{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T22:28:48Z","timestamp":1785364128004,"version":"3.55.0"},"reference-count":74,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T00:00:00Z","timestamp":1761696000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:p>\n                    GntR transcription factors are emerging as critical regulators of bacterial metabolism, stress responses, and pathogenicity, however, their roles in the virulence mechanisms of\n                    <jats:italic>Brucella abortus<\/jats:italic>\n                    remain poorly understood. In this study, we generated a\n                    <jats:italic>gntR8<\/jats:italic>\n                    (BAB_RS24500) deletion strain (\u0394\n                    <jats:italic>gntR8<\/jats:italic>\n                    ) in\n                    <jats:italic>B. abortus<\/jats:italic>\n                    2308 and systematically investigated its role in virulence. The results demonstrate that deletion of\n                    <jats:italic>gntR8<\/jats:italic>\n                    markedly impairs intracellular survival of\n                    <jats:italic>B. abortus<\/jats:italic>\n                    in RAW264.7 cells and significantly reduces virulence in a mouse infection model. Moreover, the \u0394\n                    <jats:italic>gntR8<\/jats:italic>\n                    strain exhibited increased sensitivity to oxidative stress, correlating with decreased expression of stress response genes. Integrative Dap-seq and RNA-seq analyses revealed that GntR8 directly binds to and positively regulates the\n                    <jats:italic>clpP<\/jats:italic>\n                    gene, a key component involved in oxidative stress defense. Deletion of\n                    <jats:italic>clpP<\/jats:italic>\n                    similarly resulted in diminished antioxidant capacity and intracellular survival, supporting a critical regulatory axis mediated by GntR8. Collectively, these findings provide novel insights into the molecular mechanisms by which GntR8 transcriptionally regulates oxidative stress responses and pathogenicity in\n                    <jats:italic>B. abortus<\/jats:italic>\n                    . The identification of GntR8 as a key virulence regulator highlights its potential as a therapeutic target, offering promising avenues for novel intervention strategies against brucellosis.\n                  <\/jats:p>","DOI":"10.3389\/fimmu.2025.1698057","type":"journal-article","created":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T06:45:32Z","timestamp":1761720332000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Deletion of the GntR8 transcriptional regulator impairs Brucella abortus intracellular survival and virulence by modulating stress response 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