{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T13:06:03Z","timestamp":1783602363749,"version":"3.55.0"},"reference-count":49,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,3,19]],"date-time":"2025-03-19T00:00:00Z","timestamp":1742342400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>The pathogenesis of inflammatory bowel diseases (IBD) involves genetic, environmental, immunological, and microbial factors; however, it remains unclear. Pro-inflammatory interleukin 8 (IL-8), encoded by the <jats:italic>CXCL8<\/jats:italic> gene, assumes a crucial chemotactic role in leukocyte migration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This study aimed to investigate whether an association exists between IBD and two <jats:italic>CXCL8<\/jats:italic> variants, namely, c.-251A&amp;gt;T (rs4073) and c.91G&amp;gt;T (rs188378669), and IL-8 concentration. We analyzed the distribution of both variants among 353 Polish IBD patients and 200 population subjects using pyrosequencing, competitive allele-specific PCR and Sanger sequencing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The c.91T stop-gained allele was significantly more frequent in IBD patients (2.12%) than in controls (0.25%) (<jats:italic>p<\/jats:italic> = 0.0121), while the c.-251T allele frequencies were similar (54% vs. 51.5%, <jats:italic>p<\/jats:italic> = 0.4955). Serum IL-8 concentrations, measured using ELISA, were higher in IBD patients with the c.91 GG genotype compared to healthy controls (mean, 70.02 vs. 51.5 pg\/ml, <jats:italic>p<\/jats:italic>&amp;lt;0.01) and patients with c.91 GT (mean, 61.73 pg\/ml). Moreover, clinical data indicated that carriers of the c.91T variant need more often corticosteroids and surgical treatment of the disease than GG homozygous IBD patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This suggest that the <jats:italic>CXCL8<\/jats:italic> c.91T allele may influence IBD manifestation and the course of the disorders in Polish patients, potentially serving as a novel target for future studies and therapeutic approaches.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fimmu.2025.1562618","type":"journal-article","created":{"date-parts":[[2025,3,19]],"date-time":"2025-03-19T05:16:30Z","timestamp":1742361390000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Is a rare CXCL8 gene variant a new possible cause or course factor of inflammatory bowel 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