{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T14:22:23Z","timestamp":1780582943646,"version":"3.54.1"},"reference-count":42,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T00:00:00Z","timestamp":1759449600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:p>A subset of people with HIV, termed HIV controllers (HIC), maintain low viral loads without antiretroviral therapy. To identify the immune cell architecture of HIV control, we profiled peripheral blood from 54 HIC (including 21 elite controllers, EC) and 1,044 non-controllers (non-HIC) in the 2000HIV study (NCT03994835) using high-dimensional cytometry and confounder-adjusted regression analysis. Both HIC and EC exhibited distinct innate immune profiles compared to non-HIC, marked by reduced frequencies of CCR5<jats:sup>+<\/jats:sup> NKT and TCR\u03b3\u03b41<jats:sup>+<\/jats:sup> cells. EC further showed increased neutrophils and TCR\u03b3\u03b42<jats:sup>+<\/jats:sup> cells, and reduced eosinophils. Unsupervised clustering revealed elevated CD11c and CD1c expression on TCR\u03b3\u03b42<jats:sup>+<\/jats:sup> cells in EC, correlating with IFN\u03b3 production, suggesting a proinflammatory \u03b3\u03b4 T cell program unique to EC. Adaptive immune profiling showed shared features between HIC and EC: increased CD4<jats:sup>+<\/jats:sup> na\u00efve and Th1\/17 cells, reduced Th17 and Tfh cells, and higher CD8<jats:sup>+<\/jats:sup> TEMRA and Tc1\/17 cells with reduced memory subsets. Both groups showed increased na\u00efve and immature B cells and decreased switched memory and plasma cells. EC uniquely exhibited increased IgA<jats:sup>+<\/jats:sup> memory B cells \u2014a feature consistent with enhanced mucosal immunity\u2014 and decreased IgG<jats:sup>+<\/jats:sup> memory B cells and CD307d expression, suggestive of mucosal imprinting and reduced exhaustion. Comparison of HIC and EC revealed divergent CCR5 and CXCR4 expression: EC had higher frequencies of CCR5<jats:sup>+<\/jats:sup> and CXCR4<jats:sup>+<\/jats:sup> CD4<jats:sup>+<\/jats:sup> and CD8<jats:sup>+<\/jats:sup> T cells. These elevations correlated with circulating chemokines, notably MIF for CXCR4, implying protective ligand occupancy. HIC instead showed overall lower co-receptor expression and ligand correlations. In conclusion, while HIC and EC share a core immune phenotype linked to viral control, EC-specific features\u2014 \u03b3\u03b4 T cell activation, IgA<jats:sup>+<\/jats:sup> memory enrichment, and chemokine receptor regulation\u2014may underlie more robust or distinct immune control mechanisms. This profiling resource offers new avenues for HIV cure-focused strategies.<\/jats:p>","DOI":"10.3389\/fimmu.2025.1642482","type":"journal-article","created":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T12:29:21Z","timestamp":1759494561000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["Circulating immune landscape and immune signatures in spontaneous HIV controllers"],"prefix":"10.3389","volume":"16","author":[{"given":"Adriana","family":"Navas","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9ssica C.","family":"dos Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bram","family":"van Cranenbroek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadira","family":"Vadaq","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Albert L.","family":"Groenendijk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilhelm A. 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