{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T06:49:33Z","timestamp":1776840573464,"version":"3.51.2"},"reference-count":40,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T00:00:00Z","timestamp":1776816000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>YKT6 is markedly overexpressed across multiple tumor types and plays a role in driving their progression. However, the correlation between YKT6 and breast cancer remains poorly understood. Therefore, we aimed to investigate the potential prognostic value and biological function of YKT6 gene in breast cancer.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Public datasets, clinic sample and tissue microarray (TMA) were used for YKT6 expression and prognostic value analyses. The relevance between YKT6 expression, tumor-immune infiltrates and tumor mutation burden (TMB) was examined using the TCGA database. Cellular functional assays were performed to verify the biological behavior of YKT6 in breast cancer cells. Moreover, transcriptome sequencing (RNA-seq) was conducted to explore the underlying mechanism of YKT6.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>YKT6 was significantly upregulated in breast cancer tissue comparing to normal tissue(P&amp;lt;0.05) and higher YKT6 expression was significantly linked to worse clinical prognosis, advanced tumor stages, and distant metastasis(P&amp;lt;0.05). Additionally, YKT6 expression is correlated with the infiltration of various immune cell and TMB. Knockdown of YKT6 impaired the proliferation, invasion, and migration abilities of breast cancer cells, and increased apoptosis. Functional enrichment analysis revealed that YKT6 primarily influenced breast cancer progression through the cell cycle, as well as biological processes such as autophagy, apoptosis, and ferroptosis. Moreover, knockdown of YKT6 suppressed the activity of mTORC1.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>YKT6 may serve as a potential prognostic biomarker for breast cancer. The expression level of YKT6 was correlated with tumor-infiltrating immune cells in breast cancer. It may offer potential value for the treatment of breast cancer patients.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fimmu.2026.1742661","type":"journal-article","created":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T05:51:05Z","timestamp":1776837065000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["YKT6 promotes breast cancer progression and is associated with poor prognosis and immune infiltration"],"prefix":"10.3389","volume":"17","author":[{"given":"Meilin","family":"Zhang","sequence":"first","affiliation":[{"name":"Obstetrics & Gynecology Hospital of Fudan University, Shanghai Key Lab of Reproduction and Development, Shanghai Key Lab of Female Reproductive Endocrine Related Diseases","place":["Shanghai, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingjing","family":"Yuan","sequence":"additional","affiliation":[{"name":"Obstetrics & Gynecology Hospital of Fudan University, Shanghai Key Lab of Reproduction and Development, Shanghai Key Lab of Female Reproductive Endocrine Related Diseases","place":["Shanghai, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaxuan","family":"Liu","sequence":"additional","affiliation":[{"name":"Obstetrics & Gynecology Hospital of Fudan University, Shanghai Key Lab of Reproduction and Development, Shanghai Key Lab of Female Reproductive Endocrine Related Diseases","place":["Shanghai, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiran","family":"Qiu","sequence":"additional","affiliation":[{"name":"Obstetrics & Gynecology Hospital of Fudan University, Shanghai Key Lab of Reproduction and Development, Shanghai Key Lab of Female Reproductive Endocrine Related Diseases","place":["Shanghai, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingdi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Obstetrics & Gynecology Hospital of Fudan University, Shanghai Key Lab of Reproduction and Development, Shanghai Key Lab of Female Reproductive Endocrine Related Diseases","place":["Shanghai, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongliang","family":"Chen","sequence":"additional","affiliation":[{"name":"Obstetrics & Gynecology Hospital of Fudan University, Shanghai Key Lab of Reproduction and Development, Shanghai Key Lab of Female Reproductive Endocrine Related Diseases","place":["Shanghai, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2026,4,22]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21834","article-title":"Global cancer statistics 2022: Globocan estimates of incidence and mortality worldwide for 36 cancers in 185 countries","volume":"74","author":"Bray","year":"2024","journal-title":"CA Cancer J Clin"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.21147\/j.issn.1000-9604.2025.06.04","article-title":"Cancer statistics in China: Epidemiology, risk factors, and prevention","volume":"37","author":"Cao","year":"2025","journal-title":"Chin J Cancer Res"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.21147\/j.issn.1000-9604.2025.04.10","article-title":"Association of lifestyle factors with breast cancer incidence: An overview of systematic reviews","volume":"37","author":"Rana","year":"2025","journal-title":"Chin J Cancer