{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T12:31:06Z","timestamp":1793363466700,"version":"4.5.0"},"reference-count":60,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T00:00:00Z","timestamp":1764201600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003042","name":"Else Kr\u00f6ner-Fresenius-Stiftung","doi-asserted-by":"publisher","award":["#2017_A51"],"award-info":[{"award-number":["#2017_A51"]}],"id":[{"id":"10.13039\/501100003042","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005972","name":"Deutsche Krebshilfe","doi-asserted-by":"publisher","award":["#70112951"],"award-info":[{"award-number":["#70112951"]}],"id":[{"id":"10.13039\/501100005972","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      Effective CAR T cell infiltration into solid tumors remains a major barrier to therapy success. Despite their clinical potential, few studies have evaluated phenotypes of CAR T cells successfully invading the tumor mass following infusion. Phenotypic information would enrich our understanding of the mechanisms governing CAR T cell migration into solid tumors. Here we implemented an\n                      <jats:italic>in vitro<\/jats:italic>\n                      strategy to identify genes driving L1CAM-CAR T cell migration into a 3D tumor mass.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>L1CAM-CAR T cells were separated into 2 groups by their capability to infiltrate (or not) a 3D bioprinted neuroblastoma model. Single-cell and bulk RNA sequencing was performed, and infiltrating CAR T cells were compared to noninfiltrating cells to seek genetic drivers of CAR T cell migration. CRISPR\/Cas9 technology was used to generate modified L1CAM-CAR T cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Tumor-infiltrating L1CAM-CAR T cells expressed lower levels of the selectin P ligand (SELPLG) glycoprotein and higher levels of the T cell-specific adaptor protein, SH2D2A. Functional characterization of L1CAM-CAR T cells genetically modified to enforce these characteristics demonstrated that neither trait negatively impacted L1CAM-CAR T cell cytotoxicity, activation and cytokine release upon coculture with neuroblastoma target cells. Transgenic SH2D2A expression did not improve CAR T cell migration in an endothelial transmembrane assay. SELPLG knockout benefited CAR T cell\n                      <jats:italic>in vitro<\/jats:italic>\n                      trans-endothelial migration, but did not enhance anti-tumor efficacy in an immunodeficient mouse model.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>\n                      Our findings reveal a key limitation of murine xenograft models, which are widely used as the gold standard for preclinical CAR T cell testing. The lack of conservation between the human and murine SELPLG proteins likely accounts for the discrepancy between enhanced\n                      <jats:italic>in vitro<\/jats:italic>\n                      migration of SELPLG-deficient L1CAM-CAR T cells and their lack of improved efficacy in the mouse model. This underscores the need for more predictive human-relevant models to better preclinically evaluate CAR T cell function.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fimmu.2025.1677361","type":"journal-article","created":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T09:54:47Z","timestamp":1764237287000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["Investigating genetic modifications to enhance L1CAM-CAR T cell migration in solid tumors in a 3D bioprinted neuroblastoma model"],"prefix":"10.3389","volume":"16","author":[{"given":"Lena","family":"Andersch","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Grunewald","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Stecklum","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filippos","family":"Klironomos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kerstin","family":"Haase","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viola","family":"Hollek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias","family":"Lam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beate Anahita","family":"Jung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anika","family":"Winkler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silke","family":"Schwiebert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathy","family":"Astrahantseff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Launspach","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marvin","family":"Jens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anton","family":"Henssen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lutz","family":"Kloke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nils","family":"Bl\u00fcthgen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelika","family":"Eggert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes H.","family":"Schulte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathleen","family":"Anders","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annette","family":"K\u00fcnkele","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,11,27]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra0804577","article-title":"Recent Advances in Neuroblastoma","volume":"362","author":"Maris","year":"2010","journal-title":"New Engl J Med"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.28748","article-title":"Declining