{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T11:22:51Z","timestamp":1793532171825,"version":"4.5.0"},"reference-count":26,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,6,18]],"date-time":"2025-06-18T00:00:00Z","timestamp":1750204800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100012542","name":"Sichuan Provincial Science and Technology Support Program","doi-asserted-by":"publisher","award":["2023NSFSC0586"],"award-info":[{"award-number":["2023NSFSC0586"]}],"id":[{"id":"10.13039\/100012542","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>Immunoglobulin Y (IgY) has emerged as a promising antibody therapy for the eradication of <jats:italic>Helicobacter pylori<\/jats:italic> (<jats:italic>H. pylori<\/jats:italic>) independent of antibiotics. However, the roles and differences of IgY antibodies targeting various genes against <jats:italic>H. pylori<\/jats:italic> remain unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The recombinant antigens of five colonization-related genes \u2014 <jats:italic>FlaA<\/jats:italic>, <jats:italic>BabA2<\/jats:italic>, <jats:italic>NapA<\/jats:italic>, <jats:italic>HpaA<\/jats:italic>, and <jats:italic>UreB<\/jats:italic> \u2014 are prepared using a prokaryotic expression system and then subject to immunize laying hens for IgY production. Subsequently, their biological activities are evaluated, including blocking bacterial growth, attenuating infection in GES-1 cells, and eradicating <jats:italic>H. pylori<\/jats:italic> in gastritis mouse models.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>These IgY antibodies can recognize the full-length antigens of <jats:italic>H. pylori<\/jats:italic> and exhibit a direct inhibitory effect on the growth and infection of <jats:italic>H. pylori<\/jats:italic> with dose-dependent characteristics. Among these, anti-<jats:italic>FlaA<\/jats:italic> IgY shows greater antibacterial activity in inhibiting <jats:italic>H. pylori<\/jats:italic> growth and preventing adhesion to GES-1 cells. Oral administration of these IgY antibodies for two weeks (20.0 mg\u00b7kg<jats:sup>\u22121<\/jats:sup>\u00b7day<jats:sup>\u22121<\/jats:sup>) achieves a 25% to 37.5% eradication rate of <jats:italic>H. pylori<\/jats:italic> infection in mice. Interestingly, combination treatment with these IgY antibodies, based on their different roles, enhances antibacterial benefits and significantly promotes the recovery of gastrointestinal function.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our results indicate that IgY antibodies against colonization-related genes can directly block the growth and infection of <jats:italic>H. pylori<\/jats:italic>, and combination treatment with these antibodies offers more advantages in combating <jats:italic>H. pylori<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fimmu.2025.1582250","type":"journal-article","created":{"date-parts":[[2025,6,18]],"date-time":"2025-06-18T05:47:07Z","timestamp":1750225627000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":7,"title":["Immunoglobulin Y antibodies against colonization-related genes block the growth and infection of Helicobacter pylori"],"prefix":"10.3389","volume":"16","author":[{"given":"Shiyuan","family":"Deng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoling","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunxiao","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rania G.","family":"Elbaiomy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weihan","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed H.","family":"El-Sappah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohong","family":"Jian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongmei","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed","family":"Bakeer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zaixin","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,6,18]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"401","DOI":"10.1186\/s12866-023-03143-x","article-title":"Reduction of UreB and CagA expression level by siRNA construct in Helicobacter pylori strain SS1","volume":"23","author":"Motamedi","year":"2023","journal-title":"BMC Microbiol"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.1034683","article-title":"Protective immunity enhanced Salmonella vaccine vectors delivering Helicobacter pylori antigens reduce H. pylori stomach colonization in mice","volume":"13","author":"Ghasemi","year":"2022","journal-title":"Front Immunol"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2010.07393.x","article-title":"Helicobacter pylori HP0518 affects flagellin glycosylation to alter bacterial motility","volume":"78","author":"Asakura","year":"2010","journal-title":"Mol Microbiol"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v19.i26.4242","article-title":"Association of Helicobacter pylori babA2 with peptic ulcer disease and gastric cancer","volume":"19","author":"Chen","year":"2013","journal-title":"World J Gastroenterol"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1111\/hel.12758","article-title":"Helicobacter pylori adhesins: HpaA a potential antigen in experimental vaccines for H","volume":"26","author":"Banga Ndzouboukou","year":"2021","journal-title":"pylori. 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