{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T00:01:20Z","timestamp":1776297680582,"version":"3.50.1"},"reference-count":40,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T00:00:00Z","timestamp":1769990400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Abdominal aortic aneurysm (AAA) is a severe vascular disease that can lead to rupture and life-threatening hemorrhage. The role of ferroptosis in AAA pathogenesis remains insufficiently understood. This study aims to investigate the role of ferroptosis in AAA by identifying ferroptosis-associated molecular subtypes and examining their relationship with immunological characteristics using an artificial neural network (ANN) model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We analyzed three publicly available datasets (GSE7084, GSE47472, and GSE57691) to identify differentially expressed ferroptosis-related genes (FRGs) and employed consensus clustering to classify AAA samples into two subtypes. Immune infiltration was assessed with the CIBERSORT algorithm, and a diagnostic artificial neural network (ANN) model based on subtype-specific genes was developed to discriminate ferroptosis-associated molecular subtypes and derive the NeuraAAA score.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Nine differentially expressed FRGs were identified, and the model incorporated three key genes (oncostatin M, heme oxygenase-1, and interleukin-6), achieving high diagnostic accuracy (AUC\u00a0=\u00a00.988). Consensus clustering stratified AAA samples into two ferroptosis-associated subtypes with distinct immune profiles, with the C1 subtype showing higher immune infiltration and immune scores than C2. The derived NeuraAAA score was elevated in the immune-enriched subtype and correlated with immune-cell infiltration, and a nomogram integrating NeuraAAA and immune score showed good calibration. Immunofluorescence confirmed increased expression of all three genes in AAA specimens.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our study reveals the heterogeneous role of ferroptosis in AAA pathogenesis, demonstrating that ferroptosis-associated subtypes are linked to variations in the immune microenvironment. These findings provide new insights into AAA pathophysiology and suggest potential targets for subtype-specific therapeutic strategies, contributing to advances in precision medicine for AAA treatment.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fimmu.2026.1721069","type":"journal-article","created":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T08:46:56Z","timestamp":1770022016000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Artificial neural network-based analysis of ferroptosis-associated molecular subtypes and immunological profiles in abdominal aortic aneurysm"],"prefix":"10.3389","volume":"17","author":[{"given":"Maohua","family":"Li","sequence":"first","affiliation":[{"name":"Department of Vascular Surgery, The Second Xiangya Hospital of Central South University","place":["Changsha, China"]},{"name":"Molecular Biology Research Center, School of Life Sciences, Hunan Province Key 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University","place":["Changsha, China"]},{"name":"Institute of Vascular Diseases, Central South University","place":["Changsha, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lunchang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Vascular Surgery, The Second Xiangya Hospital of Central South University","place":["Changsha, China"]},{"name":"Institute of Vascular Diseases, Central South University","place":["Changsha, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Vascular Surgery, The Second Xiangya Hospital of Central South University","place":["Changsha, China"]},{"name":"Institute of Vascular Diseases, Central South University","place":["Changsha, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiehua","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Vascular Surgery, The 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