{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,19]],"date-time":"2026-07-19T04:17:17Z","timestamp":1784434637130,"version":"3.55.0"},"reference-count":37,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,6,25]],"date-time":"2025-06-25T00:00:00Z","timestamp":1750809600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:p>\n                    Paneth cells are located in the crypts of Lieberk\u00fchn in mammalian small intestines and are producing antimicrobial peptides to keep the microbiome under control. The genetic manipulation of Paneth cells and their tracking and depletion depend on a solid Paneth cell\u2013specific\n                    <jats:italic>Cre<\/jats:italic>\n                    -transgenic line. Here, we describe bulk RNA sequencing (RNA-seq)\u2013based expression data from pure, sorted Paneth cells of C57BL\/6J mice and identify several strongly expressed Paneth cell\u2013specific genes, the expression of which is stable under pathophysiological conditions, as well as in the duodenum, jejunum, and ileum. We selected the\n                    <jats:italic>Defa24<\/jats:italic>\n                    gene regulatory sequences and generated a new\n                    <jats:italic>Defa24iCre<\/jats:italic>\n                    transgenic line using BAC technology, Tg(Defa24-icre)Cli. The resulting transgenic line provides robust expression and allows for the complete depletion of Paneth cells by cell ablation, yielding mice without any detectable lysozyme biological activity in the small intestines.\n                  <\/jats:p>","DOI":"10.3389\/fimmu.2025.1576995","type":"journal-article","created":{"date-parts":[[2025,6,25]],"date-time":"2025-06-25T01:26:31Z","timestamp":1750814791000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Generation of a new Paneth cell\u2013specific Cre-recombinase transgenic mouse line"],"prefix":"10.3389","volume":"16","author":[{"given":"Natalia","family":"Garcia-Gonzalez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte","family":"Wallaeys","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wendy","family":"Toussaint","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tino","family":"Hochepied","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylviane","family":"Dewaele","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Somara","family":"De Beul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robin","family":"Van 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