{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T08:11:06Z","timestamp":1771920666411,"version":"3.50.1"},"reference-count":39,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T00:00:00Z","timestamp":1768348800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      N6-methyladenosine (m\n                      <jats:sup>6<\/jats:sup>\n                      A), the most prevalent and reversible post-transcriptional RNA modification, is involved in the progression of various diseases. Nonetheless, the role of m\n                      <jats:sup>6<\/jats:sup>\n                      A modification in Tuberculosis (TB) pathogenesis remains unknown. Here, we investigated the general expression patterns and potential functions of m\n                      <jats:sup>6<\/jats:sup>\n                      A regulators in TB.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The differentially expressed m\n                      <jats:sup>6<\/jats:sup>\n                      A genes between the healthy and TB groups were evaluated using the public Gene Expression Omnibus (GEO) database, and quantitative real-time PCR (qRT-PCR) was used to test the expression of key m\n                      <jats:sup>6<\/jats:sup>\n                      A regulators in our collected human TB and healthy samples. Random forest and LASSO regression analysis were performed to determine the prognostic performance of m\n                      <jats:sup>6<\/jats:sup>\n                      A regulators in TB patients. The relationship between m\n                      <jats:sup>6<\/jats:sup>\n                      A regulators and immune cells and immune reaction activity was analyzed through single-sample gene set enrichment analysis (ssGSEA). Unsupervised clustering was used to confirm that m\n                      <jats:sup>6<\/jats:sup>\n                      A regulators induced m\n                      <jats:sup>6<\/jats:sup>\n                      A modification patterns. The relationship between m\n                      <jats:sup>6<\/jats:sup>\n                      A modification patterns and the immune microenvironment, biological function, and TB subtype construction was evaluated by using Gene Set Enrichment Analysis (GSEA), Gene Ontology (GO) analysis and KEGG pathway analysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Our data revealed seven differentially expressed m\n                      <jats:sup>6<\/jats:sup>\n                      A -related genes-METTL3, VIRMA, YTHDF1, YTHDC1, YTHDC2, ELAVL1and LRPPRC mRNA-confirmed as critical m\n                      <jats:sup>6<\/jats:sup>\n                      A regulators in TB. The excellent diagnostic significance of these genes was further supported by the random forest, LASSO regression and clinical samples, which achieved a high area under the ROC (0.97). Unsupervised clustering classified patients into two m\n                      <jats:sup>6<\/jats:sup>\n                      A patterns with different immune microenvironment and biological feature.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Our study provides an overview of the expression patterns and potential roles of key m\n                      <jats:sup>6<\/jats:sup>\n                      A regulatory genes as diagnostic biomarkers and immunotherapy targets for TB, revealing their functions in TB pathogenesis. Our data may offer a valuable resource to guide both mechanistic and therapeutic analyses of key m\n                      <jats:sup>6<\/jats:sup>\n                      A regulators in TB.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fimmu.2025.1729362","type":"journal-article","created":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T05:16:12Z","timestamp":1768367772000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Expression and clinical value of key m6A RNA modification regulators in tuberculosis"],"prefix":"10.3389","volume":"16","author":[{"given":"Hongfei","family":"Du","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, The First Affiliated Hospital of Chengdu Medical College,School of Clinical Medicine,Chengdu Medical College","place":["Chengdu, Sichuan, 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College, Chengdu Seventh People\u2019s Hospital","place":["Chengdu, Sichuan, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The First Affiliated Hospital of Chengdu Medical College,School of Clinical Medicine,Chengdu Medical College","place":["Chengdu, Sichuan, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2026,1,14]]},"reference":[{"key":"B1","volume-title":"Global tuberculosis report 2020","year":"2020"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1111\/resp.13304","article-title":"Drug-resistant tuberculosis: An update on disease burden, diagnosis and treatment","volume":"23","author":"Lange","year":"2018","journal-title":"Respirology"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1405427","article-title":"Latent Mycobacterium tuberculosis 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