{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T00:37:22Z","timestamp":1789173442825,"version":"build-2803163510"},"reference-count":54,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T00:00:00Z","timestamp":1770940800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006286","name":"Kongelig Hofbuntmager Aage Bangs Fond","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006286","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"\n Introduction<\/jats:title>\n Hidradenitis suppurativa is a painful inflammatory disease characterised by diverse cutaneous lesions that exhibit distinct clinical morphologies. The study aimed to examine the transcriptome of the various hidradenitis suppurativa lesion types.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n Transcriptomic analyses were performed using bulk RNA sequencing to examine the various lesion types, including draining tunnels, inflammatory nodules, non-draining tunnels, non-lesional skin from patients with hidradenitis suppurativa and normal skin from healthy individuals.<\/jats:p>\n <\/jats:sec>\n \n Results\/discussion<\/jats:title>\n \n Principal component and differential expression analyses revealed that these different lesion types exhibit distinct expression profiles while sharing common transcriptional features. Enrichment analysis of exclusively differentially expressed genes for each lesion type showed increased antigen presentation and cytotoxic T cell response in inflammatory nodules, including genes such as\n CD8B<\/jats:italic>\n ,\n HLA-C<\/jats:italic>\n , and\n HLA-F.<\/jats:italic>\n In accordance, cell-type enrichment analysis showed an elevated CD8\n +<\/jats:sup>\n T cell signature in inflammatory nodules. Gene set variation analysis demonstrated distinct inflammatory pathway signatures in hidradenitis suppurativa lesions, including heightened IL-1 signalling in draining tunnels with significant upregulation of\n IL1B<\/jats:italic>\n and its receptor\n IL1R<\/jats:italic>\n , but not\n IL1A.<\/jats:italic>\n Furthermore\n , IL17A<\/jats:italic>\n and\n IL17F<\/jats:italic>\n were significantly increased in draining tunnels, inflammatory nodules, and non-draining tunnels, with\n IL17F<\/jats:italic>\n showing the highest expression in draining tunnels.\n MMP3<\/jats:italic>\n ,\n MMP8<\/jats:italic>\n , and\n MMP10<\/jats:italic>\n were preferentially upregulated in draining tunnels, indicating a role in tunnel activity. Moreover,\n AQP4<\/jats:italic>\n and\n AQP5<\/jats:italic>\n were significantly downregulated in both lesional and non-lesional hidradenitis suppurativa skin, suggesting a possible involvement in preclinical pathogenic events.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n Distinct molecular signatures specific for each lesion type were identified, which may reflect differences in molecular mechanisms. These findings underscore the importance of distinguishing between lesion types in translational and clinical studies.<\/jats:p>\n <\/jats:sec>","DOI":"10.3389\/fimmu.2025.1715474","type":"journal-article","created":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T06:38:30Z","timestamp":1770964710000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Transcriptomic profiling reveals distinct molecular signatures among lesion types in hidradenitis suppurativa"],"prefix":"10.3389","volume":"16","author":[{"given":"Hakim","family":"Ben Abdallah","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Daugaard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louise","family":"Sch\u00f8sler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mads 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