Childhood and Adolescent Cancer Mortality","volume":"120","author":"Smith","year":"2014","journal-title":"Cancer"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-05-354449","article-title":"Antitumor Activity and Long-Term Fate of Chimeric Antigen Receptor-Positive T Cells in Patients with Neuroblastoma","volume":"118","author":"Louis","year":"2011","journal-title":"Blood"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2210859","article-title":"Gd2-Cart01 for Relapsed or Refractory High-Risk Neuroblastoma","volume":"388","author":"Del Bufalo","year":"2023","journal-title":"New Engl J Med"},{"key":"B5","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1126\/scitranslmed.abd6169","article-title":"Antitumor Activity without on-Target Off-Tumor Toxicity of Gd2\u2013Chimeric Antigen Receptor T Cells in Patients with Neuroblastoma","volume":"12","author":"Straathof","year":"2020","journal-title":"Sci Transl Med"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2017.05.012","article-title":"Car T Cells Administered in Combination with Lymphodepletion and Pd-1 Inhibition to Patients with Neuroblastoma","volume":"25","author":"Heczey","year":"2017","journal-title":"Mol Ther"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.128.6.1243","article-title":"The Integrin Vla-4 Supports Tethering and Rolling in Flow on Vcam-1","volume":"128","author":"Alon","year":"1995","journal-title":"J Cell Biol"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90491-3","article-title":"\u03b14 Integrins Mediate Lymphocyte Attachment and Rolling under Physiologic Flow","volume":"80","author":"Berlin","year":"1995","journal-title":"Cell"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1084\/jem.183.3.1119","article-title":"Cd44 and Its Ligand Hyaluronate Mediate Rolling under Physiologic Flow: A Novel Lymphocyte-Endothelial Cell Primary Adhesion Pathway","volume":"183","author":"DeGrendele","year":"1996","journal-title":"J Exp Med"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1100935","article-title":"In Vivo Analysis of Uropod Function During Physiological T Cell Trafficking","volume":"187","author":"Soriano","year":"2011","journal-title":"J Immunol"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0075151","article-title":"Activated T Cell Trans-Endothelial Migration Relies on Myosin-Iia Contractility for Squeezing the Cell Nucleus through Endothelial Cell Barriers","volume":"8","author":"Jacobelli","year":"2013","journal-title":"PloS One"},{"key":"B12","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1038\/nri.2015.16","article-title":"T Cell Migration, Search Strategies and Mechanisms","volume":"16","author":"Krummel","year":"2016","journal-title":"Nat Rev Immunol"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-06-1183","article-title":"A Phase I Study on Adoptive Immunotherapy Using Gene-Modified T Cells for Ovarian Cancer","volume":"12","author":"Kershaw","year":"2006","journal-title":"Clin Cancer Res"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1136\/jitc-2023-007162","article-title":"Intratumoral Pan-Erbb Targeted Car-T for Head and Neck Squamous Cell Carcinoma: Interim Analysis of the T4 Immunotherapy Study","volume":"11","author":"Papa","year":"2023","journal-title":"J Immunother Cancer"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-04489-4","article-title":"Gd2-Car T Cell Therapy for H3k27m-Mutated Diffuse Midline Gliomas","volume":"603","author":"Majzner","year":"2022","journal-title":"Nature"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-022-01726-1","article-title":"Psma-Targeting Tgfbeta-Insensitive Armored Car T Cells in Metastatic Castration-Resistant Prostate Cancer: A Phase 1 Trial","volume":"28","author":"Narayan","year":"2022","journal-title":"Nat Med"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-16-0354","article-title":"Preclinical Assessment of Cd171-Directed Car T-Cell Adoptive Therapy for Childhood Neuroblastoma: Ce7 Epitope Target Safety and Product Manufacturing Feasibility","volume":"23","author":"K\u00fcnkele","year":"2017","journal-title":"Clin Cancer Res"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.689697","article-title":"A Reproducible Bioprinted 3d Tumor Model Serves as a Preselection Tool for Car T Cell Therapy Optimization","volume":"12","author":"Grunewald","year":"2021","journal-title":"Front Immunol"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1158\/2326-6066.CIR-14-0200","article-title":"Functional Tuning of Cars Reveals Signaling Threshold above Which Cd8+ Ctl Antitumor Potency Is Attenuated Due to Cell Fas-Fasl-Dependent Aicd","volume":"3","author":"Kunkele","year":"2015","journal-title":"Cancer Immunol Res"},{"key":"B20","first-page":"32","article-title":"Production of Cgmp-Grade Lentiviral Vectors","volume":"10","author":"Ausubel","year":"2012","journal-title":"Bioprocess Int"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-02-337360","article-title":"A Transgene-Encoded Cell Surface Polypeptide for Selection, in Vivo Tracking, and Ablation of Engineered Cells","volume":"118","author":"Wang","year":"2011","journal-title":"Blood"},{"key":"B22","doi-asserted-by":"publisher","first-page":"689","DOI":"10.1097\/CJI.0b013e318270dec7.Phenotypic","article-title":"Phenotypic and Functional Attributes of Lentivirus Modified Cd19-Specific Human Cd8+ Central Memory T Cells Manufactured at Clinical