{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T10:02:40Z","timestamp":1771927360458,"version":"3.50.1"},"reference-count":38,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T00:00:00Z","timestamp":1769126400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Numerous studies point to a role for the immune system in various animal models of hypertension. However, little is known about the immune system of Bph\/2 mice, a spontaneously hypertensive strain.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>To address this, we conducted a comprehensive comparison of immune cell composition and response to polyclonal T cell activation in hypertensive Bph\/2 mice and normotensive Bpn\/3 control mice. We quantified immune cell populations by flow cytometry from spleen and inguinal, brachial and mesenteric lymph nodes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      While composition of myeloid immune cell types was largely comparable between strains, we observed differences in B and T cell subpopulations. Specifically, we found an increased percentage of IgM+ IgD\n                      <jats:sup>Lo<\/jats:sup>\n                      and IgM+ IgD- B cells in Bph\/2 mice, suggesting greater baseline B cell activation or differences in B cell lineage. In addition, we observed a decreased percentage of CD4 effector memory T cells and CD8 central memory T cells. The diminished proportion of memory T cells in Bph\/2 mice correlated with decreased proliferation and cytokine response of splenic T cells to polyclonal T cell activation. In splenic T cells from Bph\/2 mice 24 h after activation, we observed a pronounced decrease in the majority of T cell cytokines. At 120 h after activation, the Th1 and Th17 cytokine responses of splenic T cells from Bph\/2 mice were decreased, but other T cell cytokines were largely comparable between genotypes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion\/Discussion<\/jats:title>\n                    <jats:p>Overall, the data suggest a decreased percentage of memory T cells in Bph\/2 mice that correlates with markedly diminished proliferation and cytokine response to polyclonal activation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fimmu.2025.1598843","type":"journal-article","created":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T06:48:41Z","timestamp":1769150921000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Differential composition of lymphocyte subpopulations and activation between the hypertensive Bph\/2 and normotensive Bpn\/3 mouse strains"],"prefix":"10.3389","volume":"16","author":[{"given":"Devon","family":"Dattmore","sequence":"first","affiliation":[{"name":"Applied Immunology Center for Education and Research, Michigan State University","place":["East Lansing, MI, United States"]},{"name":"Department of Pharmacology and Toxicology, Michigan State University","place":["East Lansing, MI, United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paiton O.","family":"McDonald","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Michigan State University","place":["East Lansing, MI, United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Afrin","family":"Chowdhury","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Michigan State University","place":["East Lansing, MI, United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saamera","family":"Awali","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Michigan State University","place":["East Lansing, MI, United States"]},{"name":"Integrated Pharmacological Sciences Training Program, Michigan State University","place":["East Lansing, MI, United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Allison P.","family":"Boss","sequence":"additional","affiliation":[{"name":"Applied Immunology Center for Education and Research, Michigan State University","place":["East Lansing, MI, United States"]},{"name":"Department of Food Science and Human Nutrition, Michigan State University","place":["East Lansing, MI, United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yining","family":"Jin","sequence":"additional","affiliation":[{"name":"Applied Immunology Center for Education and Research, Michigan State University","place":["East Lansing, MI, United States"]},{"name":"Department of Pharmacology and Toxicology, Michigan State University","place":["East Lansing, MI, United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa","family":"Sather","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Michigan State University","place":["East Lansing, MI, United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. 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