{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T18:39:46Z","timestamp":1792521586781,"version":"4.5.0"},"reference-count":49,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,4,15]],"date-time":"2025-04-15T00:00:00Z","timestamp":1744675200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>During orthodontic tooth movement, sterile inflammatory processes and alveolar bone resorption occur in the periodontal ligament, involving myeloid cells such as macrophages and osteoclasts. The myeloid p38\u03b1\/MAPK (mitogen-activated protein kinase) not only regulates the inflammatory response of macrophages and osteoclast differentiation but also the activation of the osmoprotective transcription factor NFAT5 (nuclear factor of activated T cells 5) under high-salt conditions. Therefore, this study aims to investigate the relative role of myeloid p38\u03b1\/MAPK in orthodontic tooth movement as a function of extracellular salt content.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and methods<\/jats:title><jats:p>Macrophages and osteoclasts were differentiated from the bone marrow of mice lacking p38\u03b1\/MAPK expression in myeloid cells (<jats:italic>p38\u03b1<\/jats:italic><jats:sup>\u0394myel<\/jats:sup>) and controls for RNA analysis and calcium phosphate resorption assay. Controls and <jats:italic>p38\u03b1<\/jats:italic><jats:sup>\u0394myel<\/jats:sup> mice were fed a low or a high salt diet for a total of two weeks. One week after the start of the diet, an elastic band was inserted between the first and second molar to induce orthodontic tooth movement. Atomic absorption spectrometry was used to assess the sodium balance of the jaw bone tissue. RNA was isolated from the periodontium of the first molar, osteoclast numbers and extent of orthodontic tooth movement were assessed. <\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:italic>Nfat5<\/jats:italic> mRNA was increased in macrophages and osteoclasts <jats:italic>in vitro<\/jats:italic> and in the periodontium <jats:italic>in vivo<\/jats:italic> after high salt treatment in control mice but not in <jats:italic>p38\u03b1<\/jats:italic><jats:sup>\u0394myel<\/jats:sup> mice. While there was no salt effect on interleukin-6 (<jats:italic>Il6<\/jats:italic>) gene expression, prostaglandin endoperoxide synthase-2 (<jats:italic>Ptgs2<\/jats:italic>) mRNA was upregulated in control but not in <jats:italic>p38\u03b1<\/jats:italic><jats:sup>\u0394myel<\/jats:sup> mice <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic>. p38\u03b1\/MAPK deletion increased osteoclast numbers after low and high salt diet. Of note, deletion of p38\u03b1\/MAPK elevated osteoclast activity under control salt conditions but reduced osteoclast activity under high salt conditions. High-salt diet resulted in increased sodium ion deposition in the jaw of both genotypes, while tooth movement was only increased in control mice. In <jats:italic>p38\u03b1<\/jats:italic><jats:sup>\u0394myel<\/jats:sup> mice, high salt diet reduced the extent of orthodontic tooth movement, which could be explained by the reduced bone resorption of osteoclasts.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We conclude that myeloid p38\u03b1\/MAPK promotes macrophage <jats:italic>Ptgs2<\/jats:italic> expression and osteoclast activity in response to extracellular salt levels, thereby supporting orthodontic tooth movement.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fimmu.2025.1571268","type":"journal-article","created":{"date-parts":[[2025,4,15]],"date-time":"2025-04-15T13:21:01Z","timestamp":1744723261000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["During high salt treatment myeloid p38\u03b1\/MAPK fosters osteoclast activity and inflammatory macrophage responses promoting orthodontic tooth 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