Res"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21863","article-title":"Breast cancer statistics 2024","volume":"74","author":"Giaquinto","year":"2024","journal-title":"CA Cancer J Clin"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-023-00668-x","article-title":"Mechanisms of snare proteins in membrane fusion","volume":"25","author":"Jahn","year":"2024","journal-title":"Nat Rev Mol Cell Biol"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1006698","article-title":"Vamp3\/Syb and Ykt6 are required for the fusion of constitutive secretory carriers with the plasma membrane","volume":"13","author":"Gordon","year":"2017","journal-title":"PLoS Genet"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2350-23.2024","article-title":"Alpha-synuclein inhibits the secretion of extracellular vesicles through disruptions in Ykt6 lipidation","volume":"45","author":"Tsunemi","year":"2025","journal-title":"J Neurosci"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.18632\/aging.103268","article-title":"Lncrna Pvt1 promotes exosome secretion through Ykt6, Rab7, and Vamp3 in pancreatic cancer","volume":"12","author":"Sun","year":"2020","journal-title":"Aging (Albany NY)"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1007\/s12079-017-0421-7","article-title":"Pathways of production and delivery of hepatocyte exosomes","volume":"12","author":"Chen","year":"2018","journal-title":"J Cell Commun Signal"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201712058","article-title":"Autophagosomal Ykt6 is required for fusion with lysosomes independently of Syntaxin 17","volume":"217","author":"Matsui","year":"2018","journal-title":"J Cell Biol"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-021-00392-4","article-title":"Machinery, regulation and pathophysiological implications of autophagosome maturation","volume":"22","author":"Zhao","year":"2021","journal-title":"Nat Rev Mol Cell Biol"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2021.01.001","article-title":"The biology, function, and applications of exosomes in cancer","volume":"11","author":"Liu","year":"2021","journal-title":"Acta Pharm Sin B"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2018.01.005","article-title":"Snare complex assembly and disassembly","volume":"28","author":"Yoon","year":"2018","journal-title":"Curr Biol"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3700044","article-title":"Cdna microarray analysis of invasive and tumorigenic phenotypes in a breast cancer model","volume":"84","author":"Kluger","year":"2004","journal-title":"Lab Invest"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1126-2","article-title":"Llgl2 rescues nutrient stress by promoting leucine uptake in Er(+) breast cancer","volume":"569","author":"Saito","year":"2019","journal-title":"Nature"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1080\/2162402X.2021.1938890","article-title":"Ykt6, as a potential predictor of prognosis and immunotherapy response for oral squamous cell carcinoma, is related to cell invasion, metastasis, and Cd8+ T cell infiltration","volume":"10","author":"Yang","year":"2021","journal-title":"Oncoimmunology"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2020.108351","article-title":"Knockdown of Tbrg4 suppresses proliferation, invasion and promotes apoptosis of osteosarcoma cells by downregulating Tgf-beta1 expression and Pi3k\/Akt signaling pathway","volume":"686","author":"Huang","year":"2020","journal-title":"Arch Biochem Biophys"},{"key":"B18","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1016\/j.ejca.2017.06.003","article-title":"New liver cancer biomarkers: Pi3k\/Akt\/Mtor pathway members and eukaryotic translation initiation factors","volume":"83","author":"Golob-Schwarzl","year":"2017","journal-title":"Eur J Cancer"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiae573","article-title":"Mir-124-3p\/Eif3b regulates host cell apoptosis induced by Chlamydia psittaci through Pi3k\/Akt signaling pathway","volume":"231","author":"Tong","year":"2025","journal-title":"J Infect Dis"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2020.1823124","article-title":"New insights regarding snare proteins in autophagosome-lysosome fusion","volume":"17","author":"Tian","year":"2021","journal-title":"Autophagy"},{"key":"B21","doi-asserted-by":"publisher","first-page":"1799","DOI":"10.3390\/biom12121779","article-title":"Snare modulators and snare mimetic peptides","volume":"12","author":"Khvotchev","year":"2022","journal-title":"Biomolecules"},{"key":"B22","doi-asserted-by":"publisher","first-page":"189148","DOI":"10.1016\/j.bbcan.2024.189148","article-title":"Snare proteins: Core engines of membrane fusion in cancer","author":"Liu","year":"2024","journal-title":"Biochim Biophys Acta Rev Cancer"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2021.679955","article-title":"Targeting snare-mediated vesicle transport to block invadopodium-based cancer cell invasion","volume":"11","author":"Gorshtein","year":"2021","journal-title":"Front Oncol"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2018.01.031","article-title":"Stx3 represses the stability of the tumor suppressor Pten to activate the Pi3k-Akt-Mtor signaling and promotes the growth of breast cancer cells","volume":"1864","author":"Nan","year":"2018","journal-title":"Biochim