{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,5]],"date-time":"2026-07-05T05:13:54Z","timestamp":1783228434647,"version":"3.54.6"},"reference-count":129,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T00:00:00Z","timestamp":1776297600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:p>\n                    Leukocytes at the maternal-fetal interface have critical functions during pregnancy in the prevention of maternal reactivity towards fetal alloantigens, suppression of excess inflammation and promotion of placental angiogenesis. Failed maternal immune adaptations to pregnancy can lead to placental insufficiency and the development of severe reproductive complications. Recent evidence from mouse models has suggested that Nodal, a secreted morphogen of the TGF\u03b2 superfamily, is an immunoregulator of pregnancy. The absence of Nodal expression in the female reproductive tract (Nodal\n                    <jats:sup>\u0394\/\u0394<\/jats:sup>\n                    ) resulted in the loss of preimplantation regulatory T cells and a 50% implantation failure rate. By mid-gestation, Nodal-deficient pregnancies showed placental dysfunction, fetal loss and intrauterine growth restriction. Therefore, the Nodal\n                    <jats:sup>\u0394\/\u0394<\/jats:sup>\n                    model represents a unique system to study immune-mediated reproductive failure. In this study, single-cell RNA-sequencing was used to characterize leukocytes within the mid-gestational decidua and placenta during physiological and pathological Nodal\n                    <jats:sup>\u0394\/\u0394<\/jats:sup>\n                    pregnancies. Eleven distinct immune cell clusters were identified, with an emphasis on myeloid populations (macrophages and neutrophils). In particular, the transcriptional characterization of placenta-associated maternal macrophages revealed functions consistent with the regulation of angiogenesis, immune suppression and endocytosis. In Nodal\n                    <jats:sup>\u0394\/\u0394<\/jats:sup>\n                    females, the differential abundance and expression profile of these leukocytes reflected the immune dysfunction observed in human reproductive pathologies. Taken together, elucidating roles of leukocytes in placental development provides a basis in the understanding of mechanisms associated with physiological pregnancy and reproductive failures.\n                  <\/jats:p>","DOI":"10.3389\/fimmu.2026.1611813","type":"journal-article","created":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T14:29:41Z","timestamp":1776349781000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Single-cell RNA sequencing of leukocytes at the maternal-fetal interface in physiological and pathological Nodal-deficient pregnancies"],"prefix":"10.3389","volume":"17","author":[{"given":"Sarah","family":"Yull","sequence":"first","affiliation":[{"name":"Division of Experimental Medicine, McGill University","place":["Montr\u00e9al, QC, Canada"]},{"name":"Child Health and Human Development Program, Research Institute of the McGill University Health Centre","place":["Montr\u00e9al, QC, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain","family":"Pacis","sequence":"additional","affiliation":[{"name":"Canadian Centre for Computational Genomics, McGill University","place":["Montr\u00e9al, QC, Canada"]},{"name":"Victor Phillip Dahdaleh Institute of Genomic Medicine, McGill University","place":["Montr\u00e9al, QC, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Girardet","sequence":"additional","affiliation":[{"name":"Child Health and Human Development Program, Research Institute of the McGill University Health Centre","place":["Montr\u00e9al, QC, Canada"]},{"name":"Department of Obstetrics and Gynecology, McGill University","place":["Montr\u00e9al, QC, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Dufort","sequence":"additional","affiliation":[{"name":"Division of Experimental Medicine, McGill University","place":["Montr\u00e9al, QC, Canada"]},{"name":"Child Health and Human Development Program, Research Institute of the McGill University Health Centre","place":["Montr\u00e9al, QC, Canada"]},{"name":"Department of Obstetrics and Gynecology, McGill University","place":["Montr\u00e9al, QC, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,4,16]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1172\/jci122182","article-title":"Regulatory T cells in embryo implantation and the immune response to pregnancy","volume":"128","author":"Robertson","year":"2018","journal-title":"J Clin Invest"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00013.2018","article-title":"The female response to seminal fluid","volume":"100","author":"Schjenken","year":"2020","journal-title":"Physiol Rev"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1051\/rnd:19881006","article-title":"Immunoregulatory cells in human decidua: 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