{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T16:52:23Z","timestamp":1786121543056,"version":"build-2736575974"},"reference-count":76,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T00:00:00Z","timestamp":1786060800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      This study investigates, for the first time, the effects of plasma-treated alginate hydrogels (PTH) on porcine leukocyte activity\n                      <jats:italic>in vitro<\/jats:italic>\n                      . Cold atmospheric plasma (CAP) generates reactive oxygen and nitrogen species (RONS), which can modulate immune cell behavior and may promote tissue repair.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Our findings demonstrate that PTH induce a controlled increase in nitrite and superoxide production in both neutrophil and monocyte cultures, particularly at higher treatment levels, without evidence of lytic cell death or significant loss of viability. PTH application resulted in a modest non-significant decrease in neutrophil metabolic activity without promoting degranulation, while monocyte metabolism remained unaffected. These results suggest that therapeutic doses of CAP elicit moderate oxidative stress capable of activating leukocytes while preserving their functional stability.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Overall, PTH demonstrated low cytotoxicity toward host immune cells\n                      <jats:italic>in vitro<\/jats:italic>\n                      , suggesting they may be eventually employed as bioactive materials for supporting early inflammatory responses. While their potential to enhance wound healing or tissue repair remains speculative, our findings warrant further investigation for applications in both human and veterinary medicine.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fimmu.2026.1750122","type":"journal-article","created":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T05:28:08Z","timestamp":1786080488000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Plasma-treated alginate hydrogels modulate porcine leukocyte activity in vitro"],"prefix":"10.3389","volume":"17","author":[{"given":"Joanna","family":"Wessely-Szponder","sequence":"first","affiliation":[{"name":"Department of Pathophysiology, Faculty of Veterinary Medicine, University of Life Sciences","place":["Lublin, Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulia","family":"Fornabaio","sequence":"additional","affiliation":[{"name":"Biomaterials, Biomechanics and Tissue Engineering (BBT) Group, Department of Materials Science and Engineering and Institute for Research and Innovation in Health (IRIS), Universitat Polit\u00e8cnica de Catalunya - BarcelonaTech (UPC)","place":["Barcelona, Spain"]},{"name":"Barcelona Research Centre in Multiscale Science and Engineering (CCEM), UPC","place":["Barcelona, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominika","family":"Nguyen Ngoc","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology, Faculty of Veterinary Medicine, University of Life Sciences","place":["Lublin, Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beata","family":"Drzewiecka","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology, Faculty of Veterinary Medicine, University of Life Sciences","place":["Lublin, Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Canal","sequence":"additional","affiliation":[{"name":"Biomaterials, Biomechanics and Tissue Engineering (BBT) Group, Department of Materials Science and Engineering and Institute for Research and Innovation in Health (IRIS), Universitat Polit\u00e8cnica de Catalunya - BarcelonaTech (UPC)","place":["Barcelona, Spain"]},{"name":"Barcelona Research Centre in Multiscale Science and Engineering (CCEM), UPC","place":["Barcelona, Spain"]},{"name":"CIBER de Bioingenier\u00eda, Biomateriales y Nanomedicina (CIBER-BBN), Instituto de Salud Carlos III","place":["Madrid, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,8,7]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-017-2785-7","article-title":"Neutrophils in tissue injury and repair","volume":"371","author":"Wang","year":"2018","journal-title":"Cell Tissue Res"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.3390\/cells11244076","article-title":"Anti-inflammatory neutrophil functions in the resolution of inflammation and tissue repair","volume":"11","author":"Loh","year":"2022","journal-title":"Cells"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines13030694","article-title":"Neutrophil heterogeneity in wound healing","volume":"13","author":"Ren\u00f2","year":"2025","journal-title":"Biomedicines"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2006.09.030","article-title":"Reactive oxygen and nitrogen species as signaling molecules regulating neutrophil function","volume":"42","author":"Fialkow","year":"2007","journal-title":"Free Radical Biol Med"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1177\/1177271918755391","article-title":"Reactive oxygen species: A key constituent in cancer survival","volume":"13","author":"Kumari","year":"2018","journal-title":"biomark