Scale","volume":"35","author":"Wang","year":"2012","journal-title":"J Immunother"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1126\/science.abe6474","article-title":"Pan-Cancer Single-Cell Landscape of Tumor-Infiltrating T Cells","volume":"374","author":"Zheng","year":"2021","journal-title":"Science"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra052723","article-title":"The Many Roles of Chemokines and Chemokine Receptors in Inflammation","volume":"354","author":"Charo","year":"2006","journal-title":"New Engl J Med"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20061706","article-title":"Ccr7 Ligands Stimulate the Intranodal Motility of T Lymphocytes in Vivo","volume":"204","author":"Worbs","year":"2007","journal-title":"J Exp Med"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.00952","article-title":"Lfa-1 in T Cell Migration and Differentiation","volume":"9","author":"Walling","year":"2018","journal-title":"Front Immunol"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2172-7-15","article-title":"Structure Function Analysis of Sh2d2a Isoforms Expressed in T Cells Reveals a Crucial Role for the Proline Rich Region Encoded by Sh2d2a Exon 7","volume":"7","author":"Granum","year":"2006","journal-title":"BMC Immunol"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20051637","article-title":"Essential Role of the T Cell-Specific Adapter Protein in the Activation of Lck in Peripheral T Cells","volume":"203","author":"Marti","year":"2006","journal-title":"J Exp Med"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1084\/jem.193.12.1425","article-title":"A Transcription Function for the T Cell\u2013Specific Adapter (Tsad) Protein in T Cells: Critical Role of the Tsad Src Homology 2 Domain","volume":"193","author":"Marti","year":"2001","journal-title":"J Exp Med"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.2310\/JIM.0b013e3181b918fb","article-title":"P-Selectin and P-Selectin Glycoprotein Ligand 1 Mediate Rolling of Activated Cd8+ T Cells in Inflamed Colonic Venules","volume":"57","author":"Asaduzzaman","year":"2009","journal-title":"J Invest Med"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.3858\/emm.2009.41.10.079","article-title":"The Adaptor Protein Lad Tsad Mediates Laminin-Dependent T Cell Migration Via Association with the 67 Kda Laminin Binding Protein","volume":"41","author":"Park","year":"2009","journal-title":"Exp Mol Med"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1203877","article-title":"Focal Adhesion Kinase: A Regulator of Focal Adhesion Dynamics and Cell Movement","volume":"19","author":"Parsons","year":"2000","journal-title":"Oncogene"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M312848200","article-title":"Regulation of Cxc Chemokine Receptor 4-Mediated Migration by the Tec Family Tyrosine Kinase Itk","volume":"279","author":"Fischer","year":"2004","journal-title":"J Biol Chem"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.6.2927","article-title":"T Cell-Specific Adapter Protein Inhibits T Cell Activation by Modulating Lck Activity","volume":"165","author":"Sundvold","year":"2000","journal-title":"J Immunol"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-10-061838","article-title":"The Adaptor Protein Lad Associates with the G Protein Beta Subunit and Mediates Chemokine-Dependent T-Cell Migration","volume":"109","author":"Park","year":"2007","journal-title":"Blood"},{"key":"B36","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1371\/journal.pone.0009761","article-title":"T Cell Specific Adapter Protein (Tsad) Interacts with Tec Kinase Itk to Promote Cxcl12 Induced Migration of Human and Murine T Cells","volume":"5","author":"Berge","year":"2010","journal-title":"PloS One"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2007.11.005","article-title":"Expression of Sh2d2a in T-Cells Is Regulated Both at the Transcriptional and Translational Level","volume":"45","author":"Kolltveit","year":"2008","journal-title":"Mol Immunol"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.12.4494","article-title":"Attachment of the Psgl-1 Cytoplasmic Domain to the Actin Cytoskeleton Is Essential for Leukocyte Rolling on P-Selectin","volume":"99","author":"Snapp","year":"2002","journal-title":"Blood"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1158\/2326-6066.CIR-21-0690","article-title":"Targeting the Psgl-1 Immune Checkpoint Promotes Immunity to Pd-1-Resistant Melanoma","volume":"10","author":"DeRogatis","year":"2022","journal-title":"Cancer Immunol Res"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.869768","article-title":"Pd-1 Immune Checkpoint Blockade and Psgl-1 Inhibition Synergize to Reinvigorate Exhausted T Cells","volume":"13","author":"Viramontes","year":"2022","journal-title":"Front Immunol"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0902173","article-title":"P-Selectin Glycoprotein Ligand-1 Negatively Regulates T-Cell Immune Responses","volume":"183","author":"Matsumoto","year":"2009","journal-title":"J Immunol"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2017.02.002","article-title":"Psgl-1: A New Player in the Immune Checkpoint Landscape","volume":"38","author":"Tinoco","year":"2017","journal-title":"Trends Immunol"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.161.2.963","article-title":"Cd4+ T Cells Migrate into Inflamed Skin Only If They Express Ligands for E- and