{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T15:07:04Z","timestamp":1783350424251,"version":"3.54.6"},"reference-count":33,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T00:00:00Z","timestamp":1770336000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014717","name":"National Outstanding Youth Science Fund Project of National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100014717","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Brucella<\/jats:italic>\n                      is a zoonotic pathogen capable of invading the host through the intestinal mucosa. However, the immune mechanisms underlying intestinal infection remain poorly understood. Tuft cells are specialized chemosensory epithelial cells in the intestine that can detect pathogen invasion and secrete IL-25, subsequently activating type 2 innate lymphoid cells (ILC2s) and playing a critical role in anti-parasitic immune responses. Nevertheless, whether the tuft cell-ILC2 circuit participates in immune responses against bacterial infections remains unclear. This study aimed to investigate the dynamic changes of tuft cells and ILC2s following\n                      <jats:italic>Brucella<\/jats:italic>\n                      infection, with a particular focus on elucidating the regulatory role of IL-25 in this process.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Thirty-six mice were divided into six groups: normal control (NC), isotype control (IC), infection groups at different time points (3, 7, and 14 days post-infection, designated as Inf-3d, Inf-7d, and Inf-14d, respectively), and an IL-25 blockade group (IL-25 Blk-7d, in which mice received anti-IL-25 neutralizing antibody treatment prior to\n                      <jats:italic>Brucella<\/jats:italic>\n                      infection and were analyzed at 7 days post-infection). In this study, immunofluorescence assay, flow cytometry, and Western blot were employed to detect the dynamic changes of tuft cells and ILC2s in intestinal tissues, as well as to determine the expression levels of pathway-related proteins.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The results showed that the numbers of tuft cells and ILC2s in the mouse intestine increased following\n                      <jats:italic>Brucella<\/jats:italic>\n                      infection, peaking at 7 days post-infection. Pretreatment with IL-25 neutralizing antibody significantly suppressed the proliferation of these two cell populations. Western blot analysis further confirmed that the expression levels of tuft cell-associated proteins (PO2F3, DCLK1, and IL-25) and ILC2-associated proteins (GATA3 and IL-13) were upregulated in infected intestinal tissues, whereas IL-25 blockade treatment inhibited the expression of these proteins. Correlation analysis revealed a remarkably strong positive correlation between the proportions of tuft cells and ILC2s, and this correlation was completely abolished following IL-25 neutralization.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>These findings confirm that the activation of the tuft cell-ILC2 circuit is dependent on the regulatory role of IL-25. These findings extend the antimicrobial role of the tuft cell-ILC2 circuit beyond parasitic immunity and identify IL-25 as an essential regulatory mediator in antibacterial defense at the intestinal mucosa.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fimmu.2026.1732274","type":"journal-article","created":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T06:55:55Z","timestamp":1770360955000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Tuft-cell-derived IL-25 regulates intestinal ILC2 in response to Brucella infection"],"prefix":"10.3389","volume":"17","author":[{"given":"Kaiyu","family":"Shang","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, The First Affiliated Hospital of Xinjiang Medical University","place":["Urumqi, Xinjiang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The First Affiliated Hospital of Xinjiang Medical University","place":["Urumqi, Xinjiang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The First Affiliated Hospital of Xinjiang Medical University","place":["Urumqi, Xinjiang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huidong","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The First Affiliated Hospital of Xinjiang Medical University","place":["Urumqi, Xinjiang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The First Affiliated Hospital of Xinjiang Medical University","place":["Urumqi, Xinjiang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinxin","family":"Qi","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The First Affiliated Hospital of Xinjiang Medical University","place":["Urumqi, Xinjiang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuejie","family":"Zhu","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Center, The First Affiliated Hospital of Xinjiang Medical University","place":["Urumqi, Xinjiang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengbo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The First Affiliated Hospital of Xinjiang Medical University","place":["Urumqi, Xinjiang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,2,6]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(06)70382-6","article-title":"The new global map of human brucellosis","volume":"6","author":"Pappas","year":"2006","journal-title":"Lancet Infect Dis"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1080\/07853890.2023.2295398","article-title":"Brucellosis: epidemiology, pathogenesis, diagnosis and treatment\u2013a comprehensive review","volume":"55","author":"Qureshi","year":"2023","journal-title":"Ann Med"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.3201\/eid2909.230052","article-title":"Global estimate of human brucellosis incidence","volume":"29","author":"Laine","year":"2023","journal-title":"Emerg Infect Dis J"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.01436","article-title":"Omp19 enables brucella abortus to evade the antimicrobial activity from host\u2019s proteolytic defense system","volume":"10","author":"Pasquevich","year":"2019","journal-title":"Front Immunol"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.15-0246","article-title":"Outbreak of human brucellosis from consumption of raw goats\u2019 Milk in penang","volume":"93","author":"Leong","year":"2015","journal-title":"Malaysia. 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