Insights"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1111\/wrr.12364","article-title":"Dual effects of atmospheric pressure plasma jet on skin wound healing of mice","volume":"23","author":"Xu","year":"2015","journal-title":"Wound Repair Regener"},{"key":"B7","doi-asserted-by":"publisher","first-page":"5621","DOI":"10.1038\/s41598-018-24049-z","article-title":"In vivo study of non-invasive effects of non-thermal plasma in pressure ulcer treatment","volume":"8","author":"Chatraie","year":"2018","journal-title":"Sci Rep"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/2318680","article-title":"Regulation of redox homeostasis by nonthermal biocompatible plasma discharge in stem cell differentiation","volume":"2019","author":"Li","year":"2019","journal-title":"Oxid Med Cell Longevity"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202205803","article-title":"Current state of cold atmospheric plasma and cancer-immunity cycle: Therapeutic relevance and overcoming clinical limitations using hydrogels","volume":"10","author":"\u017divani\u0107","year":"2023","journal-title":"Adv Sci"},{"key":"B10","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1186\/s12964-023-01398-5","article-title":"Interplay of oxidative stress, cellular communication and signaling pathways in cancer","volume":"22","author":"Iqbal","year":"2024","journal-title":"Cell Commun Signaling"},{"key":"B11","doi-asserted-by":"publisher","first-page":"1534","DOI":"10.1038\/s42003-024-07219-w","article-title":"Cellular and molecular roles of reactive oxygen species in wound healing","volume":"7","author":"Hunt","year":"2024","journal-title":"Commun Biol"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2022.114004","article-title":"The initiation of oxidative stress and therapeutic strategies in wound healing","volume":"157","author":"Wang","year":"2023","journal-title":"Biomedicine Pharmacotherapy"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.3389\/fmats.2021.681960","article-title":"This review recent advances in chitosan and alginate\u2010based hydrogels for wound healing application","volume":"8","author":"Kibungu","year":"2021","journal-title":"Front Mater"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1080\/00914037.2022.2075872","article-title":"A review on hydrogels classification and recent developments in biomedical applications","volume":"72","author":"Kasai","year":"2023","journal-title":"Int J Polym Mater Polym Biomater"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1186\/s13036-020-0227-7","article-title":"Alginate-based hydrogels as drug delivery vehicles in cancer treatment and their applications in wound dressing and 3D bioprinting","volume":"14","author":"Abasalizadeh","year":"2020","journal-title":"J Biol Eng"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2020.00665","article-title":"Modulating alginate hydrogels for improved biological performance as cellular 3D microenvironments","volume":"8","author":"Neves","year":"2020","journal-title":"Front Bioeng Biotechnol"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2024.135019","article-title":"Alginate-based hydrogels mediated biomedical applications: A review","volume":"279","author":"Ren","year":"2024","journal-title":"Int J Biol Macromol"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2024.07.013","article-title":"Healing wounds with plasma-activated hydrogel therapy","volume":"43","author":"Sabrin","year":"2025","journal-title":"Trends Biotechnol"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2023.128841","article-title":"Engineering alginate-based injectable hydrogels combined with bioactive polymers for targeted plasma-derived oxidative stress delivery in osteosarcoma therapy","volume":"257","author":"Espona-Noguera","year":"2024","journal-title":"Int J Biol Macromol"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines12092122","article-title":"Exploring the antibacterial and regenerative properties of a two-stage alginate wound dressing in a rat model of purulent wounds","volume":"12","author":"Atepileva","year":"2024","journal-title":"Biomedicines"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.3390\/jpm11090890","article-title":"Current trends in advanced alginate-based wound dressings for chronic wounds","volume":"11","author":"Barbu","year":"2021","journal-title":"J Personalized Med"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1109\/TRPMS.2024.3486975","article-title":"Demonstration of the high efficiency of an air plasma jet combining electric field and RONS in the treatment of chronic wounds","volume":"9","author":"Cort\u00e1zar","year":"2025","journal-title":"IEEE Trans Radiat Plasma Med Sci"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1097\/GOX.0000000000007553","article-title":"Jet cold plasma at atmospheric air pressure for venous ulcers: A randomized clinical trial","volume":"14","author":"Henares","year":"2026","journal-title":"Plast Reconstr Surg Glob Open"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.12968\/jowc.2025.0207","article-title":"Treatment of chronic wounds with cold plasma: A