{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T14:06:02Z","timestamp":1789049162521,"version":"build-2803163510"},"reference-count":108,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,5,5]],"date-time":"2025-05-05T00:00:00Z","timestamp":1746403200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100013126","name":"Psoriasis Association","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100013126","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Psoriasis is a systemic, immune-mediated, inflammatory skin disease in which T cells have been found to play a significant role. The phenotypic and functional properties of circulating CD8 T cells in the pathogenesis of the disease are still ill-defined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>This study aimed to assess changes in the phenotype, activation status and mediator release of CD8 T cells in the peripheral blood of patients with mild-to-moderate psoriasis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Peripheral blood mononuclear cells from patients with mild-to-moderate psoriasis and healthy individuals were used to investigate the CD8 T cell immune phenotype and mediator release upon <jats:italic>in vitro<\/jats:italic> TCR-independent (phorbol 12-myristate 13-acetate (PMA) plus ionomycin (ION)) or TCR-dependent (anti-CD3\/CD28) activation by flow cytometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Patients with psoriasis exhibited reduced circulating CD8 memory T cell frequency compared to healthy controls. Additionally, although CD8 T cell subsets showed similar levels of the skin homing marker CCR4, they demonstrated a significant upregulation of B- and T-lymphocyte attenuator (BTLA) expression compared to healthy individuals. Upon CD8 T cell activation, IL-17A and IL-17F were expressed at low and comparable levels in psoriasis patients and healthy controls. In contrast, CD69, IFN\u03b3, and Granzyme B were significantly decreased in anti-CD3\/CD28-activated CD8 T cell subsets. PASI scores positively correlated with IFN\u03b3-producing CD8 T memory cells and negatively with TNF-producing CD8- T cell subsets.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Patients with mild-to-moderate psoriasis showed a significant decrease in CD8 T memory cells and reduced release of cytotoxic mediators by CD8 T cells. Thus, this indicates that psoriasis impacts the functionality of circulating CD8 T cells.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fimmu.2025.1585378","type":"journal-article","created":{"date-parts":[[2025,5,5]],"date-time":"2025-05-05T05:10:50Z","timestamp":1746421850000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":5,"title":["Circulating CD8 T cells from patients with mild-to-moderate psoriasis are functionally impaired"],"prefix":"10.3389","volume":"16","author":[{"given":"Yiqiao","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Tontini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabella","family":"Tosi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan N.","family":"Barker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Di Meglio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher E. 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