagy signaling pathway","volume":"13","author":"Shen","year":"2022","journal-title":"World J Emerg Med"},{"key":"B4","doi-asserted-by":"publisher","first-page":"282","DOI":"10.1097\/SHK.0000000000000495","article-title":"Workgroup O behalf of the AX","volume":"45","author":"Rimmel\u00e9","year":"2016","journal-title":"Immune Cell Phenotype Funct Sepsis. 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