randomised, single-blind, placebo-controlled clinical study","volume":"34","author":"Strohal","year":"2025","journal-title":"J Wound Care"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1001\/jamanetworkopen.2020.10411","article-title":"Effect of cold atmospheric plasma therapy vs standard therapy placebo on wound healing in patients with diabetic foot ulcers: A randomized clinical trial","volume":"3","author":"Stratmann","year":"2020","journal-title":"JAMA Netw Open"},{"key":"B26","doi-asserted-by":"publisher","first-page":"3645","DOI":"10.1038\/s41598-022-07333-x","article-title":"Chronic wounds treated with cold atmospheric plasmajet versus best practice wound dressings: A multicenter, randomized, non-inferiority trial","volume":"12","author":"Strohal","year":"2022","journal-title":"Sci Rep"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.3390\/jcm14217533","article-title":"Intraoperative application of cold atmospheric plasma reduces inguinal wound healing disorders\u2014a pilot study","volume":"14","author":"Werra","year":"2025","journal-title":"J Clin Med"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.12490","article-title":"Alleviation of chronic venous leg ulcers with a hand-held dielectric barrier discharge plasma generator (PlasmaDerm(\u00ae) VU-2010): Results of a monocentric, two-armed, open, prospective, randomized and controlled trial (NCT01415622)","volume":"29","author":"Brehmer","year":"2015","journal-title":"J Eur Acad Dermatol Venereol"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.3390\/ani15070968","article-title":"Cold atmospheric plasma in oncology: A review and perspectives on its application in veterinary oncology","volume":"15","author":"Holanda","year":"2025","journal-title":"Animals"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.4142\/jvs.23067","article-title":"Application of cold atmospheric microwave plasma as an adjunct therapy for wound healing in dogs and cats","volume":"24","author":"Yoo","year":"2023","journal-title":"J Vet Sci"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcam.2023.100773","article-title":"Use of cold atmospheric plasma in the treatment of squamous cell carcinoma: in vitro effects and clinical application in feline tumors: A pilot study","author":"Holanda","year":"2023","journal-title":"Topics Companion Anim Med"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1002\/adtp.202300399","article-title":"Cold atmospheric plasma: An emerging immunomodulatory therapy","volume":"7","author":"Liu","year":"2024","journal-title":"Adv Ther"},{"key":"B33","doi-asserted-by":"publisher","first-page":"16160","DOI":"10.1038\/s41598-019-52673-w","article-title":"Production of reactive species in alginate hydrogels for cold atmospheric plasma-based therapies","volume":"9","author":"Labay","year":"2019","journal-title":"Sci Rep"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1039\/D2BM00308B","article-title":"Thermosensitive hydrogels to deliver reactive species generated by cold atmospheric plasma: A case study with methylcellulose","volume":"10","author":"Sole-Mart\u00ed","year":"2022","journal-title":"Biomater Sci"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1021\/acs.molpharmaceut.4c00595","article-title":"Hydrogels as a potential biomaterial for multimodal therapeutic applications","volume":"21","author":"Kaur","year":"2024","journal-title":"Mol Pharmaceutics"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.3390\/jcs8110457","article-title":"Comprehensive review of hydrogel synthesis, characterization, and emerging applications","volume":"8","author":"Priya","year":"2024","journal-title":"J Compos Sci"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25073849","article-title":"Advancements in regenerative hydrogels in skin wound treatment: A comprehensive review","volume":"25","author":"Olteanu","year":"2024","journal-title":"Int J Mol Sci"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202102599","article-title":"Flexible bicolorimetric polyacrylamide\/chitosan hydrogels for smart real-time monitoring and promotion of wound healing","volume":"31","author":"Zheng","year":"2021","journal-title":"Adv Funct Mater"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202401490","article-title":"Chitosan-based hydrogels as antibacterial\/antioxidant\/anti-inflammation multifunctional dressings for chronic wound healing","volume":"13","author":"Xu","year":"2024","journal-title":"Adv Healthcare Mater"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.5c12685","article-title":"Multifunctional plasma-activated alginate dressing potentiates the moist healing of chronic infected wounds","volume":"17","author":"Jin","year":"2025","journal-title":"ACS Appl Mater Interfaces"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.3A0415-165RR","article-title":"Neutrophil extracellular trap formation is elicited in response to cold physical plasma","volume":"100","author":"Bekeschus","year":"2016","journal-title":"J Leukocyte Biol"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.3390\/biom11060902","article-title":"Cold atmospheric plasma promotes the immunoreactivity of