Biophys Acta Mol Basis Dis"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.86636","article-title":"Syntaxin-6 promotes the progression of hepatocellular carcinoma and alters its sensitivity to chemotherapies by activating the Usf2\/Lc3b axis","volume":"19","author":"Zhou","year":"2023","journal-title":"Int J Biol Sci"},{"key":"B26","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.bbcan.2015.04.002","article-title":"Role of snare proteins in tumourigenesis and their potential as targets for novel anti-cancer therapeutics","volume":"1856","author":"Meng","year":"2015","journal-title":"Biochim Biophys Acta"},{"key":"B27","doi-asserted-by":"publisher","first-page":"1235","DOI":"10.1186\/s12885-024-12975-3","article-title":"Comprehensive analysis identifies Ykt6 as a potential prognostic and diagnostic biomarker in lung adenocarcinoma","volume":"24","author":"Zhang","year":"2024","journal-title":"BMC Cancer"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1080\/00365521.2021.1961305","article-title":"High expression of Ykt6 associated with progression and poor prognosis of hepatocellular carcinoma","volume":"56","author":"Xu","year":"2021","journal-title":"Scand J Gastroenterol"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000033546","article-title":"A pan-cancer analysis of the oncogenic role of Ykt6 in human tumors","volume":"102","author":"Zhang","year":"2023","journal-title":"Med (Baltimore)"},{"key":"B30","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1016\/j.cancergen.2025.06.004","article-title":"Assessing germline mutational profile and its clinicopathological associations in triple negative breast cancer","author":"John","year":"2025","journal-title":"Cancer Genet"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1016\/j.cancergen.2024.06.001","article-title":"Half of most frequently mutated genes in breast cancer are expressed differentially between premenopausal and postmenopausal breast cancer patients","author":"Berkel","year":"2024","journal-title":"Cancer Genet"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.3389\/pore.2025.1611983","article-title":"Tumour budding in invasive ductal breast carcinomas: Correlation with clinicopathological prognostic parameters and hormone receptor status","volume":"31","author":"Francis","year":"2025","journal-title":"Pathol Oncol Res"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2021.09.002","article-title":"Tumor-derived exosomes drive immunosuppressive macrophages in a pre-metastatic niche through glycolytic dominant metabolic reprogramming","volume":"33","author":"Morrissey","year":"2021","journal-title":"Cell Metab"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1002\/wsbm.70004","article-title":"Anna Karenina principle, immune-oncology-microbiome trio and cancer microbiome therapy","volume":"17","author":"Ma","year":"2025","journal-title":"WIREs Mech Dis"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.14336\/AD.2025.1265","article-title":"Neoadjuvant immunotherapy in triple-negative breast cancer: Striking potential or need for caution","author":"Li","year":"2025","journal-title":"Aging Dis"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.14336\/AD.2023.1002","article-title":"Geriatric breast cancer: Staging, molecular surrogates, and treatment. A review & meta-analysis","volume":"15","author":"Fonseca","year":"2024","journal-title":"Aging Dis"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.34133\/research.0915","article-title":"Gene signature-based drug screening reveals ponatinib enhances immunotherapy efficacy in triple-negative breast cancer by reversing Mdsc-mediated immunosuppressive tumor microenvironment","volume":"8","author":"Wang","year":"2025","journal-title":"Res (Wash D C)"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1016\/j.xcrm.2024.101686","article-title":"Soluble Tim-3 serves as a tumor prognostic marker and therapeutic target for Cd8(+) T cell exhaustion and anti-Pd-1 resistance","volume":"5","author":"Chen","year":"2024","journal-title":"Cell Rep Med"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1007\/s10549-006-9293-x","article-title":"Possible involvement of Cct5, Rgs3, and Ykt6 genes up-regulated in P53-mutated tumors in resistance to docetaxel in human breast cancers","volume":"101","author":"Ooe","year":"2007","journal-title":"Breast Cancer Res Treat"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.3389\/pore.2025.1612179","article-title":"Modelling metastasis in a dish: In vitro approaches for studying breast cancer progression","volume":"31","author":"Duman","year":"2025","journal-title":"Pathol Oncol Res"}],"container-title":["Frontiers in Immunology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fimmu.2026.1742661\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T05:51:05Z","timestamp":1776837065000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fimmu.2026.1742661\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,22]]},"references-count":40,"alternative-id":["10.3389\/fimmu.2026.1742661"],"URL":"https:\/\/doi.org\/10.3389\/fimmu.2026.1742661","relation":{},"ISSN":["1664-3224"],"issn-type":[{"value":"1664-3224","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,22]]},"article-number":"1742661"}}