{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:17:00Z","timestamp":1787026620708,"version":"build-2736575974"},"reference-count":73,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T00:00:00Z","timestamp":1756771200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002923","name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","doi-asserted-by":"publisher","award":["PIP3022"],"award-info":[{"award-number":["PIP3022"]}],"id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:p>\n                    Acellular pertussis (aP) vaccines have markedly reduced the global burden of severe pertussis. However, their limited ability to elicit mucosal and durable immunity has been linked to waning protection and sustained\n                    <jats:italic>Bordetella pertussis<\/jats:italic>\n                    circulation. Selective pressure exerted by widespread aP vaccination has contributed to the emergence and regional dissemination of pertactin-deficient (PRN\n                    <jats:sup>\u2212<\/jats:sup>\n                    ) strains, raising additional concerns regarding vaccine effectiveness. In this context, we investigated whether incorporating outer membrane vesicles (OMVs) derived from\n                    <jats:italic>B. pertussis<\/jats:italic>\n                    into the aP vaccine could enhance its immunological profile, specifically by promoting Th1\/Th17 polarization, inducing tissue-resident memory (TRM) T cells, and broadening protective coverage to include PRN\n                    <jats:sup>\u2212<\/jats:sup>\n                    isolates, while maintaining aP-induced immunity against lower respiratory tract colonization. Using a murine intranasal challenge model with a two-dose vaccination schedule, we assessed the safety, immunogenicity, and protective capacity of the OMV+aP vaccine prototype (combined) versus aP vaccine. The combined formulation was well tolerated and induced robust systemic and mucosal responses, characterized by higher IgG2a\/IgG1 ratios, increased Th1\/Th17 cytokine production (IFN-\u03b3, IL - 17, and IL - 22), and elevated anti-\n                    <jats:italic>B. pertussis<\/jats:italic>\n                    IgA titers. Flow cytometric analyses revealed lung- and nasal-resident CD4\n                    <jats:sup>+<\/jats:sup>\n                    TRM cells in the combined immunized mice, which were absent in those receiving aP alone. Functionally, OMV+aP formulation conferred superior protection in pulmonary and nasal compartments, significantly reducing lung bacterial loads (including against PRN\n                    <jats:sup>\u2212<\/jats:sup>\n                    strains) and uniquely diminishing nasal colonization even under high-dose challenge conditions. Passive transfer experiments confirmed the role of cellular and humoral immunity in bacterial clearance. These results demonstrate that OMVs synergize with aP to enhance immune response magnitude and quality, addressing key gaps in current aP vaccines and offering a next-generation strategy to prevent both disease and transmission.\n                  <\/jats:p>","DOI":"10.3389\/fimmu.2025.1655910","type":"journal-article","created":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T05:34:20Z","timestamp":1756791260000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Harnessing outer membrane vesicles derived from Bordetella pertussis to overcome key limitations of acellular pertussis vaccines"],"prefix":"10.3389","volume":"16","author":[{"given":"Luc\u00eda","family":"Locati","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Bottero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco","family":"Carriquiriborde","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oriana","family":"L\u00f3pez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernarda","family":"Pschunder","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eugenia","family":"Zurita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo","family":"Martin Aispuro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Emilia","family":"Gaillard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Hozbor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,9,2]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1097\/01.inf.0000177282.53500.77","article-title":"Resurgence of pertussis in europe","volume":"24","author":"Celentano","year":"2005","journal-title":"Pediatr Infect Dis J"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMp1209051","article-title":"Epidemic pertussis in 2012 \u2014 The resurgence of a vaccine-preventable disease","volume":"367","author":"Cherry","year":"2012","journal-title":"New Engl J Med"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.19-0013","article-title":"Resurgence of pertussis infections in Shandong, China: Space-time cluster and trend analysis","volume":"100","author":"Zhang","year":"2019","journal-title":"Am J Trop Med Hygiene"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.2807\/esw.10.33.02779-en","article-title":"Pertussis outbreak in recently vaccinated children in a kindergarten in Ljubljana during a resurgence in pertussis incidence","volume":"10","author":"Grilc","year":"2005","journal-title":"Euro Surveill"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.4415\/ANN_17_02_04","article-title":"Pertussis in infants and the resurgence of a vaccine preventable disease: What to do","volume":"53","author":"Fedele","year":"2017","journal-title":"Ann Ist Super Sanita"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2024.29.14.2400160","article-title":"Pertussis epidemic in Denmark, august 2023 to february 2024","volume":"29","author":"Nordholm","year":"2024","journal-title":"Euro Surveill"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1093\/CID\/CIT934","article-title":"The pertussis problem","volume":"58","author":"Plotkin","year":"2014","journal-title":"Clin Infect Dis"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1016\/J.IJID.2024.107148","article-title":"Recent increase in infant pertussis cases in Europe and the critical importance of antenatal immunizations: We must do better \u2026 now","volume":"146","author":"Khalil","year":"2024","journal-title":"Int J Infect Dis"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1016\/J.JINF.2024.106219","article-title":"Pertussis in infants: Alarm lights and amplifiers for persistent community transmission","volume":"89","author":"Meng","year":"2024","journal-title":"J Infect"},{"key":"B10","doi-asserted-by":"publisher","first-page":"q1545","DOI":"10.1136\/BMJ.Q1545","article-title":"Whooping cough: Nine infants have died in England in latest outbreak","volume":"386","author":"Kmietowicz","year":"2024","journal-title":"BMJ"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1080\/21645515.2015.1113357","article-title":"The resurgence of mumps and pertussis","volume":"12","author":"Sabbe","year":"2016","journal-title":"Hum Vaccin Immunother"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1008625","article-title":"Whoop! 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