granulocytes in vitro","volume":"11","author":"Kupke","year":"2021","journal-title":"Biomolecules"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.3791\/65301","article-title":"Swine model of biofilm infection and invisible wounds","author":"Masry","year":"2023","journal-title":"J Visualized Experiments (JoVE)"},{"key":"B44","doi-asserted-by":"publisher","first-page":"66","DOI":"10.1046\/j.1524-475x.2001.00066.x","article-title":"The pig as a model for human wound healing","volume":"9","author":"Sullivan","year":"2001","journal-title":"Wound Repair Regener"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202312005","article-title":"Injectable plasma-treated alginate hydrogel for oxidative stress delivery to induce immunogenic cell death in osteosarcoma","volume":"34","author":"\u017divani\u0107","year":"2024","journal-title":"Adv Funct Mater"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1038\/s41596-021-00521-5","article-title":"Evaluation of the effects of cold atmospheric plasma and plasma-treated liquids in cancer cell cultures","volume":"16","author":"Tornin","year":"2021","journal-title":"Nat Protoc"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.0c04906","article-title":"Quantification of plasma-produced hydroxyl radicals in solution and their dependence on the pH","volume":"93","author":"Tampieri","year":"2021","journal-title":"Anal Chem"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2017.02.033","article-title":"Quantitative determination of trace hydrogen peroxide in the presence of sulfide using the Amplex Red\/horseradish peroxidase assay","volume":"963","author":"Wang","year":"2017","journal-title":"Anal Chim Acta"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1002\/ppap.202100062","article-title":"Validation of colorimetric assays for hydrogen peroxide, nitrate and nitrite ions in complex plasma-treated water solutions","volume":"18","author":"Veronico","year":"2021","journal-title":"Plasma Processes Polym"},{"key":"B50","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1016\/j.jcpa.2019.12.007","article-title":"The role of antimicrobial neutrophil extract in modification of the inflammatory response during osteochondral autograft and allograft transplantation in rabbits","volume":"175","author":"Wessely-Szponder","year":"2020","journal-title":"J Comp Pathol"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.3390\/ma16165691","article-title":"Analysis of In Vitro leukocyte responses to biomaterials in the presence of antimicrobial porcine neutrophil extract (AMPNE)","volume":"16","author":"Drzewiecka","year":"2023","journal-title":"Materials"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.33562","article-title":"Suppression of \u03b2-catenin signaling in colon carcinoma cells by a bacterial protein","volume":"149","author":"Nadeem","year":"2021","journal-title":"Int J Cancer"},{"key":"B53","doi-asserted-by":"publisher","first-page":"68","DOI":"10.1016\/j.jim.2017.06.012","article-title":"Optimized incubation regime for nitric oxide measurements in murine macrophages using the Griess assay","volume":"449","author":"Schm\u00f6lz","year":"2017","journal-title":"J Immunol Methods"},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.31138\/mjr.34.2.163","article-title":"Evaluation of NADPH oxidase (NOX) activity by nitro blue tetrazolium (NBT) test in SLE patients","volume":"34","author":"Baisya","year":"2023","journal-title":"Mediterr J Rheumatol"},{"key":"B55","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.816037","article-title":"Neutrophils culture in collagen gel system","volume":"13","author":"Li","year":"2022","journal-title":"Front Immunol"},{"key":"B56","doi-asserted-by":"publisher","DOI":"10.5395\/rde.2014.39.3.149","article-title":"In vitro cytotoxicity of four calcium silicate-based endodontic cements on human monocytes, a colorimetric MTT assay","volume":"39","author":"Khedmat","year":"2014","journal-title":"Restor Dent Endod"},{"key":"B57","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot095497","article-title":"Analysis of cell viability by the lactate dehydrogenase assay","volume":"2018","author":"Kumar","year":"2018","journal-title":"Cold Spring Harb Protoc"},{"key":"B58","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.2c01391","article-title":"Potentials of a plasma-aerosol system for wound healing advanced by drug introduction: An In Vitro study","volume":"9","author":"Srema\u010dki","year":"2023","journal-title":"ACS Biomater Sci Eng"},{"key":"B59","doi-asserted-by":"publisher","DOI":"10.1016\/j.jddst.2024.105532","article-title":"Hybrid nanocomposite wound dressings by a novel nanorod vitamin-B3-Ag metal-organic framework and bacterial cellulose nanofibers","volume":"95","author":"Barjasteh","year":"2024","journal-title":"J Drug Delivery Sci Technol"},{"key":"B60","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2023.101849","article-title":"Regulated cell death in neutrophils: From apoptosis to NETosis and pyroptosis","volume":"70","author":"Dejas","year":"2023","journal-title":"Semin Immunol"},{"key":"B61","doi-asserted-by":"publisher","DOI":"10.1089\/wound.2012.0383","article-title":"Neutrophils