P-Selectin","volume":"161","author":"Tietz","year":"1998","journal-title":"J Immunol"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V86.9.3487.bloodjournal8693487","article-title":"Rolling in P-Selectin-Deficient Mice Is Reduced but Not Eliminated in the Dorsal Skin","volume":"86","author":"Yamada","year":"1995","journal-title":"Blood"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.178.4.2499","article-title":"Cd43 Collaborates with P-Selectin Glycoprotein Ligand-1 to Mediate E-Selectin-Dependent T Cell Migration into Inflamed Skin","volume":"178","author":"Matsumoto","year":"2007","journal-title":"J Immunol"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-10-3309","article-title":"Cd8+ T Cells from Patients with Acute Multiple Sclerosis Display Selective Increase of Adhesiveness in Brain Venules:A Critical Role for P-Selectin Glycoprotein Ligand-1","volume":"101","author":"Battistini","year":"2003","journal-title":"Blood"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2016.04.015","article-title":"Psgl-1 Is an Immune Checkpoint Regulator That Promotes T Cell Exhaustion","volume":"44","author":"Tinoco","year":"2016","journal-title":"Immunity"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1126\/science.adj6152","article-title":"Rapid Model-Guided Design of Organ-Scale Synthetic Vasculature for Biomanufacturing","volume":"388","author":"Sexton","year":"2025","journal-title":"Science"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1039\/d4bm00754a","article-title":"Construction of 3d Tumor in Vitro Models with an Immune Microenvironment Exhibiting Similar Tumor Properties and Biomimetic Physiological Functionality","volume":"13","author":"Jiang","year":"2024","journal-title":"Biomater Sci"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2786","article-title":"Non-Muscle Myosin Ii Takes Centre Stage in Cell Adhesion and Migration","volume":"10","author":"Vicente-Manzanares","year":"2009","journal-title":"Nat Rev Mol Cell Biol"},{"key":"B51","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1083\/jcb.201407059","article-title":"A Regulatory Motif in Nonmuscle Myosin Ii-B Regulates Its Role in Migratory Front-Back Polarity","volume":"209","author":"Juanes-Garcia","year":"2015","journal-title":"J Cell Biol"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.26117","article-title":"Expression of T-Cell Lymphoma Invasion and Metastasis 2 (Tiam2) Promotes Proliferation and Invasion of Liver Cancer","volume":"130","author":"Chen","year":"2012","journal-title":"Int J Cancer"},{"key":"B53","doi-asserted-by":"publisher","first-page":"2124","DOI":"10.1038\/s41467-018-04404-4","article-title":"Stef\/Tiam2-Mediated Rac1 Activity at the Nuclear Envelope Regulates the Perinuclear Actin Cap","volume":"9","author":"Woroniuk","year":"2018","journal-title":"Nat Commun"},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.7314\/apjcp.2013.14.11.6305","article-title":"Tiam2 Enhances Non-Small Cell Lung Cancer Cell Invasion and Motility","volume":"14","author":"Zhao","year":"2013","journal-title":"Asian Pac J Cancer Prev"},{"key":"B55","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.01.031","article-title":"Coronin 1b Coordinates Arp2\/3 Complex and Cofilin Activities at the Leading Edge","volume":"128","author":"Cai","year":"2007","journal-title":"Cell"},{"key":"B56","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00347-14","article-title":"Coronin1 Proteins Dictate Rac1 Intracellular Dynamics and Cytoskeletal Output","volume":"34","author":"Ojeda","year":"2014","journal-title":"Mol Cell Biol"},{"key":"B57","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.346114","article-title":"Novel Mechanism for Negatively Regulating Rho-Kinase (Rock) Signaling through Coronin1b Protein in Neuregulin 1 (Nrg-1)-Induced Tumor Cell Motility","volume":"287","author":"Rana","year":"2012","journal-title":"J Biol Chem"},{"key":"B58","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.178.9.5769","article-title":"Coronin Function Is Required for Chemotaxis and Phagocytosis in Human Neutrophils","volume":"178","author":"Yan","year":"2007","journal-title":"J Immunol"},{"key":"B59","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.1008666","article-title":"Peripheral Blood Cd4 T Lymphocytes from Multiple Sclerosis Patients Are Characterized by Higher Psgl-1 Expression and Transmigration Capacity across a Human Blood-Brain Barrier-Derived Endothelial Cell Line","volume":"86","author":"Bahbouhi","year":"2009","journal-title":"J Leucocyte Biol"},{"key":"B60","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2012.07.028","article-title":"Tyrosine Replacement of Psgl-1 Reduces Association Kinetics with P- and L-Selectin on the Cell Membrane","volume":"103","author":"Xiao","year":"2012","journal-title":"Biophys J"}],"container-title":["Frontiers in Immunology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fimmu.2025.1677361\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T09:54:49Z","timestamp":1764237289000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fimmu.2025.1677361\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,27]]},"references-count":60,"alternative-id":["10.3389\/fimmu.2025.1677361"],"URL":"https:\/\/doi.org\/10.3389\/fimmu.2025.1677361","relation":{},"ISSN":["1664-3224"],"issn-type":[{"value":"1664-3224","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,27]]},"article-number":"1677361"}}