and wound repair: Positive actions and negative reactions","volume":"2","author":"Wilgus","year":"2013","journal-title":"Adv Wound Care"},{"key":"B62","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1515\/hsz-2018-0224","article-title":"Physical plasma and leukocytes \u2013 immune or reactive","volume":"400","author":"Bekeschus","year":"2019","journal-title":"Biol Chem"},{"key":"B63","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.20852","article-title":"Functional and molecular characterization of NOS isoforms in rat neutrophil precursor cells","author":"Kumar","year":"2010","journal-title":"Cytometry Part A"},{"key":"B64","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2021.119018","article-title":"nNOS induction and NOSIP interaction impact granulopoiesis and neutrophil differentiation by modulating nitric oxide generation","volume":"1868","author":"Sadaf","year":"2021","journal-title":"Biochim Biophys Acta (BBA) - Mol Cell Res"},{"key":"B65","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2012.01.016","article-title":"Increased production of nitric oxide by neutrophils from patients with chronic granulomatous disease on interferon-gamma treatment","volume":"12","author":"Naderi beni","year":"2012","journal-title":"Int Immunopharmacol"},{"key":"B66","doi-asserted-by":"publisher","first-page":"593","DOI":"10.1164\/rccm.202305-0889OC","article-title":"Human neutrophils couple nitric oxide production and extracellular trap formation in allergic asthma","volume":"210","author":"Chac\u00f3n","year":"2024","journal-title":"Am J Respir Crit Care Med"},{"key":"B67","doi-asserted-by":"publisher","DOI":"10.3390\/ijms221810060","article-title":"Long-term interactions of circulating neutrophils with titanium implants, the role of platelets in regulation of leukocyte function","volume":"22","author":"Zdziennicka","year":"2021","journal-title":"Int J Mol Sci"},{"key":"B68","doi-asserted-by":"publisher","DOI":"10.3390\/ani16020193","article-title":"Mild ozone-induced oxidative stress modulates the activity and viability of porcine neutrophils and monocytes","volume":"16","author":"Nguyen Ngoc","year":"2026","journal-title":"Anim (Basel)"},{"key":"B69","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.00832","article-title":"Establishing porcine monocyte-derived macrophage and dendritic cell systems for studying the interaction with PRRSV-1","volume":"7","author":"Singleton","year":"2016","journal-title":"Front Microbiol"},{"key":"B70","doi-asserted-by":"publisher","DOI":"10.3390\/app16125802","article-title":"Antibacterial and antifungal activity of a cold atmospheric nitrogen plasma device in porcine burn and excisional wound models: A consolidated pilot evaluation","volume":"16","author":"Park","year":"2026","journal-title":"Appl Sci"},{"key":"B71","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2023.e22568","article-title":"Applications of cold atmospheric plasma in immune-mediated inflammatory diseases via redox homeostasis: Evidence and prospects","volume":"9","author":"Ma","year":"2023","journal-title":"Heliyon"},{"key":"B72","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-02-139634","article-title":"Neutrophil secretion products pave the way for inflammatory monocytes","volume":"112","author":"Soehnlein","year":"2008","journal-title":"Blood"},{"key":"B73","doi-asserted-by":"publisher","DOI":"10.1002\/adtp.202500097","article-title":"Cold plasma generates a localized inflammatory response and promotes muscle repair","volume":"8","author":"Smith","year":"2025","journal-title":"Adv Ther"},{"key":"B74","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2023.1240596","article-title":"Exploring the potential of cold plasma therapy in treating bacterial infections in veterinary medicine: Opportunities and challenges","volume":"10","author":"Mohseni","year":"2023","journal-title":"Front Vet Sci"},{"key":"B75","doi-asserted-by":"publisher","DOI":"10.3390\/jfb14060327","article-title":"The role of neutrophils in biomaterial-based tissue repair\u2014shifting paradigms","volume":"14","author":"Sousa","year":"2023","journal-title":"J Funct Biomaterials"},{"key":"B76","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2025.118001","article-title":"Biological solutions activated by cold plasma at atmospheric pressure: A new therapeutic approach for skin wound healing","volume":"186","author":"Roux","year":"2025","journal-title":"Biomedicine Pharmacotherapy"}],"container-title":["Frontiers in Immunology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fimmu.2026.1750122\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T05:28:09Z","timestamp":1786080489000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fimmu.2026.1750122\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,7]]},"references-count":76,"alternative-id":["10.3389\/fimmu.2026.1750122"],"URL":"https:\/\/doi.org\/10.3389\/fimmu.2026.1750122","relation":{},"ISSN":["1664-3224"],"issn-type":[{"value":"1664-3224","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,7]]},"article-number":"1750122"}}